| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-42761 | |||
| HRP | NBP1-42762 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB600-1131SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56508SS | |||
| Azide and BSA Free | NBP2-33213 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24702SS | |||
| Azide and BSA Free | NBP2-33256 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71027 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85951-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35089-20ul | |||
![Western Blot: IL-6 Antibody [NB600-1131] - Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NB600-1131-img0014.jpg "Western Blot: IL-6 Antibody [NB600-1131] - Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none. Image using the HRP form of this antibody.")
![Immunohistochemistry: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains and tumors were stained with anti-CD31, anti-IL-6, and anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal and tumor tissues. Bar represents 50?um. Zoom-in factor: 4 Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03050-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1131-img0015.jpg)
![Western Blot: IL-6 Antibody [NB600-1131] - Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band 21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NB600-1131-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. a Human brain ECs were treated with glioma-CM for 24 h, & cell lysates were subjected to multiplex cytokine array analysis. Left, a representative blot. Right, quantified dot intensity of most significantly changed cytokines. b Human microvascular brain ECs were treated with glioma-CM that were harvested from different human glioma cells. Cell lysates were immunoblotted. c Human microvascular brain ECs & tumor-associated ECs isolated from different GBM patients were subjected to immunoblot analysis. d Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains & tumors were stained with anti-CD31, anti-IL-6, & anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal & tumor tissues. Bar represents 50 μm. Zoom-in factor: 4 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29422647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1131_rabbit-polyclonal-il-6-antibody-310202416154648.jpg)
![Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-Western-Blot-NB100-56508-img0003.jpg "Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml."){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-BSA-Free-Flow-Cytometry-NB100-56508-img0012.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green represents isotype control; antibody red represents RANK antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-Flow-Cytometry-NB100-56508-img0005.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Using the PE direct conjugate An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-Flow-Cytometry-NB100-56508-img0009.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A [9A725] Antibody NB100-56508AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-Flow-Cytometry-NB100-56508-img0010.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on MG-63 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-9A725-BSA-Free-Flow-Cytometry-NB100-56508-img0011.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0009.jpg "Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence."){kind=icon}
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Formalin-fixed paraffin-embedded tissue sections of human liver were probed for RANK/TNFRSF11A mRNA (ACD RNAScope Probe, catalog # 412281; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NBP2-24702) at 5 ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to plasma membrane.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Dual-RNAscope-ISH-IHC-NBP2-24702-img0011.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from Raw 264.7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0010.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis of antibody on (a) untreated BALB/c mouse splenocytes and (b) BALB/c mouse splenocytes stimulated with 5 ug/ml ConA for 72 hours. Cells were stained with 2 ug of antibody per 1x10^6 cells. Green represents isotype control; red represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Flow-Cytometry-NBP2-24702-img0014.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Detection of RANK in RAW cells. Lane 1. detection of RANK in RAW, a mouse cell line. Lane 2 & 3. IP/analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or this antibody. RANK was detected by western blot using this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0001.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis on RAW cells using 5 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control; purple represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Flow-Cytometry-NBP2-24702-img0013.jpg){kind=icon}
![Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0010.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - IL-6 antibody detects IL-6 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.IL-6 stained by IL-6 antibody (NBP2-16957) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16957_rabbit-polyclonal-il-6-antibody-91020242011469.jpg)
![Western Blot: IL6 Antibody (3G9) [NBP1-47810] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 IL6 antibody (2G3) at 1:2000](http://images.novusbio.com/fullsize/IL6-Antibody-3G9-Western-Blot-NBP1-47810-img0002.jpg "Western Blot: IL6 Antibody (3G9) [NBP1-47810] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 IL6 antibody (2G3) at 1:2000")
![Immunocytochemistry/Immunofluorescence: IL-6 Antibody (3G9) [NBP1-47810] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL6.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunocytochemistry-Immunofluorescence-NBP1-47810-img0019.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human prostate tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0034.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0021.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0025.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human breast tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0027.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human colon tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0028.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0029.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human Kidney tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0030.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human breast tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720249362622.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human lung tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241003225.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human pancreas tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241003219.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human kidney tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241003236.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human lung tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241044651.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human ovary tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-1772024936263.jpg)
![Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241044633.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human Ovary tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-1772024959423.jpg)
![Immunocytochemistry/Immunofluorescence: CSK Antibody [NBP1-85951] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/CSK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85951-img0009.jpg "Immunocytochemistry/Immunofluorescence: CSK Antibody [NBP1-85951] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.")
![Western Blot: CSK Antibody [NBP1-85951] - Analysis in human cell line RT-4 and human cell line HeLa.](http://images.novusbio.com/fullsize/CSK-Antibody-Western-Blot-NBP1-85951-img0010.jpg)
![Immunohistochemistry-Paraffin: CSK Antibody [NBP1-85951] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CSK-Antibody-Immunohistochemistry-Paraffin-NBP1-85951-img0013.jpg)
![Immunohistochemistry-Paraffin: CSK Antibody [NBP1-85951] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in Purkinje cells and cells in granular layer.](http://images.novusbio.com/fullsize/CSK-Antibody-Immunohistochemistry-Paraffin-NBP1-85951-img0012.jpg)
![Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Western-Blot-NBP2-22527-img0006.jpg "Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunocytochemistry-Immunofluorescence-NBP2-22527-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0004.jpg)
![Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunoprecipitation-NBP2-22527-img0002.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0016.jpg "Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CSK Antibody (3A3) [H00001445-M01] - Analysis of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Immunohistochemistry-Paraffin-H00001445-M01-img0010.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi ( Cat # H00001445-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody (M01) clone 3A3 (Cat # H00001445-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0012.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - CSK monoclonal antibody (M01), clone 3A3 Analysis of CSK expression in HL-60.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0014.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - CSK monoclonal antibody (M01), clone 3A3. Analysis of CSK expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0015.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK transfected lysate using anti-CSK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CSK MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0017.jpg)
![ELISA: CSK Antibody (3A3) [H00001445-M01] - Detection limit for recombinant GST tagged CSK is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-ELISA-H00001445-M01-img0013.jpg)
![Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Western-Blot-NBP2-22526-img0001.jpg "Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0006.jpg)
![Immunohistochemistry: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 showing staining in the membrane of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunohistochemistry-NBP2-22526-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0003.jpg)
![Immunoprecipitation: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunoprecipitation-NBP2-22526-img0002.jpg)
![Western Blot: IL-27 Antibody [NB100-56389] - Analysis of IL-27 in A) Jurkat and B) NIH 3T3 cells using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NB100-56389-img0003.jpg "Western Blot: IL-27 Antibody [NB100-56389] - Analysis of IL-27 in A) Jurkat and B) NIH 3T3 cells using this antibody at 1 ug/ml.")
![Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-78132-img0002.jpg "Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-78132] - IHC analysis of a formalin fixed paraffin embedded tissue section of human appendix using 1:200 dilution of IL-6 antibody (NBP2-78132). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated membrane staining of glandular cells.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunohistochemistry-Paraffin-NBP2-78132-img0001.jpg)
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-10122024121014.jpg "Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-101220241224118.jpg)
![Western Blot: RAP1A Antibody [NBP3-35089] - Western blot analysis of lysates from wild type(WT) and RAP1A knockdown (KD) 293T cells, using [KO Validated] RAP1A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411552841.jpg)
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411592829.jpg)
![Western Blot: RAP1A Antibody [NBP3-35089] - Western blot analysis of various lysates using [KO Validated] RAP1A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411535893.jpg)