| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27148-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58360SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30424-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47783-0.1mg | |||
| IHC-Prediluted | NBP2-48371 | |||
![Western Blot: NPM1 Antibody (PD00-91) [NBP3-32669] - Western blot analysis of NPM1 on different lysates with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate Lane 4: C2C12 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32669) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-278202415242627.jpg "Western Blot: NPM1 Antibody (PD00-91) [NBP3-32669] - Western blot analysis of NPM1 on different lysates with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate Lane 4: C2C12 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32669) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32669) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-298202414161882.jpg)
![Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunocytochemistry analysis of LOVO cells labeling NPM1 with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-2920241114844.jpg)
![Flow Cytometry: NPM1 Antibody (PD00-91) [NBP3-32669] - Flow cytometric analysis of Daudi cells labeling NPM1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32669, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-2920241637216.jpg)
![Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-288202412442938.png "Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-PPP3CB antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29820241426262.png)
![Immunocytochemistry/ Immunofluorescence: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunocytochemistry analysis of MCF-7 cells labeling PPP3CB with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-2920241114810.png)
![Flow Cytometry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Flow cytometric analysis of MCF-7 cells labeling PPP3CB. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32848, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29202416372124.png)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202491704.jpg%3Fv=1666704274 "Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: HBO1 Antibody (1D9H9) [NBP3-27148] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024916212.jpg%3Fv=1666704274)
![Immunohistochemistry: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis of paraffin-embedded cervical cancer tissues using HBO1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202492021.jpg%3Fv=1666704274)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mouse mAb against MOLT4 (1), COS7 (2), F9 (3), HepG2 (4), PC-2 (5), U251 (6), MCF-7 (7), and NIH/3T3 (8) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024916234.jpg%3Fv=1666704274)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against human HBO1 (AA: 1-200) recombinant protein. (Expected MW is 48.1 kDa)](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024917033.jpg%3Fv=1666704274)
![Immunohistochemistry: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis of paraffin-embedded colon cancer tissues using HBO1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202491629.jpg%3Fv=1666704274)
![Western Blot: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Western-Blot-NBP2-54545-img0005.jpg "Western Blot: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).")
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54545-img0001.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54545-img0002.jpg)
![Protein Array: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TOP2A Antibody (TOP2A/1362). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Protein-Array-NBP2-54545-img0003.jpg)
![Western Blot: FE65 Antibody [NB110-58360] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY APBB1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APBB1.](http://images.novusbio.com/fullsize/FE65-Antibody-Western-Blot-NB110-58360-img0006.jpg "Western Blot: FE65 Antibody [NB110-58360] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY APBB1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APBB1.")
![Western Blot: FE65 Antibody [NB110-58360] - Protein on HEK 293 cell lysate.](http://images.novusbio.com/fullsize/FE65-Antibody-Western-Blot-NB110-58360-img0005.jpg)
![Western Blot: LEPRE1 Antibody [NBP2-30424] - Analysis in human cell lines U2OS and MCF-7 using anti-P3H1 antibody. Corresponding P3H1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-Western-Blot-NBP2-30424-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: LEPRE1 Antibody [NBP2-30424] - Analysis in human cell lines U2OS and MCF-7 using anti-P3H1 antibody. Corresponding P3H1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-Immunohistochemistry-Paraffin-NBP2-30424-img0011.jpg)
![Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424] - Staining of human cervix shows weak to moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-Immunohistochemistry-Paraffin-NBP2-30424-img0010.jpg)
![LEPRE1 Antibody [NBP2-30424] - staining of human placenta shows strong cytoplasmic positivity in decidual cells.](http://images.novusbio.com/fullsize/nbp2-30424_rabbit-polyclonal-lepre1-antibody-immunohistochemistry-paraffin-274202394413..jpg)
![LEPRE1 Antibody [NBP2-30424] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/nbp2-30424_rabbit-polyclonal-lepre1-antibody-immunohistochemistry-paraffin-274202394550..jpg)
![LEPRE1 Antibody [NBP2-30424] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/nbp2-30424_rabbit-polyclonal-lepre1-antibody-immunohistochemistry-paraffin-274202394737..jpg)
![Western Blot: COMMD7 Antibody [NBP1-31697] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/COMMD7-Antibody-Western-Blot-NBP1-31697-img0003.jpg "Western Blot: COMMD7 Antibody [NBP1-31697] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: COMMD7 Antibody [NBP1-31697] - Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COMMD7 protein stained by COMMD7 antibody diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/COMMD7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31697-img0004.jpg)
![Immunohistochemistry-Paraffin: COMMD7 Antibody [NBP1-31697] - Sample: Paraffin-embedded mouse lung. COMMD7 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/COMMD7-Antibody-Immunohistochemistry-Paraffin-NBP1-31697-img0005.jpg)
![Immunohistochemistry-Paraffin: COMMD7 Antibody [NBP1-31697] - Paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/COMMD7-Antibody-Immunohistochemistry-Paraffin-NBP1-31697-img0002.jpg)
![Immunohistochemistry: COMMD7 Antibody [NBP1-31697] - COMMD7 antibody detects Commd7 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. COMMD7 antibody (NBP1-31697) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-31697_rabbit-polyclonal-commd7-antibody-91020242075912.jpg)
![Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NBP2-16366-img0008.jpg "Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ERF Antibody [NBP2-16366] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NBP2-16366-img0006.jpg)
![Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366] - U373 xenograft, using ERF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ERF-Antibody-Immunohistochemistry-Paraffin-NBP2-16366-img0002.jpg)
![Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27197-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: CLASP2 Antibody [NBP1-21395] - Detection of Human CLASP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody NBP1-21395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CLASP2-Antibody-Western-Blot-NBP1-21395-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: CLASP2 Antibody [NBP1-21395] - Detection of Human CLASP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody NBP1-21395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: CLASP2 Antibody [NBP1-21395] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CLASP2-Antibody-Western-Blot-NBP1-21395-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CLASP2 Antibody [NBP1-21395] - Detection of human CLASP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody NBP1-21395 (lot NBP1-21395-2) used for IP at 6 ug per reaction. CLASP2 was also immunoprecipitated by a previous lot of this antibody (NBP1-21395-1) and rabbit anti-CLASP2 antibody NBP1-21394. For blotting immunoprecipitated CLASP2, NBP1-21395 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CLASP2-Antibody-Immunoprecipitation-NBP1-21395-img0008.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (CIP1/823) [NBP2-44527] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (CIP1/823).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-CIP1-823-Western-Blot-NBP2-44527-img0003.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (CIP1/823) [NBP2-44527] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (CIP1/823).")
![Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (CIP1/823) [NBP2-44527] - Analysis of HeLa cells labeling p21 with p21/CIP1/CDKN1A Antibody (CIP1/823) followed by Goat anti-Mouse IgG-CF555 (Red). Membrane stained with Phalloidin 488 (Green).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-CIP1-823-Immunocytochemistry-Immunofluorescence-NBP2-44527-img0004.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (CIP1/823) [NBP2-44527] - Human Lung Squamous Cell Carcinoma (SCC) stained with p21 Monoclonal Antibody (CIP1/823).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-CIP1-823-Immunohistochemistry-Paraffin-NBP2-44527-img0002.jpg)
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