| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85574-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25093-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72891 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56343SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56462SS | |||
![Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Western-Blot-NB200-335-img0006.jpg "Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunohistochemistry-Paraffin-NB200-335-img0008.jpg)
![Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-ASC2 antibody. For blotting immunoprecipitated ASC2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunoprecipitation-NB200-335-img0013.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-9102024209145.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420115062.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202419593519.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420131285.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242021927.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420134048.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242063358.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-33748] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-1610202419414590.jpg)
![BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (NBP1-33748) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-immunocytochemistry-immunofluorescence-261120251844101.jpg)
![Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in NIH/3T3.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Western-Blot-H00005245-M01-img0015.jpg "Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Immunocytochemistry-Immunofluorescence-H00005245-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Immunohistochemistry-Paraffin-H00005245-M01-img0011.jpg)
![Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2 Analysis of PHB expression in Hela.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Western-Blot-H00005245-M01-img0013.jpg)
![Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in PC-12.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Western-Blot-H00005245-M01-img0014.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Immunohistochemistry-Paraffin-H00005245-M01-img0009.jpg)
![Sandwich ELISA: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Detection limit for recombinant GST tagged PHB is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Sandwich-ELISA-H00005245-M01-img0012.jpg)
![Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - KO Validation in 293T Cells Loading: 10 ug of lysate Antibodies: beta Amyloid, 0.5 ug/mL and beta-actin, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-2711202419333416.gif "<b>Genetic Strategies Validation. </b>Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - KO Validation in 293T Cells Loading: 10 ug of lysate Antibodies: beta Amyloid, 0.5 ug/mL and beta-actin, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunohistochemistry of beta Amyloid in human brain tissue with beta Amyloid antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120241918631.gif)
![Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunohistochemistry of beta Amyloid in human brain (Alzheimer's disease) tissue with beta Amyloid antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120242016992.jpg)
![Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 ug/mL.Green: beta Amyloid Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120241939277.gif)
![Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - Western blot analysis of beta Amyloid in (A) human, (B) mouse, and (C) rat brain tissue lysates with beta Amyloid antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120242022942.jpg)
![Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] Analysis of extracts (35ug) from 9 different cell lines by using anti-N Cadherin monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Western-Blot-NBP2-01498-img0008.jpg "Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] Analysis of extracts (35ug) from 9 different cell lines by using anti-N Cadherin monoclonal antibody.")
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Human liver tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0007.jpg)
![Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-N Cadherin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Flow-Cytometry-NBP2-01498-img0002.jpg)
![Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY N Cadherin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-N Cadherin.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Western-Blot-NBP2-01498-img0003.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0004.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0005.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0006.jpg)
![Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - Analysis of Hela cells, using anti-N Cadherin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Flow-Cytometry-NBP2-01498-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183342.jpg "Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (1ug/ml) staining of HeLa (A) and (0.5ug/ml) MCF7 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-1652023918334.jpg)
![Flow Cytometry: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183312.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183347.jpg)
![Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183314.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (7ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183344.jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0008.jpg "Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Analysis using MAPK3 mAb against human ERK1 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0006.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis using ERK1 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0007.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg)
![ELISA: ERK1 Antibody (1E5) [NBP2-22203] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-ELISA-NBP2-22203-img0005.jpg)
![Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-56343-img0002.jpg "Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56343-img0004.jpg)
![Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Simple-Western-NB100-56343-img0003.jpg)
![Western Blot: GEF-H1 Antibody [NBP2-21577] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody diluted at 1:4000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-Western-Blot-NBP2-21577-img0028.jpg "Western Blot: GEF-H1 Antibody [NBP2-21577] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody diluted at 1:4000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody [NBP2-21577] - GEF-H1 antibody detects GEF-H1 protein at cytoskeleton and Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GEF-H1 stained by GEF-H1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21577-img0026.jpg)
![Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - GEF-H1 antibody detects GEF-H1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GEF-H1 stained by GEF-H1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-21577-img0027.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GEF-H1 Antibody [NBP2-21577] - Sample (10 ug of whole cell lysate) A: hMSC-3A6 B: siRNA of ARHGEF2/GEF-H1 8% SDS PAGE diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-Western-Blot-NBP2-21577-img0008.jpg)
![Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-21577-img0004.jpg)
![Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - Paraffin-embedded rat hind brain. GEF-H1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-21577-img0011.jpg)
![GEF-H1 antibody detects GEF-H1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse mouse cerebellum.Green: GEF-H1 stained by GEF-H1 antibody (NBP2-21577) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp2-21577_rabbit-polyclonal-gef-h1-antibody-immunohistochemistry-frozen-2611202518375023.jpg)
![Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Western-Blot-NB100-56462-img0004.jpg "Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: Smad2 Antibody [NB100-56462] - Mouse testis tissue probed with this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Immunohistochemistry-Paraffin-NB100-56462-img0003.jpg)