| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80971-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72891 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56462SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70454 | |||
![Western Blot: SPARC Antibody [NBP1-80971] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80971-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SPARC Antibody [NBP1-80971] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: SPARC Antibody [NBP1-80971] - Analysis using Anti-SPARC antibody NBP1-80971 (A) shows similar pattern to independent antibody NBP1-80972 (B).](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80971-img0010.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human placenta shows moderate cytoplasmic positivity in fibroblasts.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0013.jpg)
![Western Blot: SPARC Antibody [NBP1-80971] - Analysis in control (vector only transfected HEK293T lysate) and SPARC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80971-img0009.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0011.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0012.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human skin shows moderate cytoplasmic positivity in fibroblasts.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0014.jpg)
![Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] Analysis of extracts (35ug) from 9 different cell lines by using anti-N Cadherin monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Western-Blot-NBP2-01498-img0008.jpg "Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] Analysis of extracts (35ug) from 9 different cell lines by using anti-N Cadherin monoclonal antibody.")
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Human liver tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0007.jpg)
![Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-N Cadherin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Flow-Cytometry-NBP2-01498-img0002.jpg)
![Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY N Cadherin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-N Cadherin.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Western-Blot-NBP2-01498-img0003.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0004.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0005.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0006.jpg)
![Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - Analysis of Hela cells, using anti-N Cadherin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Flow-Cytometry-NBP2-01498-img0001.jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0008.jpg "Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Analysis using MAPK3 mAb against human ERK1 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0006.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis using ERK1 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0007.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg)
![ELISA: ERK1 Antibody (1E5) [NBP2-22203] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-ELISA-NBP2-22203-img0005.jpg)
![Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Western-Blot-NBP2-67587-img0008.jpg "Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human testis tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0010.jpg)
![Flow Cytometry: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Flow-Cytometry-NBP2-67587-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of paraffin-embedded mouse kidney tissue using anti-Wilms Tumor Protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0009.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Western-Blot-NB100-56462-img0004.jpg "Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: Smad2 Antibody [NB100-56462] - Mouse testis tissue probed with this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Immunohistochemistry-Paraffin-NB100-56462-img0003.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19459] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19459-img0021.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody [NBP2-19459] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19459-img0019.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19459] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19459-img0014.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19459] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19459-img0023.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Human hepatoma. N-Cadherin antibody [N1N3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19459-img0008.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Rat liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19459-img0018.jpg)
![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (NBP2-19459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19459_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251842291.jpg)
![N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: N-Cadherin stained by N-Cadherin antibody [N1N3] (NBP2-19459) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19459_rabbit-polyclonal-n-cadherin-antibody-immunocytochemistry-immunofluorescence-261120251825260.jpg)
![N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis.A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract5% SDS-PAGEN-Cadherin antibody [N1N3] (NBP2-19459) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19459_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251829017.jpg)
![Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01] - LEPRE1 monoclonal antibody (M01), clone 3C7 Analysis of LEPRE1 expression in HeLa.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-3C7-Western-Blot-H00064175-M01-img0011.jpg "Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01] - LEPRE1 monoclonal antibody (M01), clone 3C7 Analysis of LEPRE1 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of monoclonal antibody to LEPRE1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-3C7-Immunocytochemistry-Immunofluorescence-H00064175-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 6 ug/ml.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-3C7-Immunohistochemistry-Paraffin-H00064175-M01-img0009.jpg)
![Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody (M01), clone 3C7.Lane 1: LEPRE1 transfected lysate(46 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-3C7-Western-Blot-H00064175-M01-img0012.jpg)
![Sandwich ELISA: LEPRE1 Antibody (3C7) [H00064175-M01] - Detection limit for recombinant GST tagged LEPRE1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-3C7-Sandwich-ELISA-H00064175-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Analysis in human testis and liver tissues using NBP1-84416 antibody. Corresponding RBM14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Analysis in human testis and liver tissues using NBP1-84416 antibody. Corresponding RBM14 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RBM14 Antibody [NBP1-84416] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84416-img0009.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0013.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0011.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0012.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0014.jpg)
![Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Western-Blot-NBP2-76420-img0001.jpg "Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF by Simple Western™. Samples: Whole cell lysate (2.0 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311310.jpg)
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311923.jpg)
![Immunocytochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE MCF-7 cells by ICC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunocytochemistry-NBP2-76420-img0003.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0004.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) and rabbit anti-CTCF (NB500-177).](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunoprecipitation-NBP2-76420-img0005.jpg)
![Flow Cytometry-CTCF Antibody (BLR041F) [NBP2-76420] -Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] or isotype control (unshaded). Secondary: DyLight® 650-conjugatedgoat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-flow-cytometry-287202312246..jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Western-Blot-NBP1-47708-img0008.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Crystallin AB.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunocytochemistry-Immunofluorescence-NBP1-47708-img0011.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human pancreas tissue using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunohistochemistry-Paraffin-NBP1-47708-img0013.jpg)
![Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Crystallin AB antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Flow-Cytometry-NBP1-47708-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of HT29 cells using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunocytochemistry-Immunofluorescence-NBP1-47708-img0010.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human endometrium tissue using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunohistochemistry-Paraffin-NBP1-47708-img0012.jpg)
![Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunoprecipitation-NBP1-47708-img0014.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).](http://images.novusbio.com/fullsize/nbp1-47708_mouse-alphab-crystallin-cryab-mab-oti6a9-17720241044629.jpg)
