| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78133SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89095-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44199 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38851-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-181SS | |||
![Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Western-Blot-NBP2-78133-img0002.jpg "Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NBP2-78133] - IHC analysis of a FFPE tissue section of the mouse testis using 1:200 dilution of SCP3 antibody (NBP2-78133). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated strong staining associated with chromatin in meiotic cells.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-Paraffin-NBP2-78133-img0001.jpg)
![Western Blot: TPX2 Antibody (18D5-1) [NB100-74556] - Analysis of TPX2.](http://images.novusbio.com/fullsize/TPX2-Antibody-18D5-1-Western-Blot-NB100-74556-img0002.jpg "Western Blot: TPX2 Antibody (18D5-1) [NB100-74556] - Analysis of TPX2.")
![Immunohistochemistry-Paraffin: TPX2 Antibody (18D5-1) [NB100-74556] - Biopsies of deparaffinized Human testis tissue.](http://images.novusbio.com/fullsize/TPX2-Antibody-18D5-1-Immunohistochemistry-Paraffin-NB100-74556-img0003.jpg)
![Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-288202412442960.jpg "Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32753) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-298202414161863.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1 with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202410334043.jpg)
![Flow Cytometry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Flow cytometric analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32753, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202416372140.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0005.jpg "Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.")
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0006.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0002.jpg)
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0007.jpg)
![Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0021.jpg "Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PLK1 (NB100-547). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-547-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 on HeLa whole cell lysate using NB100-547. B. Affinity purified rabbit anti-PLK1 antibodies NB100-546, NB100-547, and NB100-548. IPed PLK1 was detected using NB100-548.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0020.jpg)
![Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Western-Blot-H00000983-M04-img0011.jpg "Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in NIH/3T3 (Cat # L018V1).")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Immunohistochemistry-Paraffin-H00000983-M04-img0007.jpg)
![Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Western-Blot-H00000983-M04-img0008.jpg)
![Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Western-Blot-H00000983-M04-img0009.jpg)
![Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Western-Blot-H00000983-M04-img0010.jpg)
![ELISA: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Detection limit for recombinant GST tagged CDC2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-ELISA-H00000983-M04-img0006.jpg)
![Western Blot: Cdc27 Antibody [NBP1-89095] - Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cdc27-Antibody-Western-Blot-NBP1-89095-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cdc27 Antibody [NBP1-89095] - Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cdc27 Antibody [NBP1-89095] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cdc27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89095-img0006.jpg)
![Immunohistochemistry-Paraffin: Cdc27 Antibody [NBP1-89095] - Staining of human bronchus shows strong nuclear positivity in respiratory epithelial cells.](http://images.novusbio.com/fullsize/Cdc27-Antibody-Immunohistochemistry-Paraffin-NBP1-89095-img0002.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human testis and endometrium tissues using NBP2-88942 antibody. Corresponding SPAG5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human testis and endometrium tissues using NBP2-88942 antibody. Corresponding SPAG5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Western-Blot-NBP2-88942-img0006.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human endometrium shows very weak cytoplasmic positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0001.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human prostate shows no cytoplasmic positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0002.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0003.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CCNB3 Antibody [NBP1-89475] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CCNB3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89475-img0009.jpg "Immunocytochemistry/Immunofluorescence: CCNB3 Antibody [NBP1-89475] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CCNB3 Antibody [NBP1-89475] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CCNB3-Antibody-Immunohistochemistry-Paraffin-NBP1-89475-img0014.jpg)
![Immunohistochemistry-Paraffin: CCNB3 Antibody [NBP1-89475] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CCNB3-Antibody-Immunohistochemistry-Paraffin-NBP1-89475-img0013.jpg)
![Immunohistochemistry-Paraffin: CCNB3 Antibody [NBP1-89475] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CCNB3-Antibody-Immunohistochemistry-Paraffin-NBP1-89475-img0015.jpg)
![Western Blot: SMC3 Antibody [NBP1-88715] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP1-88715-img0004.jpg "Western Blot: SMC3 Antibody [NBP1-88715] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG")
![Immunohistochemistry: SMC3 Antibody [NBP1-88715] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunohistochemistry-NBP1-88715-img0003.jpg)
![Simple Western: SMC3 Antibody [NBP1-88715] - Simple Western lane view shows a specific band for SMC3 in 0.2 mg/ml of U-251 MG lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.](http://images.novusbio.com/fullsize/SMC3-Antibody-Simple-Western-NBP1-88715-img0005.jpg)
![Simple Western: SMC3 Antibody [NBP1-88715] - Electropherogram image of the corresponding Simple Western lane view. SMC3 antibody was used at 1:25 dilution on U-251 MG lysate(s) respectively.](http://images.novusbio.com/fullsize/SMC3-Antibody-Simple-Western-NBP1-88715-img0006.jpg)
![Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/STAG3-Antibody-Immunohistochemistry-Paraffin-NBP2-38851-img0003.jpg "Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining of human testis shows high expression.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining in human testis and stomach tissues using anti-STAG3 antibody. Corresponding STAG3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/STAG3-Antibody-Immunohistochemistry-Paraffin-NBP2-38851-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining of human cerebral cortex, kidney, stomach and testis using Anti-STAG3 antibody NBP2-38851 (A) shows similar protein distribution across tissues to independent antibody NBP2-47258 (B).](http://images.novusbio.com/fullsize/STAG3-Antibody-Immunohistochemistry-Paraffin-NBP2-38851-img0005.jpg)
![Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/STAG3-Antibody-Immunohistochemistry-Paraffin-NBP2-38851-img0006.jpg)
![Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining of human kidney.](http://images.novusbio.com/fullsize/STAG3-Antibody-Immunohistochemistry-Paraffin-NBP2-38851-img0007.jpg)
![Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining of human stomach using Anti-STAG3 antibody NBP2-38851.](http://images.novusbio.com/fullsize/STAG3-Antibody-Immunohistochemistry-Paraffin-NBP2-38851-img0008.jpg)
![Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining of human testis using Anti-STAG3 antibody NBP2-38851.](http://images.novusbio.com/fullsize/STAG3-Antibody-Immunohistochemistry-Paraffin-NBP2-38851-img0009.jpg)
![Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.](http://images.novusbio.com/fullsize/DMC1-Antibody-1D12-4-Western-Blot-NB100-181-img0003.jpg "Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.")