| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61656-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90058-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33953-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33954-25ul | |||
![Western Blot: CDK4 Antibody (3G7Q7) [NBP3-15344] - Western blot analysis of extracts of MCF7 cells, using CDK4 Rabbit mAb (NBP3-15344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Western-Blot-NBP3-15344-img0008.jpg "Western Blot: CDK4 Antibody (3G7Q7) [NBP3-15344] - Western blot analysis of extracts of MCF7 cells, using CDK4 Rabbit mAb (NBP3-15344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunofluorescence analysis of HeLa cells using CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunocytochemistry-Immunofluorescence-NBP3-15344-img0007.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0005.jpg)
![Western Blot: CDK4 Antibody (6N3M6) [CDK4] - Western blot analysis of various lysates, using CDK4 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-52202517162385.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [CDK4] - Immunohistochemistry analysis of paraffin-embedded Human prostate tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-52202517284930.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-72202512435398.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245317.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245373.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245387.jpg)
![Western Blot: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Analysis of extracts of various cell lines, using p27/Kip1 antibody (NBP3-15662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-9R5B4-Western-Blot-NBP3-15662-img0003.jpg "Western Blot: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Analysis of extracts of various cell lines, using p27/Kip1 antibody (NBP3-15662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Rat ovary using p27/Kip1 Rabbit mAb (NBP3-15662) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-9R5B4-Immunohistochemistry-Paraffin-NBP3-15662-img0001.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517315585.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-5220251734546.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-5220251734555.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517333867.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517304988.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-5220251731107.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517363713.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517311074.jpg)
![Western Blot: Apc2 Antibody (3A2G2) [NBP2-61656] - Analysis using APC2 mAb against human APC2 (AA: 2041-2181) recombinant protein. (Expected MW is 40.9 kDa)](http://images.novusbio.com/fullsize/Apc2-Antibody-3A2G2-Western-Blot-NBP2-61656-img0006.jpg "Western Blot: Apc2 Antibody (3A2G2) [NBP2-61656] - Analysis using APC2 mAb against human APC2 (AA: 2041-2181) recombinant protein. (Expected MW is 40.9 kDa)")
![Immunocytochemistry/Immunofluorescence: Apc2 Antibody (3A2G2) [NBP2-61656] - Analysis of Hela cells using APC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Apc2-Antibody-3A2G2-Immunocytochemistry-Immunofluorescence-NBP2-61656-img0005.jpg)
![Immunohistochemistry: Apc2 Antibody (3A2G2) [NBP2-61656] - Analysis of paraffin-embedded bladder cancer tissues using APC2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Apc2-Antibody-3A2G2-Immunohistochemistry-NBP2-61656-img0004.jpg)
![Flow Cytometry: Apc2 Antibody (3A2G2) [NBP2-61656] - Analysis of Hela cells using APC2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Apc2-Antibody-3A2G2-Flow-Cytometry-NBP2-61656-img0002.jpg)
![Western Blot: Apc2 Antibody (3A2G2) [NBP2-61656] - Analysis using APC2 mAb against HEK293 (1) and APC2 (AA: 2041-2181)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Apc2-Antibody-3A2G2-Western-Blot-NBP2-61656-img0007.jpg)
![Immunohistochemistry: Apc2 Antibody (3A2G2) [NBP2-61656] - Analysis of paraffin-embedded ovarian cancer tissues using APC2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Apc2-Antibody-3A2G2-Immunohistochemistry-NBP2-61656-img0003.jpg)
![ELISA: Apc2 Antibody (3A2G2) [NBP2-61656] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Apc2-Antibody-3A2G2-ELISA-NBP2-61656-img0001.jpg)
![Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate.](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Western-Blot-NBP2-37566-img0003.jpg "Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate.")
![Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MCM2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Immunohistochemistry-NBP2-37566-img0006.jpg)
![Flow Cytometry: MCM2 Antibody (2B3) [NBP2-37566] - Flow cytometric analysis of Hela cells using MCM2 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Flow-Cytometry-NBP2-37566-img0004.jpg)
![Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mAb against human MCM2 (AA: 16-232) recombinant protein.(Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Western-Blot-NBP2-37566-img0002.jpg)
![Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Immunohistochemistry-NBP2-37566-img0005.jpg)
![ELISA: MCM2 Antibody (2B3) [NBP2-37566] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-ELISA-NBP2-37566-img0001.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0004.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human breast carcinoma with rabbit monoclonal antibody at 1:200).](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0001.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human liver with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0003.jpg)
![Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Wee1-Antibody-Immunohistochemistry-Paraffin-NBP1-33506-img0003.jpg "Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Wee1 Antibody [NBP1-33506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Wee1-Antibody-Western-Blot-NBP1-33506-img0007.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-2611202518191318.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251830124.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000 and competitor's antibody (CST#13084) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251829022.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-26112025196217.jpg)
![Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-NBP1-21374-img0039.jpg "<b>Biological Strategies Validation. </b>Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: E2F-4 Antibody [NBP1-21374] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunohistochemistry-NBP1-21374-img0037.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: E2F-4 Antibody [NBP1-21374] - Detection of human E2F4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for IP at 6 ug per reaction. E2F4 was also immunoprecipitated by another rabbit anti-E2F4 antibody. For blotting immunoprecipitated E2F4, NBP1-21374 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunoprecipitation-NBP1-21374-img0040.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: E2F-4 Antibody [NBP1-21374] - Immunoblots of indicated proteins in OVCAR-8 and PEA1 cells transfected with control luciferase (Luc) and two independent E2F-4 siRNAs. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6789141/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Knockdown-Validated-NBP1-21374-img0041.jpg)
![Western Blot: Geminin Antibody (1A8) [H00051053-M01] - Analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody (M01), clone 1A8.Lane 1: GMNN transfected lysate(23.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-Western-Blot-H00051053-M01-img0012.jpg "Western Blot: Geminin Antibody (1A8) [H00051053-M01] - Analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody (M01), clone 1A8.Lane 1: GMNN transfected lysate(23.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-Immunocytochemistry-Immunofluorescence-H00051053-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-Immunohistochemistry-Paraffin-H00051053-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-Immunohistochemistry-Paraffin-H00051053-M01-img0008.jpg)
![ELISA: Geminin Antibody (1A8) [H00051053-M01] - Detection limit for recombinant GST tagged GMNN is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Geminin-Antibody-1A8-ELISA-H00051053-M01-img0013.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MCM2 and Phospho-MCM2. Lower Panel: Total MCM2.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0003.jpg "Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MCM2 and Phospho-MCM2. Lower Panel: Total MCM2.")
![Immunocytochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE OVCAR-4 cells by ICC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunocytochemistry-NBP3-07021-img0006.jpg)
![Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) in FFPE mouse renal cell carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunohistochemistry-NBP3-07021-img0005.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of HDLM-2, HeLa, Jurkat, MCF-7, MOLT-4, Malme-3M, HEK293T, and Raji lysate. Antibody: Rabbit anti-Phospho-MCM2 recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0001.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) by WB of BW5147.3, TCMK-1, mIMCD-3, A20, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0002.jpg)
![Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunohistochemistry-NBP3-07021-img0004.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Analysis in human lymph node and liver tissues. Corresponding MCM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Analysis in human lymph node and liver tissues. Corresponding MCM2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MCM2 Antibody [NBP2-33953] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NBP2-33953-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody [NBP2-33953] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33953-img0005.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human pancreas shows low positivity in exocrine glanular cells as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0011.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0012.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0013.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining in human lymph node and kidney tissues using anti-MCM2 antibody. Corresponding MCM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining in human lymph node and kidney tissues using anti-MCM2 antibody. Corresponding MCM2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MCM2 Antibody [NBP2-33954] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NBP2-33954-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody [NBP2-33954] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33954-img0011.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0016.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0009.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0017.jpg)