| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15566-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47933-0.1mg | |||
| IHC-Prediluted | NBP2-48381 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26515-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47934-0.1mg | |||
| IHC-Prediluted | NBP2-48369 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59625 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47765-0.1mg | |||
| IHC-Prediluted | NBP2-48424 | |||
![Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Western-Blot-NBP3-15566-img0002.jpg "Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Chromatin Immunoprecipitation: HDAC1 Antibody (2W4I5) [NBP3-15566] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody (NBP3-15566) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Chromatin-Immunoprecipitation-NBP3-15566-img0003.jpg)
![Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.](http://images.novusbio.com/fullsize/PLK1-[p-Ser482--p-Ser486--p-Ser490]-Antibody-Western-Blot-NB300-278-img0001.jpg "Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.")
![Western Blot: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-5G6-Western-Blot-NBP1-51669-img0006.jpg "Western Blot: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-5G6-Immunocytochemistry-NBP1-51669-img0009.jpg)
![Flow Cytometry: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-5G6-Flow-Cytometry-NBP1-51669-img0007.jpg)
![Immunohistochemistry-Paraffin: G-CSF Antibody (SPM468) [NBP2-44797] - Human Tonsil stained with G-CSF Monoclonal Antibody (SPM468).](http://images.novusbio.com/fullsize/G-CSF-Antibody-SPM468-Immunohistochemistry-Paraffin-NBP2-44797-img0003.jpg "Immunohistochemistry-Paraffin: G-CSF Antibody (SPM468) [NBP2-44797] - Human Tonsil stained with G-CSF Monoclonal Antibody (SPM468).")
![Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60kDa for Cyclin A.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NB600-495-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60kDa for Cyclin A.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NB600-495] - Anti-Cyclin A antibody was diluted 1:500 to detect Cyclin A in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunohistochemistry-Paraffin-NB600-495-img0002.jpg)
![Western Blot: Cdc23 Antibody [NB100-59829] - Detection of human and mouse CDC23/APC8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Cdc23-Antibody-Western-Blot-NB100-59829-img0003.jpg "Western Blot: Cdc23 Antibody [NB100-59829] - Detection of human and mouse CDC23/APC8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cdc23 Antibody [NB100-59829] - Detection of Human and Mouse CDC23/APC8 on HeLa whole cell lysate using NB100-59829. CDC23/APC8 was also immunoprecipitated by rabbit anti-CDC23/APC8 antibody NB100-59830.](http://images.novusbio.com/fullsize/Cdc23-Antibody-Western-Blot-NB100-59829-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Cdc23 Antibody [NB100-59829] - Detection of human CDC23/APC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for IP at 3 ug per reaction. CDC23/APC8 was also immunoprecipitated by another rabbit anti-CDC23/APC8 antibody. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Cdc23-Antibody-NB100-59829-img0004.jpg)
![Western Blot: CDC45L Antibody [NBP1-32772] - Sample (30 ug of whole cell lysate)A: H12997. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Western-Blot-NBP1-32772-img0004.jpg "Western Blot: CDC45L Antibody [NBP1-32772] - Sample (30 ug of whole cell lysate)A: H12997. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CDC45L Antibody [NBP1-32772] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC45L protein stained by CDC45L antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32772-img0003.jpg)
![Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] - Image of AIF Antibody (4B2) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26515_rabbit-aif-mab-4b2-262202415253322.jpg "Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] - Image of AIF Antibody (4B2) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] - Image of AIF Antibody (4B2) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26515_rabbit-aif-mab-4b2-26220241522419.jpg)
![Western Blot: AIF Antibody (4B2) [NBP3-26515] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Rat liver tissue, Rat kidney tissue. All lanes: AIF Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 67, 36, 29, 27 kDa Observed band size: 67 kDa](http://images.novusbio.com/fullsize/nbp3-26515_rabbit-aif-mab-4b2-282202411363023.jpg)
![Immunohistochemistry-Paraffin: G-CSF Antibody (CSF3/900) [NBP2-44798] - Human Tonsil stained with G-CSF Monoclonal Antibody (CSF3/900). Note specific cytoplasmic staining of granulocytes.](http://images.novusbio.com/fullsize/G-CSF-Antibody-CSF3-900-Immunohistochemistry-Paraffin-NBP2-44798-img0001.jpg "Immunohistochemistry-Paraffin: G-CSF Antibody (CSF3/900) [NBP2-44798] - Human Tonsil stained with G-CSF Monoclonal Antibody (CSF3/900). Note specific cytoplasmic staining of granulocytes.")
![Protein Array: p53 Antibody (PAb122) [NBP2-59624] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-PAb122-Protein-Array-NBP2-59624-img0001.jpg "Protein Array: p53 Antibody (PAb122) [NBP2-59624] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29")
![Immunohistochemistry-Paraffin: p57 Kip2 Antibody (SPM308) [NBP2-44489] - Human Prostate Carcinoma stained with p57 Monoclonal Antibody (SPM308).](http://images.novusbio.com/fullsize/p57-Kip2-Antibody-SPM308-Immunohistochemistry-Paraffin-NBP2-44489-img0001.jpg "Immunohistochemistry-Paraffin: p57 Kip2 Antibody (SPM308) [NBP2-44489] - Human Prostate Carcinoma stained with p57 Monoclonal Antibody (SPM308).")
![Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-61065-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: HDAC6 Antibody [NB100-61065] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-61065-img0004.jpg)
![Immunoprecipitation: HDAC6 Antibody [NB100-61065] - Detection of human HDAC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 (lot NB100-61065-2) used for IP at 6 ug per reaction. HDAC6 was also immunoprecipitated by a previous lot of this antibody NB100-61065-1. For blotting immunoprecipitated HDAC6,NB100-61065 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunoprecipitation-NB100-61065-img0009.jpg)