| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89193-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76498-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52480-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52481-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37351-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04967-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: NCOA6 Antibody [NBP1-89193] - Staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89193-img0006.jpg "Immunocytochemistry/Immunofluorescence: NCOA6 Antibody [NBP1-89193] - Staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: NCOA6 Antibody [NBP1-89193] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunohistochemistry-Paraffin-NBP1-89193-img0010.jpg)
![Immunohistochemistry-Paraffin: NCOA6 Antibody [NBP1-89193] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunohistochemistry-Paraffin-NBP1-89193-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCOA6 Antibody [NBP1-89193] - Staining in human testis and skeletal muscle tissues.. Corresponding NCOA6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunohistochemistry-Paraffin-NBP1-89193-img0008.jpg)
![Immunohistochemistry-Paraffin: NCOA6 Antibody [NBP1-89193] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunohistochemistry-Paraffin-NBP1-89193-img0009.jpg)
![Immunohistochemistry-Paraffin: NCOA6 Antibody [NBP1-89193] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunohistochemistry-Paraffin-NBP1-89193-img0011.jpg)
![Western Blot: SLC39A1 Antibody [NBP1-76498] - Mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SLC39A1-Antibody-Western-Blot-NBP1-76498-img0001.jpg "Western Blot: SLC39A1 Antibody [NBP1-76498] - Mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: SLC39A1 Antibody [NBP1-76498] - Human kidney tissue at 2.5 ug/mL. dilution](http://images.novusbio.com/fullsize/SLC39A1-Antibody-Immunohistochemistry-NBP1-76498-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: SLC39A1 Antibody - BSA Free [NBP1-76498] - Immunofluorescence of SLC39A1 in human kidney tissue with SLC39A1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76498_rabbit-polyclonal-slc39a1-antibody-2711202420272442.jpg)
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2451-img0002.jpg "Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NB100-2451-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2451-img0001.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451] - Staining of paraffin embedded Human Cortex. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NB100-2451-img0003.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP2-16380-img0002.jpg "Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP2-16380] - Human intestine. ERR alpha stained by ERR alpha antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP2-16380-img0006.jpg)
![Chromatin Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Chromatin-Immunoprecipitation-NBP2-16380-img0003.jpg)
![Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 ug of ERR alpha antibody 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary eagent.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunoprecipitation-NBP2-16380-img0005.jpg)
![Western Blot: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, HeLa, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD7 antibody NBP1-28727 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP1-28727-img0008.jpg "Western Blot: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, HeLa, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD7 antibody NBP1-28727 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRD7 (NBP1-28727) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BRD7-Antibody-Immunohistochemistry-Paraffin-NBP1-28727-img0007.jpg)
![Immunoprecipitation: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD7 antibody NBP1-28727 (lot NBP1-28727-2) used for IP at 6 ug per reaction. BRD7 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-28727-1). For blotting immunoprecipitated BRD7, NBP1-28727 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/BRD7-Antibody-Immunoprecipitation-NBP1-28727-img0009.jpg)
![Western Blot: M-CSF Antibody [NBP1-59425] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/M-CSF-Antibody-Western-Blot-NBP1-59425-img0008.jpg "Western Blot: M-CSF Antibody [NBP1-59425] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry-Paraffin: M-CSF Antibody [NBP1-59425] - Human Placenta Tissue, 10 ug/ml](http://images.novusbio.com/fullsize/M-CSF-Antibody-Immunohistochemistry-Paraffin-NBP1-59425-img0004.jpg)
![Western Blot: M-CSF Antibody [NBP1-59425] - WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: COLO205 cell lysate](http://images.novusbio.com/fullsize/M-CSF-Antibody-Western-Blot-NBP1-59425-img0005.jpg)
![Western Blot: M-CSF Antibody [NBP1-59425] - Expressed in Human 721_B cells.](http://images.novusbio.com/fullsize/M-CSF-Antibody-Western-Blot-NBP1-59425-img0006.jpg)
![Western Blot: M-CSF Antibody [NBP1-59425] - Antibody Titration: 1 ug/ml Human heart.](http://images.novusbio.com/fullsize/M-CSF-Antibody-Western-Blot-NBP1-59425-img0007.jpg)
![Western Blot: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Western blot analysis of extracts of various cell lines, using Oncostatin M/OSM Rabbit mAb (NBP3-16686) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Oncostatin-M-OSM-Antibody-8O8K9-Western-Blot-NBP3-16686-img0004.jpg "Western Blot: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Western blot analysis of extracts of various cell lines, using Oncostatin M/OSM Rabbit mAb (NBP3-16686) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Immunofluorescence analysis of Jurkat cells using Oncostatin M/OSM Rabbit mAb (NBP3-16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Oncostatin-M-OSM-Antibody-8O8K9-Immunocytochemistry-Immunofluorescence-NBP3-16686-img0001.jpg)
![Immunohistochemistry: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Immunofluorescence analysis of mouse spleen using Oncostatin M/OSM Rabbit mAb (NBP3-16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Oncostatin-M-OSM-Antibody-8O8K9-Immunohistochemistry-NBP3-16686-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Oncostatin M/OSM Antibody (8O8K9) [NBP3-16686] - Immunofluorescence analysis of paraffin-embedded rat spleen using Oncostatin M/OSM Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16686_rabbit-oncostatin-m-osm-mab-8o8k9-52202511383752.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-Western-Blot-NBP2-52480-img0003.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)")
![Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Analysis of paraffin-embedded endometrial cancer tissues using HDAC4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-Immunohistochemistry-NBP2-52480-img0002.jpg)
![Flow Cytometry: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Analysis of HeLa cells using HDAC4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-Flow-Cytometry-NBP2-52480-img0001.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Analysis using HDAC4 mAb against HEK293 (1) and HDAC4 (AA: 456-592)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-Western-Blot-NBP2-52480-img0004.jpg)
![ELISA: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-ELISA-NBP2-52480-img0005.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-Western-Blot-NBP2-52481-img0003.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Analysis of HeLa cells using HDAC4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-Immunofluorescence-NBP2-52481-img0002.jpg)
![Flow Cytometry: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Analysis of HeLa cells using HDAC4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-Flow-Cytometry-NBP2-52481-img0001.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Analysis using HDAC4 mAb against HEK293 (1) and HDAC4 (AA: 456-592)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-Western-Blot-NBP2-52481-img0004.jpg)
![ELISA: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-ELISA-NBP2-52481-img0005.jpg)
![Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mouse mAb against PANC1 (1) and PC-3 (2) cell lysate.](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Western-Blot-NBP2-37351-img0004.jpg "Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mouse mAb against PANC1 (1) and PC-3 (2) cell lysate.")
![Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using GPNMB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Immunohistochemistry-NBP2-37351-img0006.jpg)
![Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mAb against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Western-Blot-NBP2-37351-img0002.jpg)
![Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mAb against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Western-Blot-NBP2-37351-img0003.jpg)
![Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPNMB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-Immunohistochemistry-NBP2-37351-img0005.jpg)
![ELISA: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Osteoactivin-GPNMB-Antibody-7C10E5-ELISA-NBP2-37351-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967] - Staining of methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/HDAC4+5+9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-04967-img0001.jpg "Immunocytochemistry/Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967] - Staining of methanol-fixed Hela cells.")
![Western Blot: HDAC4+5+9 Antibody [NBP1-04967] - Western blot analysis of extracts from 293 and Hela cells using NBP1-04967](http://images.novusbio.com/fullsize/nbp1-04967_rabbit-polyclonal-hdac4-5-9-antibody-23420241523251.jpg)
![Immunohistochemistry-Paraffin: HDAC4+5+9 Antibody [NBP1-04967] - Paraffin-embedded human lung carcinoma tissue using NBP1-04967(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nbp1-04967_rabbit-polyclonal-hdac4-5-9-antibody-103202523464514.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-278202416331227.jpg "Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32350) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29820241435263.jpg)
![Immunocytochemistry/ Immunofluorescence: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunocytochemistry analysis of U-87 MG cells labeling FRA-1/FOSL1 with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202410484854.jpg)
![Flow Cytometry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Flow cytometric analysis of U-87 MG cells labeling FRA-1/FOSL1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32350, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202416453260.jpg)