| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52636-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87321-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90047-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90013-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40823SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0010.jpg "Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0006.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human endometrial tissue with Rabbit anti-c-jun [p Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0012.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0005.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Western blot analysis of c-jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0007.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submited by a verified customer review.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0008.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun (p Ser63) on different lysates with Rabbit anti-c-Jun (p Ser63) antibody at 1/500 dilution. Lane 1: U87MG cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-c-jun [Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0011.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-418_rabbit-polyclonal-neurofibromin-1-antibody-western-blot-132202618215117.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92100_rabbit-polyclonal-mat2a-antibody-western-blot-13220261739820.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0016.jpg "Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Immunocytochemistry-Immunofluorescence-H00002965-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Immunohistochemistry-Paraffin-H00002965-M01-img0011.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 over-expressed 293 cell line, cotransfected with GTF2H1 Validated Chimera RNAi ( Cat # H00002965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (Cat # H00002965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0012.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Analysis of GTF2H1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0014.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Analysis of GTF2H1 expression in Jurkat.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0015.jpg)
![Sandwich ELISA: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Detection limit for recombinant GST tagged GTF2H1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Sandwich-ELISA-H00002965-M01-img0013.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0012.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0007.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0008.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0010.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human prostate shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0011.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NPM1 Antibody [NBP1-90047] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90047-img0007.jpg "Immunocytochemistry/Immunofluorescence: NPM1 Antibody [NBP1-90047] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunohistochemistry-Paraffin-NBP1-90047-img0010.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunohistochemistry-Paraffin-NBP1-90047-img0008.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunohistochemistry-Paraffin-NBP1-90047-img0009.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NPM1-Antibody-Immunohistochemistry-Paraffin-NBP1-90047-img0011.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human liver shows very weak positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0013.jpg "Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human liver shows very weak positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells and in cells in granular and molecular layer.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0011.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SNF5 Antibody [NBP1-90013] - Analysis using Anti-SMARCB1 antibody NBP1-90013 (A) shows similar pattern to independent antibody NBP1-90014 (B).](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NBP1-90013-img0009.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Western-Blot-NBP2-37576-img0003.jpg "Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Flow Cytometry: c-jun Antibody (5B1) [NBP2-37576] - Analysis of HepG2 cells using c-Jun mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Flow-Cytometry-NBP2-37576-img0005.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mAb against human c-Jun (AA: 199-331) recombinant protein.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Western-Blot-NBP2-37576-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human intima cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunohistochemistry-NBP2-37576-img0006.jpg)
![ELISA: c-jun Antibody (5B1) [NBP2-37576] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-ELISA-NBP2-37576-img0001.jpg)
![Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP2-24693-img0006.jpg "Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NBP2-24693] - Immunofluorescence of BMI1 in human oral squamous cell carcinoma (UPCI:SCC029B) cells. These cells are part of a large collection of HNSCC (head and neck SCC) lines established by the Gollin lab (White et al., 2007). The BMI1 antibody was used at 5 ug/ml. Staining is primarily nuclear, some cytoplasmic staining is also observed. Data courtesy of Hatem Kaseb, MD and Susanne M. Gollin, PhD.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24693-img0004.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24693-img0005.jpg)
![Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle](http://images.novusbio.com/fullsize/Bmi1-Antibody-Western-Blot-NB110-40823-img0002.jpg "Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle")
![Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Bmi1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40823-img0004.jpg)
![Western Blot: CtIP Antibody [NB100-79810] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-CtIP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.](http://images.novusbio.com/fullsize/CtIP-Antibody-Western-Blot-NB100-79810-img0006.jpg "Western Blot: CtIP Antibody [NB100-79810] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-CtIP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CtIP Antibody [NB100-79810] - Detection of human CtIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CtIP antibody NB100-79810 used for IP at 3 ug per reaction. CtIP was also immunoprecipitated by rabbit anti-CtIP antibody NB100-79809. For blotting immunoprecipitated CtIP, NB100-79810 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CtIP-Antibody-Immunoprecipitation-NB100-79810-img0007.jpg)
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)