| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43889 | |||
| BSA Free | NBP2-34477-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43887 | |||
| BSA Free | NBP2-34475-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88312-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB500-182SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54587-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50039SS | |||
![Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0012.jpg "Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Immunohistochemistry-Paraffin-H00000701-M02-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi ( Cat # H00000701-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody (M02) clone 2G5 (Cat # H00000701-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0009.jpg)
![Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - BUB1B monoclonal antibody (M02), clone 2G5 Analysis of BUB1B expression in Hela S3 NE.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0011.jpg)
![Proximity Ligation Assay: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Proximity-Ligation-Assay-H00000701-M02-img0006.jpg)
![Sandwich ELISA: BubR1 Antibody (2G5) [H00000701-M02] - Detection limit for recombinant GST tagged BUB1B is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Sandwich-ELISA-H00000701-M02-img0010.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: E-Cadherin Antibody (CL1180) [NBP2-34477] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Western-Blot-NBP2-34477-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of BEWO cells using the Anti-CDH1 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunocytochemistry-Immunofluorescence-NBP2-34477-img0015.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of human duodenum shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0010.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0011.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of human colorectal cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0012.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0013.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Western-Blot-NBP2-34475-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of BEWO cells using the Anti-CDH1 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunocytochemistry-Immunofluorescence-NBP2-34475-img0015.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human duodenum shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0010.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0011.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human colorectal cancer shows moderate to strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0012.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NBP1-88312] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88312-img0014.jpg "Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NBP1-88312] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunohistochemistry-Paraffin-NBP1-88312-img0017.jpg)
![Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunohistochemistry-Paraffin-NBP1-88312-img0020.jpg)
![Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human duodenum shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunohistochemistry-Paraffin-NBP1-88312-img0018.jpg)
![Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human skin shows strong nuclear positivity in basal cells.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunohistochemistry-Paraffin-NBP1-88312-img0019.jpg)
![Western Blot: NuMA Antibody [NBP2-54672] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/NuMA-Antibody-Western-Blot-NBP2-54672-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NuMA Antibody [NBP2-54672] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human fallopian tube, gastrointestinal, kidney and lymphoid tissues using Anti-NUMA1 antibody NBP2-54672 (A) shows similar protein distribution across tissues to independent antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-108202395858..jpg)
![Immunocytochemistry/Immunofluorescence: NuMA Antibody [NBP2-54672] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54672-img0003.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-108202310024..jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-108202310540..jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human tonsil shows strong nuclear positivity in non-germinal center cells and germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-10820231078..jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-108202310820..jpg)
![Western Blot: ch-TOG Antibody [NB500-182] - Specificity of polyclonal antibodies against ch-TOGp. Total cell protein (50 mg) from human HeLa cells was immunoblotted with a rabbit polyclonal serum generated against the C-terminal 301 amino acids of the ch-TOGp.](http://images.novusbio.com/fullsize/ch-TOG-Antibody-Western-Blot-NB500-182-img0002.jpg "Western Blot: ch-TOG Antibody [NB500-182] - Specificity of polyclonal antibodies against ch-TOGp. Total cell protein (50 mg) from human HeLa cells was immunoblotted with a rabbit polyclonal serum generated against the C-terminal 301 amino acids of the ch-TOGp.")
![Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229] - Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Antibody (4A2).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Western-Blot-NBP2-53229-img0005.jpg "Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229] - Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Antibody (4A2).")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (4A2) [NBP2-53229] - Immunofluorescence analysis of MeOH-fixed MCF-7 cells. E-Cadherin Mouse Monoclonal Antibody (4A2) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Immunocytochemistry-Immunofluorescence-NBP2-53229-img0007.jpg)
![Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229] - Staining of human pancreatic tumor. Image from a verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Immunohistochemistry-NBP2-53229-img0003.jpg)
![Flow Cytometry: E-Cadherin Antibody (4A2) [NBP2-53229] - Flow Cytometric Analysis of human trypsinized MCF-7 cells using E-Cadherin Antibody (4A2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Flow-Cytometry-NBP2-53229-img0006.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) [NBP2-53229] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (4A2).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Immunohistochemistry-Paraffin-NBP2-53229-img0001.jpg)
![Western Blot: MCRS1 Antibody [H00010445-B01P] - Western blot analysis of MCRS1 expression in whole testis extracts from 4.5, 6.5, 9.5, 12.5, and 15.5 dpp and adult mice. Each lane was loaded with 50 ug of testis protein extracts. Alpha tubulin was used as internal control. Image collected and cropped by CiteAb from the following publication (//link.springer.com/article/10.1007/s00412-017-0631-z) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MCRS1-Antibody-Western-Blot-H00010445-B01P-img0007.jpg "Western Blot: MCRS1 Antibody [H00010445-B01P] - Western blot analysis of MCRS1 expression in whole testis extracts from 4.5, 6.5, 9.5, 12.5, and 15.5 dpp and adult mice. Each lane was loaded with 50 ug of testis protein extracts. Alpha tubulin was used as internal control. Image collected and cropped by CiteAb from the following publication (//link.springer.com/article/10.1007/s00412-017-0631-z) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: MCRS1 Antibody [H00010445-B01P] - Analysis of purified antibody to MCRS1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/MCRS1-Antibody-Immunocytochemistry-Immunofluorescence-H00010445-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: MCRS1 Antibody [H00010445-B01P] - Analysis of purified antibody to MCRS1 on formalin-fixed paraffin-embedded human spleen. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/MCRS1-Antibody-Immunohistochemistry-Paraffin-H00010445-B01P-img0005.jpg)
![Western Blot: MCRS1 Antibody [H00010445-B01P] - Analysis of MCRS1 expression in transfected 293T cell line by MCRS1 polyclonal antibody. Lane 1: MCRS1 transfected lysate(50.82 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCRS1-Antibody-Western-Blot-H00010445-B01P-img0006.jpg)
![Western Blot: MCRS1 Antibody [H00010445-B01P] - Identification of interacting partners of PRDM9 by Y2H screening. a Schematic representation of the domain structure of seven PRDM9-interacting proteins identified by Y2H screening. The prey fragments identified in the screens are shown as blue bars underneath each protein. The length (number of amino acid residues) of each full-length protein is indicated on the right. b RNA expression of the PRDM9-interacting proteins in mouse testes (left) & ovaries (right) during meiosis. Data were extracted from RNA-seq data in Margolin et al. (2014) & Soh et al. (2015)). The average gene expression is shown in reads per kilobase per million of reads (RPKM) & fragments per kilobase of transcript per million mapped read (FKPM) for testes & ovaries, respectively. Zoomed-in views of the dashed line boxes are shown below. Spo11 & Spata19 expression were used as positive & negative controls, respectively. c Western blot analysis of PRDM9, CXXC1, PIH1D1, CEP70, & MCRS1 expression in whole testis extracts from 4.5, 6.5, 9.5, 12.5, & 15.5 dpp & adult mice. Each lane was loaded with 50 μg of testis protein extracts. Tubulin alpha was used as internal control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28527011), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010445-b01p_mouse-polyclonal-mcrs1-antibody-310202415483646.jpg)
![Western Blot: NuMA Antibody (6D4I4) [NBP3-16411] - Western blot analysis of extracts of various cell lines, using NuMA1 Rabbit mAb (NBP3-16411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/NuMA-Antibody-6D4I4-Western-Blot-NBP3-16411-img0004.jpg "Western Blot: NuMA Antibody (6D4I4) [NBP3-16411] - Western blot analysis of extracts of various cell lines, using NuMA1 Rabbit mAb (NBP3-16411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded mouse testis tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-72202512432040.jpg)
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded rat lung tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-72202512432086.jpg)
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded mouse intestin tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-7220251245350.jpg)
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded human thyroid cancer tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-72202512432093.jpg)
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded mouse liver tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-7220251245341.jpg)
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded rat brain tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-72202512432097.jpg)
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded human spleen tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-7220251243539.jpg)
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded mouse lung tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-7220251243530.jpg)
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded rat colon tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-72202512432047.jpg)
![Immunohistochemistry: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry analysis of NuMA in paraffin-embedded mouse spleen tissue using NuMA Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16411_rabbit-numa-mab-6d4i4-72202512435334.jpg)
![Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Western-Blot-NBP2-50039-img0006.jpg "Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - High magnification confocal immunofluorescence of HeLa cells stained with NBP2-50039, diluted 1:1,000 in green and counterstained with antibody to vimentin at 1:1,000 dilution in red. Blue is the DNA stain DAPI revealing the nuclei. The MCA-3F11 reveals aurora B localized in midbodies, midzones of dividing cells and also in the nuclei or some cells.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Immunocytochemistry-Immunofluorescence-NBP2-50039-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - HeLa cell cultures were stained with NBP2-50039 (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Immunocytochemistry-Immunofluorescence-NBP2-50039-img0003.jpg)
![Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP2-36737-img0001.jpg "Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.")
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP2-36737] - Analysis of FFPE section of human testis using Aurora A antibody at 10 ug/ml concentration. Heat-induced antigen retrieval was employed in this IHC-P validation.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP2-36737-img0002.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Human BubR1 on HeLa whole cell lysate using NB100-579. BubR1 was also immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0002.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0001.jpg)