| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72106 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73481 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37736-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44069 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56554SS | |||
| Azide and BSA Free | NBP2-80914 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06705SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-154SS | |||
| Azide and BSA Free | NBP2-80871 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
![Western Blot: CCNB1IP1 Antibody (4H3) [NBP2-00600] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB1IP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB1IP1.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Western-Blot-NBP2-00600-img0003.jpg "Western Blot: CCNB1IP1 Antibody (4H3) [NBP2-00600] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB1IP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB1IP1.")
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] Staining of paraffin-embedded Human prostate tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0008.jpg)
![Flow Cytometry: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Analysis of Jurkat cells, using anti-CCNB1IP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Flow-Cytometry-NBP2-00600-img0002.jpg)
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0004.jpg)
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0005.jpg)
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Staining of paraffin-embedded Human lymph node tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0006.jpg)
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Staining of paraffin-embedded Human pancreas tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0007.jpg)
![Flow Cytometry: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Analysis of Hela cells, using anti-CCNB1IP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Flow-Cytometry-NBP2-00600-img0001.jpg)
![Western Blot: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMS2.](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Western-Blot-NBP2-46459-img0008.jpg "Western Blot: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMS2.")
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0007.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0002.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0003.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0004.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0005.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0006.jpg)
![Immunofluorescence: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunofluorescence-NBP2-46459-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0008.jpg "Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.")
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HepG2 cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0003.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0007.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: Human; HeLa: Human; HT29: Human; A549: Human; COS7: monkey; Jurkat: human; MDCK: Canine; PC12: Rat; MCF7: human)](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0004.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0005.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0006.jpg)
![Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7. Analysis of CHEK2 expression in HeLa.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Western-Blot-H00011200-M01-img0011.jpg "Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7. Analysis of CHEK2 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Immunocytochemistry-Immunofluorescence-H00011200-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Immunohistochemistry-Paraffin-H00011200-M01-img0009.jpg)
![Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7 Analysis of CHEK2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Western-Blot-H00011200-M01-img0012.jpg)
![Sandwich ELISA: Chk2 Antibody (4B7) [H00011200-M01] - Detection limit for recombinant GST tagged CHEK2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Sandwich-ELISA-H00011200-M01-img0010.jpg)
![Western Blot: MKKS Antibody [NBP1-92123] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/MKKS-Antibody-Western-Blot-NBP1-92123-img0006.jpg "Western Blot: MKKS Antibody [NBP1-92123] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: MKKS Antibody [NBP1-92123] - Immunofluorescent staining of human cell line U-2 OS shows localization to centrosome.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92123-img0008.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0012.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human colon, kidney, testis and urinary bladder using Anti-MKKS antibody NBP1-92123 (A) shows similar protein distribution across tissues to independent antibody NBP1-92122 (B).](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0009.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human kidney.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0010.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human testis.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0011.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human colon.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0013.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-92123_rabbit-polyclonal-mkks-antibody-295202516101016.jpg)
![Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Western-Blot-NBP2-88933-img0008.jpg "Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of MCF7 cells using the Anti-CHEK2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunocytochemistry-Immunofluorescence-NBP2-88933-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human testis and cerebral cortex tissues using NBP2-88933 antibody. Corresponding CHEK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0007.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0002.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0003.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human human embryonal carcinoma of testis shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis cancer, seminoma shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0006.jpg)
![Western Blot: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of PMS2 using this antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate.](http://images.novusbio.com/fullsize/PMS2-Antibody-163C1251-Western-Blot-NB100-56554-img0003.jpg "Western Blot: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of PMS2 using this antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate.")
![Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using PMS2 antibody at 5 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PMS2 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/PMS2-Antibody-163C1251-Immunohistochemistry-Paraffin-NB100-56554-img0005.jpg)
![Western Blot: Nbs1 Antibody [NBP1-06705] - Western Blot analysis of Nbs1 on NIH/3T3 (Lane 6) and HeLa whole cell extract (Lane 7) with Nbs1 Antibody [NBP1-06705]. Observed molecular weight at ~99 kDa.](http://images.novusbio.com/fullsize/NBS1-Antibody-Western-Blot-NBP1-06705-img0004.jpg "Western Blot: Nbs1 Antibody [NBP1-06705] - Western Blot analysis of Nbs1 on NIH/3T3 (Lane 6) and HeLa whole cell extract (Lane 7) with Nbs1 Antibody [NBP1-06705]. Observed molecular weight at ~99 kDa.")
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06705] - Staining of paraffin-embedded mouse skin with Nbs1 Antibody [NBP1-06705].](http://images.novusbio.com/fullsize/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06705-img0003.jpg)
![Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27b) [NB300-154] - Staining of adult human dorsal root ganglia.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27b-Immunohistochemistry-Paraffin-NB300-154-img0002.jpg "Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27b) [NB300-154] - Staining of adult human dorsal root ganglia.")
![Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Human colon carcinoma. Rb antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/RB1-Antibody-Immunohistochemistry-Paraffin-NBP2-20127-img0005.jpg "Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Human colon carcinoma. Rb antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min")
![Immunoprecipitation: RB1 Antibody [NBP2-20127] - HeLa whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of retinoblastoma 1 protein by 4 ug Rb1 antibody 7.5 % SDS-PAGE The immunoprecipitated retinoblastoma 1 protein was detected by Rb1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/RB1-Antibody-Immunoprecipitation-NBP2-20127-img0004.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)