| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85723-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08805 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89657-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56867-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22196-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47893-0.1mg | |||
| IHC-Prediluted | NBP2-48117 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70383 | |||
![Immunocytochemistry/Immunofluorescence: CDC45L Antibody [NBP1-85723] - Staining of human cell line A-431 shows localization to nucleoplasm & centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85723-img0005.jpg "Immunocytochemistry/Immunofluorescence: CDC45L Antibody [NBP1-85723] - Staining of human cell line A-431 shows localization to nucleoplasm & centrosome. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723] - Staining in human testis and endometrium tissues using anti-CDC45 antibody. Corresponding CDC45 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Immunohistochemistry-Paraffin-NBP1-85723-img0012.jpg)
![Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Immunohistochemistry-Paraffin-NBP1-85723-img0010.jpg)
![Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Immunohistochemistry-Paraffin-NBP1-85723-img0011.jpg)
![Western Blot: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 by WB of HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and HCT 116 lysate. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Western-Blot-NBP2-76409-img0001.jpg "Western Blot: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 by WB of HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and HCT 116 lysate. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 in FFPE breast carcinoma cell line MCF-7 by ICC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Immunocytochemistry-NBP2-76409-img0004.jpg)
![Immunohistochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 in FFPE human ovarian carcinoma by IHC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Immunohistochemistry-NBP2-76409-img0003.jpg)
![Flow Cytometry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-3D4] NBP2-76409 (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Flow-Cytometry-NBP2-76409-img0005.jpg)
![Immunoprecipitation: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409).](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Immunoprecipitation-NBP2-76409-img0002.jpg)
![Western Blot: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Western Blot Analysis of human Jurkat cell lysate using Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-2460-Western-Blot-NBP3-07484-img0004.jpg "Western Blot: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Western Blot Analysis of human Jurkat cell lysate using Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).")
![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-2460-Immunohistochemistry-Paraffin-NBP3-07484-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-2460-Immunohistochemistry-Paraffin-NBP3-07484-img0002.jpg)
![Protein Array: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-2460-Protein-Array-NBP3-07484-img0001.jpg)
![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-07484] - Cyclin E1 stained with CODEX in tonsil tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp3-07484_mouse-monoclonal-cyclin-e1-antibody-ccne1-2460-1772024944231.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody [NBP1-89657] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89657-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody [NBP1-89657] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-89657] - Staining in human bone marrow and cerebral cortex tissues using anti-CCNB2 antibody. Corresponding CCNB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-89657-img0011.jpg)
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-89657] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-89657-img0009.jpg)
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-89657] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-89657-img0010.jpg)
![Western Blot: Cyclin B1 Antibody [NBP2-56867] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Western-Blot-NBP2-56867-img0003.jpg "Western Blot: Cyclin B1 Antibody [NBP2-56867] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody [NBP2-56867] - Staining of human cell line A549 shows localization to cytosol.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56867-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867] - Staining in human testis and pancreas tissues using anti-CCNB1 antibody. Corresponding CCNB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56867-img0007.jpg)
![Immunohistochemistry: Cyclin B1 Antibody [NBP2-56867] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in subset of glandular cells.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunohistochemistry-NBP2-56867-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56867-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56867-img0006.jpg)
![Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Western-Blot-NBP2-22196-img0006.jpg "Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: PLK1 Antibody (3C11) [NBP2-22196] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PLK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Immunohistochemistry-Paraffin-NBP2-22196-img0003.jpg)
![Flow Cytometry: PLK1 Antibody (3C11) [NBP2-22196] - Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Flow-Cytometry-NBP2-22196-img0001.jpg)
![Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mAb against human PLK1 recombinant protein. (Expected MW is 45.7 kDa)](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Western-Blot-NBP2-22196-img0005.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (3C11) [NBP2-22196] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PLK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Immunohistochemistry-Paraffin-NBP2-22196-img0002.jpg)
![ELISA: PLK1 Antibody (3C11) [NBP2-22196] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-ELISA-NBP2-22196-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunocytochemistry-Immunofluorescence-NBP2-44687-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Endometrial Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0001.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Ovarian Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Prostate Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Testicular Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0004.jpg)
![Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM7-Antibody-Western-Blot-NB100-252-img0082.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: MCM7 Antibody [NB100-252] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-Western-Blot-NB100-252-img0002.jpg)
![Western Blot: Cdc6 Antibody (1A4) [NBP2-46192] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.](http://images.novusbio.com/fullsize/Cdc6-Antibody-1A4-Western-Blot-NBP2-46192-img0007.jpg "Western Blot: Cdc6 Antibody (1A4) [NBP2-46192] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.")
![Immunohistochemistry: Cdc6 Antibody (1A4) [NBP2-46192] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-1A4-Immunohistochemistry-NBP2-46192-img0006.jpg)
![Immunohistochemistry: Cdc6 Antibody (1A4) [NBP2-46192] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-1A4-Immunohistochemistry-NBP2-46192-img0001.jpg)
![Immunohistochemistry: Cdc6 Antibody (1A4) [NBP2-46192] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-1A4-Immunohistochemistry-NBP2-46192-img0002.jpg)
![Immunohistochemistry: Cdc6 Antibody (1A4) [NBP2-46192] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-1A4-Immunohistochemistry-NBP2-46192-img0003.jpg)
![Immunohistochemistry: Cdc6 Antibody (1A4) [NBP2-46192] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-1A4-Immunohistochemistry-NBP2-46192-img0004.jpg)
![Immunohistochemistry: Cdc6 Antibody (1A4) [NBP2-46192] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-1A4-Immunohistochemistry-NBP2-46192-img0005.jpg)
![Western Blot: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Western-Blot-NBP2-70383-img0001.jpg "Western Blot: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.")
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0006.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0002.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0003.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0004.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0005.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0007.jpg)
![Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-288202412442960.jpg "Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32753) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-298202414161863.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1 with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202410334043.jpg)
![Flow Cytometry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Flow cytometric analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32753, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202416372140.jpg)
![Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0021.jpg "Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PLK1 (NB100-547). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-547-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 on HeLa whole cell lysate using NB100-547. B. Affinity purified rabbit anti-PLK1 antibodies NB100-546, NB100-547, and NB100-548. IPed PLK1 was detected using NB100-548.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0020.jpg)