| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12892-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99081-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92118-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55474-25ul | |||
![Western Blot: CENPC Antibody (5E3K5) [NBP3-16305] - Western blot analysis of extracts of various cell lines, using CENPC Rabbit mAb (NBP3-16305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CENPC-Antibody-5E3K5-Western-Blot-NBP3-16305-img0002.jpg "Western Blot: CENPC Antibody (5E3K5) [NBP3-16305] - Western blot analysis of extracts of various cell lines, using CENPC Rabbit mAb (NBP3-16305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: CENPC Antibody (5E3K5) [NBP3-16305] - Immunofluorescence analysis of C6 cells using CENPC Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16305_rabbit-cenpc-mab-5e3k5-822025209870.jpg)
![Western Blot: Nuf2 Antibody [NBP2-32211] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32211 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Western-Blot-NBP2-32211-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Nuf2 Antibody [NBP2-32211] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32211 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Nuf2 Antibody [NBP2-32211] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32211 used for IP at 6 ug per reaction. For blotting immunoprecipitated NUF2, NBP2-32211 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunoprecipitation-NBP2-32211-img0002.jpg)
![Western Blot: CENPA Antibody [NBP1-52937] - Titration: 1.25ug/ml Positive Control: Jurkat cell lysate.](http://images.novusbio.com/fullsize/CENPA-Antibody-Western-Blot-NBP1-52937-img0003.jpg "Western Blot: CENPA Antibody [NBP1-52937] - Titration: 1.25ug/ml Positive Control: Jurkat cell lysate.")
![Immunohistochemistry-Paraffin: CENPA Antibody [NBP1-52937] - Human Skin Tissue Squamous epithelial cells (Indicated with Arrows), 4-8ug/ml.](http://images.novusbio.com/fullsize/CENPA-Antibody-Immunohistochemistry-Paraffin-NBP1-52937-img0002.jpg)
![Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 . Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/CENPA-Antibody-5A7-2E11-Western-Blot-NBP2-12892-img0002.jpg "Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 . Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892] - Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/CENPA-Antibody-5A7-2E11-Immunocytochemistry-Immunofluorescence-NBP2-12892-img0003.jpg)
![Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Nuf2-Antibody-Western-Blot-NBP2-99081-img0002.jpg "Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Nuf2 Antibody [NBP2-99081] - Nuf2 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-Nuf2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Nuf2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunoprecipitation-NBP2-99081-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CENPB Antibody [NB100-2369] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-CENP-B used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/CENPB-Antibody-Immunohistochemistry-NB100-2369-img0004.jpg "Immunocytochemistry/Immunofluorescence: CENPB Antibody [NB100-2369] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-CENP-B used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.")
![Immunocytochemistry/Immunofluorescence: CENPB Antibody [NB100-2369] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-CENP-B used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/CENPB-Antibody-Immunohistochemistry-NB100-2369-img0003.jpg)
![Western Blot: CENPH Antibody [H00064946-B01P] - Analysis of CENPH expression in transfected 293T cell line by CENPH polyclonal antibody. Lane 1: CENPH transfected lysate(27.28 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CENPH-Antibody-Western-Blot-H00064946-B01P-img0003.jpg "Western Blot: CENPH Antibody [H00064946-B01P] - Analysis of CENPH expression in transfected 293T cell line by CENPH polyclonal antibody. Lane 1: CENPH transfected lysate(27.28 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CENPH Antibody [H00064946-B01P] - Analysis of purified antibody to CENPH on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/CENPH-Antibody-Immunocytochemistry-Immunofluorescence-H00064946-B01P-img0002.jpg)
![Western Blot: MIS12 Antibody [H00079003-B02P] - Analysis of MIS12 expression in transfected 293T cell line by MIS12 polyclonal antibody. Lane 1: MIS12 transfected lysate(22.55 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MIS12-Antibody-Western-Blot-H00079003-B02P-img0002.jpg "Western Blot: MIS12 Antibody [H00079003-B02P] - Analysis of MIS12 expression in transfected 293T cell line by MIS12 polyclonal antibody. Lane 1: MIS12 transfected lysate(22.55 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MIS12 Antibody [H00079003-B02P] - Analysis of purified antibody to MIS12 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/MIS12-Antibody-Immunocytochemistry-Immunofluorescence-H00079003-B02P-img0001.jpg)
![ELISA: Rat CENPE ELISA Kit (Colorimetric) [NBP2-75230] - Samples were spiked with high concentrations of Rat CENPE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75230_rat-cenpe-elisa-kit-colorimetric-132202416184845.jpg)
![ELISA: Rat CENPE ELISA Kit (Colorimetric) [NBP2-75230] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75230_rat-cenpe-elisa-kit-colorimetric-43202495017.jpg)
![Immunohistochemistry-Paraffin: MIS12 Antibody [NBP1-92118] - Staining of human spleen shows nuclear positivity in cells in red and white pulp.](http://images.novusbio.com/fullsize/MIS12-Antibody-Immunohistochemistry-Paraffin-NBP1-92118-img0004.jpg "Immunohistochemistry-Paraffin: MIS12 Antibody [NBP1-92118] - Staining of human spleen shows nuclear positivity in cells in red and white pulp.")
![Immunocytochemistry/Immunofluorescence: CENPC Antibody [NBP2-55474] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies, cytokinetic bridge, midbody, midbody ring & cleavage furrow.](http://images.novusbio.com/fullsize/CENPC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55474-img0002.jpg "Immunocytochemistry/Immunofluorescence: CENPC Antibody [NBP2-55474] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies, cytokinetic bridge, midbody, midbody ring & cleavage furrow.")
![Western Blot: Nuf2 Antibody (644) [NBP2-43779] - Analysis of whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDCA1 antibody [644] at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Nuf2-Antibody-644-Western-Blot-NBP2-43779-img0001.jpg "Western Blot: Nuf2 Antibody (644) [NBP2-43779] - Analysis of whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDCA1 antibody [644] at a dilution of 1:1000.")
![Immunoprecipitation: Nuf2 Antibody (644) [NBP2-43779] - Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 ug of NUF2 antibody [644]. Western blot analysis was performed using NUF2 antibody [644]. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Nuf2-Antibody-644-Immunoprecipitation-NBP2-43779-img0003.jpg)