| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21579-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80964 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27709-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82523-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90172-25ul | |||
![Immunohistochemistry: SASH1 Antibody [NBP1-26651] - Sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and lightchain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/SASH1-Antibody-Immunohistochemistry-NBP1-26651-img0004.jpg "Immunohistochemistry: SASH1 Antibody [NBP1-26651] - Sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and lightchain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.")
![Immunoprecipitation: SASH1 Antibody [NBP1-26651] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 3 mcg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti- SASH1 antibody NBP1-26650, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/SASH1-Antibody-Immunoprecipitation-NBP1-26651-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Immunofluorescent analysis of Jurkat cells treated with Pervanadate, using NBP3-21579.](http://images.novusbio.com/fullsize/nbp3-21579_rabbit-jak2-p-tyr-1007-mab-sr1129-5720231526114.jpg "Immunocytochemistry/Immunofluorescence: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Immunofluorescent analysis of Jurkat cells treated with Pervanadate, using NBP3-21579.")
![Immunohistochemistry: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Immunohistochemical analysis of paraffin-embedded human cervix cancer, using NBP3-21579.](http://images.novusbio.com/fullsize/nbp3-21579_rabbit-jak2-p-tyr-1007-mab-sr1129-57202315545129.jpg)
![Western Blot: Jak2 [p Tyr 1007] Antibody (SR1129) [NBP3-21579] - Western blot analysis using NBP3-21579 in Jurkat cell lysates treated with Pervanadate.](http://images.novusbio.com/fullsize/nbp3-21579_rabbit-jak2-p-tyr-1007-mab-sr1129-5720231649421.jpg)
![Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182) antibody.](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells, treated with TNFa or calf intestinal phosphatase (CIP).](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0005.jpg)
![Immunohistochemistry-Paraffin: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82097(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82097_rabbit-polyclonal-p38-alpha-p-tyr182-antibody-10320252332107.jpg)
![Immunohistochemistry-Paraffin: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - STAM antibody [HL1635] detects STAM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. STAM stained by STAM antibody [HL1635] (NBP3-25518) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25518_rabbit-stam-1-mab-hl1635-azide-and-bsa-free-2120249342764.jpg "Immunohistochemistry-Paraffin: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - STAM antibody [HL1635] detects STAM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. STAM stained by STAM antibody [HL1635] (NBP3-25518) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - STAM antibody [HL1635] detects STAM protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: STAM stained by STAM antibody [HL1635] (NBP3-25518) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25518_rabbit-stam-1-mab-hl1635-azide-and-bsa-free-2120249432856.jpg)
![Western Blot: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody [HL1635] (NBP3-25518) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25518_rabbit-stam-1-mab-hl1635-azide-and-bsa-free-2120249371538.jpg)
![Western Blot: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody [HL1635] (NBP3-25518) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25518_rabbit-stam-1-mab-hl1635-azide-and-bsa-free-212024940870.jpg)
![Western Blot: Calmodulin Antibody (10W3Y7) [NBP3-16500] - Western blot analysis of extracts of various cell lines, using Calmodulin Rabbit mAb (NBP3-16500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-10W3Y7-Western-Blot-NBP3-16500-img0002.jpg "Western Blot: Calmodulin Antibody (10W3Y7) [NBP3-16500] - Western blot analysis of extracts of various cell lines, using Calmodulin Rabbit mAb (NBP3-16500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (10W3Y7) [NBP3-16500] - Confocal imaging of paraffin-embedded Rat brain using Calmodulin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16500_rabbit-calmodulin-mab-10w3y7-5220251138924.jpg)
![Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (10W3Y7) [Calmodulin] - Confocal imaging of paraffin-embedded Mouse brain using Calmodulin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16500_rabbit-calmodulin-mab-10w3y7-52202517162361.jpg)
![Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-288202412442950.jpg "Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue untreated / treated with lambda pp / phospho-peptide / non-phospho-peptide with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-298202414333624.jpg)
![Immunocytochemistry/ Immunofluorescence: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunocytochemistry analysis of Jurkat cells treated with or without UV for 1 hour labeling p38 alpha with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-2920241114829.jpg)
![Western Blot: STAM-1 Antibody (29C678) [NB100-56533] - Western blot analysis of HeLa lysate (35ug per lane, RIPA buffer) using STAM antibody (NB100-56533) at 1ug/ml. Band observed at ~65kDA. (Expected MW of 59.2kDa according to NP_003464.1).](http://images.novusbio.com/fullsize/STAM-1-Antibody-29C678-Western-Blot-NB100-56533-img0002.jpg "Western Blot: STAM-1 Antibody (29C678) [NB100-56533] - Western blot analysis of HeLa lysate (35ug per lane, RIPA buffer) using STAM antibody (NB100-56533) at 1ug/ml. Band observed at ~65kDA. (Expected MW of 59.2kDa according to NP_003464.1).")
![Immunohistochemistry-Paraffin: Calmodulin Antibody (SR2721) [NBP3-27709] - Immunohistochemical analysis of paraffin-embedded human kidney](http://images.novusbio.com/fullsize/nbp3-27709_rabbit-calmodulin-mab-sr2721-75202410133411.jpg "Immunohistochemistry-Paraffin: Calmodulin Antibody (SR2721) [NBP3-27709] - Immunohistochemical analysis of paraffin-embedded human kidney")
![Western Blot: Calmodulin Antibody (SR2721) [NBP3-27709] - Western blot analysis of Calmodulin expression in (1) NIH-3T3 cell lysate; (2) MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27709_rabbit-calmodulin-mab-sr2721-752024936361.jpg)
![Western Blot: BCAR3 Antibody [NBP1-82523] - Analysis in human cell lines PC-3 and HEK293 using Anti-BCAR3 antibody. Corresponding BCAR3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Western-Blot-NBP1-82523-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: BCAR3 Antibody [NBP1-82523] - Analysis in human cell lines PC-3 and HEK293 using Anti-BCAR3 antibody. Corresponding BCAR3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BCAR3 Antibody [NBP1-82523] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and centrosome.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82523-img0014.jpg)
![Immunohistochemistry-Paraffin: BCAR3 Antibody [NBP1-82523] - Staining of human kidney shows moderate cytoplasmic positivity in tubular cells.](http://images.novusbio.com/fullsize/BCAR3-Antibody-Immunohistochemistry-Paraffin-NBP1-82523-img0013.jpg)
![STAM-1 antibody [HL1637] detects STAM-1 protein at endosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: STAM-1 stained by STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48660_rabbit-stam-1-mab-hl1637-immunocytochemistry-immunofluorescence-1411202518345919.jpg "STAM-1 antibody [HL1637] detects STAM-1 protein at endosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: STAM-1 stained by STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:500.Blue: Fluoroshield with DAPI .")
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48660_rabbit-stam-1-mab-hl1637-western-blot-141120251830222.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (10 | 10 | 20 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48660_rabbit-stam-1-mab-hl1637-western-blot-1411202518394113.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48660_rabbit-stam-1-mab-hl1637-western-blot-141120251841518.jpg)
![Immunocytochemistry/Immunofluorescence: AMSH/STAMBP Antibody [NBP1-90172] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AMSH-STAMBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90172-img0009.jpg "Immunocytochemistry/Immunofluorescence: AMSH/STAMBP Antibody [NBP1-90172] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: AMSH/STAMBP Antibody [NBP1-90172] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AMSH-STAMBP-Antibody-Immunohistochemistry-Paraffin-NBP1-90172-img0008.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-90172_rabbit-polyclonal-amsh-stambp-antibody-30520251074523.jpg)
![Simple Western:FBXO22 Antibody (OTI1H1)[NBP3-45022]](http://images.novusbio.com/fullsize/nbp3-45022_mouse-fbxo22-mab-oti1h1-western-blot-181202616161920.jpg "Simple Western:FBXO22 Antibody (OTI1H1)[NBP3-45022]")
![Simple Western:FBXO22 Antibody (OTI1H1)[NBP3-45022]](http://images.novusbio.com/fullsize/nbp3-45022_mouse-fbxo22-mab-oti1h1-western-blot-18120261618488.jpg)
![Simple Western:FBXO22 Antibody (OTI1H1)[NBP3-45022]](http://images.novusbio.com/fullsize/nbp3-45022_mouse-fbxo22-mab-oti1h1-western-blot-18120261616361.jpg)
![Simple Western:FBXO22 Antibody (OTI1H1)[NBP3-45022]](http://images.novusbio.com/fullsize/nbp3-45022_mouse-fbxo22-mab-oti1h1-simple-western-181202616171522.jpg)