| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44094 | |||
| BSA Free | NBP2-59027-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84827-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43911 | |||
| BSA Free | NBP2-52952-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89345-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38856-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72891 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06609SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33240 | |||
![Immunohistochemistry-Paraffin: CDK5RAP2 Antibody (CL3392) [NBP2-59027] - Staining of human kidney shows centrosome-like positivity in renal cells.](http://images.novusbio.com/fullsize/CDK5RAP2-Antibody-CL3392-Immunohistochemistry-Paraffin-NBP2-59027-img0008.jpg "Immunohistochemistry-Paraffin: CDK5RAP2 Antibody (CL3392) [NBP2-59027] - Staining of human kidney shows centrosome-like positivity in renal cells.")
![Immunohistochemistry-Paraffin: CDK5RAP2 Antibody (CL3392) [NBP2-59027] - Staining of human breast cancer shows centrosome-like immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/CDK5RAP2-Antibody-CL3392-Immunohistochemistry-Paraffin-NBP2-59027-img0005.jpg)
![Immunohistochemistry-Paraffin: CDK5RAP2 Antibody (CL3392) [NBP2-59027] - Staining of human testis shows centrosome-like immunoreactivity in a subset of seminiferous epithelium cells.](http://images.novusbio.com/fullsize/CDK5RAP2-Antibody-CL3392-Immunohistochemistry-Paraffin-NBP2-59027-img0007.jpg)
![Immunohistochemistry-Paraffin: CDK5RAP2 Antibody (CL3392) [NBP2-59027] - Staining of human tonsil shows centrosome-like positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CDK5RAP2-Antibody-CL3392-Immunohistochemistry-NBP2-59027-img0009.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84827 (A) shows similar protein distribution across tissues to independent antibody NBP1-84826 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0021.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0024.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0022.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0023.jpg)
![Western Blot: EGFR Antibody (CL0815) [NBP2-52952] - Lane 1: Marker [kDa], Lane 2: Human cell line A-431](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Western-Blot-NBP2-52952-img0004.jpg "Western Blot: EGFR Antibody (CL0815) [NBP2-52952] - Lane 1: Marker [kDa], Lane 2: Human cell line A-431")
![Immunohistochemistry-Paraffin: EGFR Antibody (CL0815) [NBP2-52952] - Staining of human ventricular cancer shows moderate membranous immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Immunohistochemistry-Paraffin-NBP2-52952-img0003.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (CL0815) [NBP2-52952] - Staining of human placenta shows strong membranous positivity in trophoblast.](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Immunohistochemistry-Paraffin-NBP2-52952-img0001.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (CL0815) [NBP2-52952] - Staining of human tonsil shows moderate membranous immunoreactivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Immunohistochemistry-Paraffin-NBP2-52952-img0002.jpg)
![Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining in human epididymis and testis tissues using anti-IFITM1 antibody. Corresponding IFITM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89345-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining in human epididymis and testis tissues using anti-IFITM1 antibody. Corresponding IFITM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: IFITM1 Antibody [NBP1-89345] - Analysis in human cell line CAPAN-2.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Western-Blot-NBP1-89345-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: IFITM1 Antibody [NBP1-89345] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89345-img0010.jpg)
![Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89345-img0017.jpg)
![Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human epididymis shows strong membranous positivity in ependymal cells.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89345-img0014.jpg)
![Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89345-img0015.jpg)
![Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human testis shows no membranous positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89345-img0016.jpg)
![Simple Western: IFITM1 Antibody [NBP1-89345] - Simple Western lane view shows a specific band for IFITM1 in 0.2 mg/ml of h. Tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IFITM1-Antibody-Simple-Western-NBP1-89345-img0006.jpg)
![Simple Western: IFITM1 Antibody [NBP1-89345] - Electropherogram image(s) of corresponding Simple Western lane view. IFITM1 antibody was used at 1:20 dilution on h. Tonsil lysate(s).](http://images.novusbio.com/fullsize/IFITM1-Antibody-Simple-Western-NBP1-89345-img0007.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining in human heart muscle and skin tissues.. Corresponding CDH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining in human heart muscle and skin tissues.. Corresponding CDH2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0009.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0006.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human skin shows no positivity in keratinocytes.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0007.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0008.jpg)
![Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] Analysis of extracts (35ug) from 9 different cell lines by using anti-N Cadherin monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Western-Blot-NBP2-01498-img0008.jpg "Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] Analysis of extracts (35ug) from 9 different cell lines by using anti-N Cadherin monoclonal antibody.")
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Human liver tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0007.jpg)
![Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-N Cadherin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Flow-Cytometry-NBP2-01498-img0002.jpg)
![Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY N Cadherin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-N Cadherin.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Western-Blot-NBP2-01498-img0003.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0004.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0005.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0006.jpg)
![Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - Analysis of Hela cells, using anti-N Cadherin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Flow-Cytometry-NBP2-01498-img0001.jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].](http://images.novusbio.com/fullsize/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06609-img0003.jpg "Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].")
![Western Blot: Nbs1 Antibody [NBP1-06609] - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody [NBP1-06609]. Observed molecular weight at ~99 kDa.](http://images.novusbio.com/fullsize/NBS1-Antibody-Western-Blot-NBP1-06609-img0004.jpg)
![Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609] - Immunohistochemical staining of MRE11, RAD50 & NBS1 proteins. Staining for each protein was scored as high or low as described in the Methods section. Representative examples of typical nuclear staining of MRE11 (a), RAD50 (b), & NBS1(c) scored as high expression in tumor cells. Correspondingly, examples of those scored as low expression for MRE11 (d), RAD50 (e), & NBS1 (f) are shown (40× magnification) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30176843), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06609_rabbit-polyclonal-nbs1-antibody-31020241517139.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Plasminogen antibody detects PLG protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7. 5 % SDS-PAGEPlasminogen antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0001.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0002.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0003.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0004.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0005.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Paraffin-embedded rat prostate Plasminogen antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ELF3/ESE-1 Antibody [NBP1-30873] - Methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30873-img0006.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-30873] - Human lung carcinoma. ESE1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30873-img0009.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-30873] - NCI-N87 xenograft, using ESE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30873-img0007.jpg)
![Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody [NBP1-30873] - The transcription factor ELF3 regulates the Grhl3 promoter. (A) The sequence of the Grhl3 promoter (1000 bp) showing the location of the predicted TATA box, SP1 sequence, & the transcription start site. Sequences in red represent the conserved regions, & green bases represent putative ELF3 binding sites. (B) Luciferase reporter assay showing the activation of the Grhl3 promoter by ELF3. Relative luciferase activity is calculated as fold induction relative to vector. Drawings on the left side show a schematic representation of the Grhl3 promoter region (top) & the structure of each reporter plasmid (below). The conserved regions are shown as horizontal boxes labeled 1,2 & 3, the restriction enzymes used to generate the reporter deletions of the promoter are labeled. (C) ELF3 binding at the promoter of Grhl3. NMuMG cells were treated with either vehicle or TGF beta 3 for 72 h, TGF beta 3 treated cells were then washed with PBS & continued incubation for an additional 48 h to initiate MET (post-treatment: PT48). Cells were cross-linked, & chromatin immunoprecipitation was performed. ChIP DNA was used in qPCR to measure the occupancy of ELF3 compared to control antibodies. (D) Sequence alignment of one putative ELF3 binding site in the Grhl3 promoter showing conservation among different species. In B & C, data represent the averages of at least three independent experiments. The paired Student’s t-test was used to calculate statistical significance. ** p-value < 0.01; **** p-value < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31404945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30873_rabbit-polyclonal-elf3-ese-1-antibody-310202416182629.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ELF3/ESE-1 Antibody [NBP1-30873] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ESE1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-30873_rabbit-polyclonal-elf3-ese-1-antibody-161020241941455.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse brain. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Immunohistochemistry-Paraffin-NBP2-15840-img0012.jpg)
![Western Blot: FZR1/CDH1 Antibody [NBP2-15840] - A431 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FZR1/CDH1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Western-Blot-NBP2-15840-img0013.jpg)
![Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse heart. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Immunohistochemistry-Paraffin-NBP2-15840-img0010.jpg)
![Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Rat heart. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Immunohistochemistry-Paraffin-NBP2-15840-img0011.jpg)
![Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Knockdown-Validated-NB100-56598-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Western-Blot-NB100-56598-img0005.jpg)
![Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Immunohistochemistry-NB100-56598-img0004.jpg)