| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52977SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44475 | |||
| BSA Free | NBP2-36760-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96907-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37378-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44476 | |||
| BSA Free | NBP2-36761-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44474 | |||
| BSA Free | NBP2-36759-25ul | |||
![Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Western-Blot-NBP2-80137-img0008.jpg "Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.")
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded human pancreas tissue usingTDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0007.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP-43/TARDBP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0009.jpg)
![Flow Cytometry: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of Hela cells with TDP-43/TARDBP Antibody (JM51-10) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Flow-Cytometry-NBP2-80137-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0006.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded mouse placenta tissue using TDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-52977] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Ezrin Antibody (NBP2-52977) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52977-img0005.jpg "Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-52977] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Ezrin Antibody (NBP2-52977) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-52977] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- conjugated to Alexa Fluor 647 (NBP2-52977AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52977-img0004.jpg)
![Western Blot: Ezrin Antibody [NBP2-52977] - Total protein from HeLa, Ntera2 and 3T3 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-EZR in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Western-Blot-NBP2-52977-img0003.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-52977] - IHC analysis of a formalin fixed paraffin embedded mouse kidney tissue section using rabbit anti-Ezrin antibody at 1:50 dilution. The sections were stained using HRP-DAB based detection followed by hematoxylin counterstaining. The antibody generated a membrane-cytoplasmic signal in the epithelial cells of various tubules and glomeruli did not show much of the expression.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunohistochemistry-Paraffin-NBP2-52977-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-52977] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EZR at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52977-img0001.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19438-img0009.jpg "Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0010.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19438) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19438_rabbit-polyclonal-mttfa-antibody-9102024205611.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.mtTFA stained by mtTFA antibody (NBP2-19438) diluted at 1:165.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19438_rabbit-polyclonal-mttfa-antibody-91020242014635.jpg)
![Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Western-Blot-NBP3-15677-img0007.jpg "Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded mouse brain using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0003.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded rat ovary using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0001.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded human esophageal using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0002.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315540.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of HeLa cells using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517352713.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunoprecipitation of TDP-43/TARDBP from 500 ug extracts of K-562 cells was performed using 2 ug of TDP-43/TARDBP Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X non-reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using TDP-43/TARDBP Rabbit mAb at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-5220251734597.jpg)
![Chromatin Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Chromatin immunoprecipitation analysis of extracts of K562 cells, using TDP-43/TARDBP Rabbit mAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517344289.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of paraffin-embedded Mouse brain tissue using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517363742.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517361227.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Human colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315511.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517311072.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat liver using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517304973.jpg)
![Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of extracts of various cell lines, using HMGA1 Rabbit mAb (NBP3-16379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HMGA1-Antibody-5J0E6-Western-Blot-NBP3-16379-img0005.jpg "Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of extracts of various cell lines, using HMGA1 Rabbit mAb (NBP3-16379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511383742.jpg)
![Chromatin Immunoprecipitation: HMGA1 Antibody (5J0E6) [NBP3-16379] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of HMGA1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of HMGA1 across chromosome 12(upper panel) and the genomic region encompassing HNF1A, a representative gene enriched in HMGA1 (lower panel).](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251141141.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374182.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251255541.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511402740.jpg)
![Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of lysates from Mouse testis, using HMGA1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251215443.jpg)
![Chromatin Immunoprecipitation: HMGA1 Antibody (5J0E6) [NBP3-16379] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of HMGA1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of HMGA1 in the representative genomic region surrounding HNF1A gene.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374168.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374132.jpg)
![Chromatin Immunoprecipitation: HMGA1 Antibody (5J0E6) [NBP3-16379] - Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using HMGA1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251215418.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511361253.jpg)
![Immunocytochemistry/ Immunofluorescence: HMGA1 Antibody (5J0E6) [NBP3-16379] - Confocal imaging of U-2 OS cells using HMGA1 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511411472.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-5220251215471.jpg)
![Immunohistochemistry: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using HMGA1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16379_rabbit-hmga1-mab-5j0e6-52202511374188.jpg)
![Western Blot: p38 alpha Antibody [NBP2-19662] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Western-Blot-NBP2-19662-img0010.jpg "Western Blot: p38 alpha Antibody [NBP2-19662] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded human lung adenocarcinoma. p38 antibody [N1C3-2] diluted at 1:250.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0003.jpg)
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded rat brain. p38 MAPK antibody [N1C3-2] diluted at 1:500.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0002.jpg)
![p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-immunocytochemistry-immunofluorescence-261120251995418.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-western-blot-261120251837242.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in human small intestine and skeletal muscle tissues. Corresponding EZR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunohistochemistry-Paraffin-NBP2-36760-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in human small intestine and skeletal muscle tissues. Corresponding EZR RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Ezrin Antibody (CL2384) [NBP2-36760] - Analysis of extracts from U-251 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-EZR monoclonal antibody.Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Western-Blot-NBP2-36760-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in U251 cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunocytochemistry-Immunofluorescence-NBP2-36760-img0011.jpg)
![Western Blot: Ezrin Antibody (CL2384) [NBP2-36760] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Western-Blot-NBP2-36760-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in HeLa cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunocytochemistry-Immunofluorescence-NBP2-36760-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human cell line A431 showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunocytochemistry-Immunofluorescence-NBP2-36760-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in MCF7 cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunocytochemistry-Immunofluorescence-NBP2-36760-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in U2OS cell line with Anti-EZR monoclonal antibody, showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunocytochemistry-Immunofluorescence-NBP2-36760-img0010.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human small intestine shows strong positivity in apical membranes in glandular cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunohistochemistry-Paraffin-NBP2-36760-img0013.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human placenta shows strong positivity in apical membrane in trophoblastic cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunohistochemistry-Paraffin-NBP2-36760-img0014.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunohistochemistry-Paraffin-NBP2-36760-img0015.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2384-Immunohistochemistry-Paraffin-NBP2-36760-img0016.jpg)
![Western Blot: p38 alpha Antibody [NB110-96907] - Western blot analysis of Human cancer cell lines showing detection of p38 alpha protein using Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Western-Blot-NB110-96907-img0008.jpg "Western Blot: p38 alpha Antibody [NB110-96907] - Western blot analysis of Human cancer cell lines showing detection of p38 alpha protein using Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody [NB110-96907] - Analysis using Rabbit Anti-p38 Polyclonal Antibody . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96907-img0007.jpg)
![Western Blot: p38 alpha Antibody [NB110-96907] - CtsK & NFATc1 expression levels were decreased during osteoclastogenesis by PSTP-3,5-Me treatment. (A) mRNA expression levels of osteoclast-specific markers were determined by RT-PCR in 0 or 6 µM PSTP-3,5-Me-treated cells. *p < 0.05, & **p < 0.01 indicate the statistically significant difference between non-PSTP-3,5-Me-treated groups (0 µM) & PSTP-3,5-Me-treated groups (6 µM) on each day. Mean ± standard error. (B) Western blotting was performed to determine phosphorylation of NF-kB, p-38, ERK1/2, & JNK. Cells were pre-treated with 6 µM PSTP-3,5-Me or vehicle (Ctrl) for 2 h, & then treated with RANKL for the indicated times. beta -Actin expression level was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31540026), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96907_rabbit-polyclonal-p38-alpha-antibody-31020241534327.jpg)
![Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NB100-58788-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Cullin 3 Antibody [NB100-58788] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunohistochemistry-NB100-58788-img0003.jpg)
![Western Blot: p38 alpha Antibody (8G4D11) [NBP2-37378] - Analysis using MAPK14 mouse mAb against HeLa (1), NIH/3T3 (2), Jurkat (3), Raw264.7 (4), PC-12 (5), C6 (6) and COS7 (7) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-8G4D11-Western-Blot-NBP2-37378-img0004.jpg "Western Blot: p38 alpha Antibody (8G4D11) [NBP2-37378] - Analysis using MAPK14 mouse mAb against HeLa (1), NIH/3T3 (2), Jurkat (3), Raw264.7 (4), PC-12 (5), C6 (6) and COS7 (7) cell lysate.")
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (8G4D11) [NBP2-37378] - Analysis of HeLa cells using MAPK14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/p38-Antibody-8G4D11-Immunocytochemistry-Immunofluorescence-NBP2-37378-img0005.jpg)
![Immunohistochemistry-Paraffin: p38 alpha Antibody (8G4D11) [NBP2-37378] - Analysis of cervical cancer tissues using MAPK14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p38-Antibody-8G4D11-Immunohistochemistry-NBP2-37378-img0009.jpg)
![Flow Cytometry: p38 alpha Antibody (8G4D11) [NBP2-37378] - Analysis of HeLa cells using MAPK14 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/p38-Antibody-8G4D11-Flow-Cytometry-NBP2-37378-img0006.jpg)
![Western Blot: p38 alpha Antibody (8G4D11) [NBP2-37378] - Analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 33 kDa)](http://images.novusbio.com/fullsize/p38-Antibody-8G4D11-Western-Blot-NBP2-37378-img0002.jpg)
![Western Blot: p38 alpha Antibody (8G4D11) [NBP2-37378] - Analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-8G4D11-Western-Blot-NBP2-37378-img0003.jpg)
![Immunohistochemistry-Paraffin: p38 alpha Antibody (8G4D11) [NBP2-37378] - Analysis of rectum cancer tissues using MAPK14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p38-Antibody-8G4D11-Immunohistochemistry-NBP2-37378-img0008.jpg)
![ELISA: p38 alpha Antibody (8G4D11) [NBP2-37378] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/p38-Antibody-8G4D11-ELISA-NBP2-37378-img0001.jpg)
![Western Blot: Ezrin Antibody (CL2360) [NBP2-36761] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2360-Western-Blot-NBP2-36761-img0007.jpg "Western Blot: Ezrin Antibody (CL2360) [NBP2-36761] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2360) [NBP2-36761] - Staining of U-251 cells using the Anti-EZR monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2360-Immunocytochemistry-Immunofluorescence-NBP2-36761-img0009.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2360) [NBP2-36761] - Staining of human skeletal muscle shows absence of immunoreactivity.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2360-Immunohistochemistry-NBP2-36761-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2360) [NBP2-36761] - Staining of A-431 cells using the Anti-EZR monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2360-Immunocytochemistry-Immunofluorescence-NBP2-36761-img0008.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2360) [NBP2-36761] - Staining of human colon shows strong membranous combined with moderate cytoplasmic immunoreactivity in glandular epithelium and lamina propria cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2360-Immunohistochemistry-NBP2-36761-img0001.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2360) [NBP2-36761] - Staining of human placenta shows strong membranous and moderate cytoplasmic positivity in the trophoblast.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2360-Immunohistochemistry-NBP2-36761-img0002.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2360) [NBP2-36761] - Staining of human prostate cancer shows strong membranous and moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2360-Immunohistochemistry-NBP2-36761-img0003.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2360) [NBP2-36761] - Staining of human stomach shows strong membranous combined with moderate cytoplasmic immunoreactivity in glandular epithelium and lamina propria cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2360-Immunohistochemistry-NBP2-36761-img0004.jpg)
![Western Blot: Ezrin Antibody (CL2375) [NBP2-36759] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2375-Western-Blot-NBP2-36759-img0006.jpg "Western Blot: Ezrin Antibody (CL2375) [NBP2-36759] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of A-431 cells using the Anti-EZR monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2375-Immunocytochemistry-Immunofluorescence-NBP2-36759-img0007.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human skeletal muscle shows absence of immunoreactivity.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2375-Immunohistochemistry-NBP2-36759-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of U-251 cells using the Anti-EZR monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2375-Immunocytochemistry-Immunofluorescence-NBP2-36759-img0008.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human small intestine shows strong membranous combined with moderate cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2375-Immunohistochemistry-NBP2-36759-img0001.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human colon shows strong membranous immunoreactivity in glandular epithelium and lamina propria cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2375-Immunohistochemistry-NBP2-36759-img0002.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human placenta shows strong membranous positivity in the trophoblast.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2375-Immunohistochemistry-NBP2-36759-img0003.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human stomach shows strong membranous combined with moderate cytoplasmic immunoreactivity in glandular epithelium and lamina propria cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2375-Immunohistochemistry-NBP2-36759-img0004.jpg)