| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25093-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37507-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-44995SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22118SS | |||
| Azide and BSA Free | NBP2-80587 | |||
![Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - KO Validation in 293T Cells Loading: 10 ug of lysate Antibodies: beta Amyloid, 0.5 ug/mL and beta-actin, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-2711202419333416.gif "<b>Genetic Strategies Validation. </b>Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - KO Validation in 293T Cells Loading: 10 ug of lysate Antibodies: beta Amyloid, 0.5 ug/mL and beta-actin, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunohistochemistry of beta Amyloid in human brain tissue with beta Amyloid antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120241918631.gif)
![Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunohistochemistry of beta Amyloid in human brain (Alzheimer's disease) tissue with beta Amyloid antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120242016992.jpg)
![Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 ug/mL.Green: beta Amyloid Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120241939277.gif)
![Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - Western blot analysis of beta Amyloid in (A) human, (B) mouse, and (C) rat brain tissue lysates with beta Amyloid antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120242022942.jpg)
![Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Western-Blot-NBP2-37507-img0003.jpg "Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of HeLa cells using CDK2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunocytochemistry-Immunofluorescence-NBP2-37507-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of colon cancer tissues using CDK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-NBP2-37507-img0007.jpg)
![Flow Cytometry: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of Jurkat cells using CDK2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Flow-Cytometry-NBP2-37507-img0005.jpg)
![Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mAb against human CDK2 (AA: 197-295) recombinant protein. (Expected MW is 36.8 kDa)](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Western-Blot-NBP2-37507-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of cervical cancer tissues using CDK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-NBP2-37507-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of CDK2 in colon cancer tissue using anti-CDK2 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-37507-img0008.jpg)
![ELISA: CDK2 Antibody (1A6) [NBP2-37507] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-ELISA-NBP2-37507-img0001.jpg)
![Western Blot: Folliculin Antibody [NBP1-44995] - Analysis of FLCN isoforms 1, 2 and 3 on HeLa whole cell lysate](http://images.novusbio.com/fullsize/Folliculin-Antibody-Western-Blot-NBP1-44995-img0004.jpg "Western Blot: Folliculin Antibody [NBP1-44995] - Analysis of FLCN isoforms 1, 2 and 3 on HeLa whole cell lysate")
![Immunocytochemistry/Immunofluorescence: Folliculin Antibody [NBP1-44995] - Detection of FLCN (Green) in Hela cells using NBP1-44995. Nuclei (Blue) are counterstained using Hoechst 33258](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44995-img0002.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. The SCC cells as well as the associated stromal cells depicted strong cytoplasmic-nuclear immunostaining of Folliculin protein. The staining was more intense in the carcinoma cells compared to the tumor stroma [Magnification 10X]](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0014.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. The carcinoma cells as well as the various stromal cells depicted cytoplasmic- nuclear immunostaining for Folliculin protein. The cytoplasmic staining was more intense in the carcinoma cells compared to tumor stroma [Magnification 40X]](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0010.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. Cytoplasmic-nuclear immunostaining of Folliculin protein was observed in the various cells and interestingly, the staining was intense and localized more towards the cell membranes in a bunch of cells having mesenchymal morphology. [Magnification 40X]](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0012.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal skin from human using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. Intense cytoplasmic with moderate nuclear immunostaining of Folliculin protein was observed in the various cells of the epidermal layer [Magnification 40X].](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0013.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal liver of human using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. Strong cytoplasmic-nuclear immunopositivity of Folliculin protein was observed in hepatocytes [Magnification 10X].](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0009.jpg)
![Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Western-Blot-NB100-2817-img0002.jpg "Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).")
![Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Immunohistochemistry-Paraffin-NB100-2817-img0004.jpg)
![Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Small Intestine. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Immunohistochemistry-Paraffin-NB100-2817-img0003.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Knockdown-Validated-H00005997-M01-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: RGS2 Antibody (4C4) [H00005997-M01] - Analysis of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Immunohistochemistry-Paraffin-H00005997-M01-img0005.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - RGS2 monoclonal antibody (M01), clone 4C4 Analysis of RGS2 expression in MCF-7.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0007.jpg)
![ELISA: RGS2 Antibody (4C4) [H00005997-M01] - Detection limit for recombinant GST tagged RGS2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-ELISA-H00005997-M01-img0008.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415394536.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) & Western blotting (WB). A. Detection by RPPA of VCL & ERK2 in serial dilution of HEK293 cells transfected with siRNA targeting VCL (siVCL) or non-silencing siRNA control (siCTR). B. Linear regression was performed for ERK2 & VCL protein & Pearson correlation between dilution & the signal intensity was calculated for each condition of Figure 3A, with all p-values lower than 0.001. Signal intensities are expressed as mean of the replicates. C. Level of expression by WB & RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB & spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415482256.jpg)
![Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Western-Blot-NBP2-22118-img0002.jpg "Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Immunocytochemistry-Immunofluorescence-NBP2-22118-img0004.jpg)
![Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Flow-Cytometry-NBP2-22118-img0003.jpg)
![ELISA: Aurora A Antibody (1F8) [NBP2-22118] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-ELISA-NBP2-22118-img0001.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0025.jpg "Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500")
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PCNA protein stained by PCNA antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0026.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0020.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug NIH-3T3 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0014.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse testis. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0013.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human colon. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0016.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human cervical carcinoma. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0017.jpg)
![Immunoprecipitation: PCNA Antibody [NBP1-32075] - Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 ug of PCBP2 antibody. Western blot analysis was performed using PCBP2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NBP1-32075-img0023.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (NBP1-32075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32075_rabbit-polyclonal-pcna-antibody-910202420131282.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19324] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Lamin A + C stained by Lamin A + C antibody diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19324-img0020.jpg "Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19324] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Lamin A + C stained by Lamin A + C antibody diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mouse stomach. Lamin A + C stained by Lamin A + C antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0022.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - C2C12 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - RT2 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Human breast carcinoma. Lamin A + C antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mahlarvu xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0018.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19324] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19324_rabbit-polyclonal-lamin-a-c-antibody-1610202419414527.jpg)
![Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.IP samples: HeLa whole cell extractA. 50 ug HeLa whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of Lamin A + C protein by 4 ug Lamin A + C antibody (NBP2-19324)7.5 % SDS-PAGEThe immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (NBP2-19324) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-19324_rabbit-polyclonal-lamin-a-c-antibody-immunoprecipitation-26112025199120.jpg)
![Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0046.jpg "<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-NB100-456-img0044.jpg)
![Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA . Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.](http://images.novusbio.com/fullsize/PCNA-Antibody-Flow-Cytometry-NB100-456-img0004.jpg)
![Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0041.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0043.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCNA Antibody [NB100-456] - Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody NB100-456 used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using another a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using NB100-456 (1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NB100-456-img0047.jpg)
![Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-NBP1-21367-img0006.jpg "Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367] - SUV39H1 expression in human glioma tissue grade IV. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Immunohistochemistry-Paraffin-NBP1-21367-img0003.jpg)
![Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up:SUV39H1 expression in normal brain, human glioma tissue grades II, III and IV respectively. Down: Actin expression levels. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Western-Blot-NBP1-21367-img0004.jpg)
![Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up: SUV39H1 expression in C6 astrocytic cell line and human glioma cell lines GOS-3, T98G, U87MG and 1321N1. Down: Actin levels. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Western-Blot-NBP1-21367-img0005.jpg)
![Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SASH1-Antibody-Western-Blot-NBP1-26650-img0004.jpg "Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate from HeLa cells. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody NBP1-26651.](http://images.novusbio.com/fullsize/SASH1-Antibody-Western-Blot-NBP1-26650-img0003.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0015.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Immunohistochemistry-Paraffin-NB100-594-img0016.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBEF antibody used for IP at 6 ug per reaction. PBEF was also immunoprecipitated by a previous lot of this antibody and rabbit anti-Visfatin antibody. For blotting immunoprecipitated PBEF, Antibody was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0014.jpg)