| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04398-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51596-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52580-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56394SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-439SS | |||
| Azide and BSA Free | NBP2-80945 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22832SS | |||
![Western Blot: KLHL9 Antibody [NBP3-04398] - Analysis of extracts of 293T cells, using KLHL9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .](http://images.novusbio.com/fullsize/KLHL9-Antibody-Western-Blot-NBP3-04398-img0005.jpg "Western Blot: KLHL9 Antibody [NBP3-04398] - Analysis of extracts of 293T cells, using KLHL9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .")
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded mouse testis using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0004.jpg)
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded rat ovary using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0001.jpg)
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded rat heart using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0002.jpg)
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded human breast cancer using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0003.jpg)
![Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Western-Blot-NBP1-51596-img0007.jpg "Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of NIH3T3 cells using SMC1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunocytochemistry-Immunofluorescence-NBP1-51596-img0010.jpg)
![Immunohistochemistry-Paraffin: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of paraffin-embedded human colon using SMC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunohistochemistry-Paraffin-NBP1-51596-img0008.jpg)
![Flow Cytometry: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of Hela cells using SMC1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Flow-Cytometry-NBP1-51596-img0009.jpg)
![Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mouse mAb against Hela (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Western-Blot-NBP2-52580-img0006.jpg "Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mouse mAb against Hela (1), and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis of paraffin-embedded esophageal cancer tissues using UBE2C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Immunohistochemistry-Paraffin-NBP2-52580-img0002.jpg)
![Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mAb against human UBE2C (AA: FULL(1-179)) recombinant protein. (Expected MW is 45.6 kDa)](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Western-Blot-NBP2-52580-img0003.jpg)
![Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mAb against HEK293 (1) and UBE2C (AA: FULL(1-179))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Western-Blot-NBP2-52580-img0004.jpg)
![Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis of paraffin-embedded liver cancer tissues using UBE2C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Immunohistochemistry-Paraffin-NBP2-52580-img0001.jpg)
![ELISA: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-ELISA-NBP2-52580-img0005.jpg)
![Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-278202415242619.jpg "Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32504) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-298202414262691.jpg)
![Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunocytochemistry analysis of Jurkat cells labeling KIFC1 with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202410334073.jpg)
![Flow Cytometry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Flow cytometric analysis of Jurkat cells labeling KIFC1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32504, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202416453263.jpg)
![Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-375-img0005.jpg "Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.")
![Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0002.jpg "Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.")
![Flow Cytometry: SMC4 Antibody [NB100-56394] - Western blot analysis of CAP-C in Ramos cell lysate using NB100-56394 at 2 ug/ml.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0001.jpg)
![Western Blot: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MIS12-Antibody-Western-Blot-NB100-2359-img0006.jpg "Western Blot: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MIS12 (NB100-2359). Detection: DAB](http://images.novusbio.com/fullsize/MIS12-Antibody-Immunohistochemistry-NB100-2359-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for IP at 3 ug per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody NB100-2358. For blotting immunoprecipitated MIS12, NB100-2359 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MIS12-Antibody-Immunoprecipitation-NB100-2359-img0005.jpg)
![Immunohistochemistry-Paraffin: SMC6L1 Antibody [NBP2-20420] - Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SMC6L1-Antibody-Immunohistochemistry-Paraffin-NBP2-20420-img0002.jpg "Immunohistochemistry-Paraffin: SMC6L1 Antibody [NBP2-20420] - Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using antibody at 1:500 dilution.")
![SMC6L1 antibody [N3C2], Internal detects SMC6 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SMC6 protein stained by SMC6L1 antibody [N3C2], Internal (NBP2-20420) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-20420_rabbit-polyclonal-smc6l1-antibody-immunocytochemistry-immunofluorescence-261120251819363.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC6L1 antibody [N3C2], Internal (NBP2-20420) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20420_rabbit-polyclonal-smc6l1-antibody-western-blot-261120251825266.jpg)
![SMC6L1 antibody [N3C2], Internal detects SMC6L1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.SMC6L1 stained by SMC6L1 antibody [N3C2], Internal (NBP2-20420) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20420_rabbit-polyclonal-smc6l1-antibody-immunohistochemistry-paraffin-261120251821104.jpg)
![Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.](http://images.novusbio.com/fullsize/Separase-Antibody-XJ11-1B12-Western-Blot-NB100-439-img0002.jpg "Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.")
![Western Blot: MAD1L1/MAD1 Antibody [NB100-569] - Detection of Human MAD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody NB100-569 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NB100-569-img0014.jpg "Western Blot: MAD1L1/MAD1 Antibody [NB100-569] - Detection of Human MAD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody NB100-569 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NB100-569] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunohistochemistry-NB100-569-img0013.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: MAD1L1/MAD1 Antibody [NB100-569] - Whole cell lysate (50 mcg/lane in A; 50 mcg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). NB100-569 used at 0.1 mcg/ml for Western Blot (A) and at 1 mcg/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies NB100-570 and NB100-571 using 1 mcg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using NB100-570 at 0.2 mcg/ml.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunoprecipitation-NB100-569-img0009.jpg)
![Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-22832-img0004.jpg "Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.")
![Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - A:NIH-3T3.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Western-Blot-NBP2-20782-img0004.jpg "Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - A:NIH-3T3.")
![Western Blot: UbcH10/UBE2C Antibody [NBP2-20782] - Expression in transfected 293T cell line by UBE2C polyclonal antibody. A: Non-transfected lysate. B: UBE2C transfected lysate.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Western-Blot-NBP2-20782-img0005.jpg)
![UBE2C antibody [N1C3] detects UBE2C protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: UBE2C protein stained by UBE2C antibody [N1C3] (NBP2-20782) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20782_rabbit-polyclonal-ubch10-ube2c-antibody-immunocytochemistry-immunofluorescence-261120251831282.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with UBE2C antibody [N1C3] (NBP2-20782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20782_rabbit-polyclonal-ubch10-ube2c-antibody-western-blot-2611202518395717.jpg)