| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71242 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-41050-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72294 | |||
![Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Western-Blot-NBP2-76416-img0002.jpg "Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunocytochemistry-NBP2-76416-img0001.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) and rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunoprecipitation-NBP2-76416-img0003.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76416_rabbit-monoclonal-b7-2-cd86-antibody-blr029f-immunoprecipitation-13120251501..jpg)
![Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - Staining of HeLa cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Western-Blot-NB100-834-img0004.jpg "Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - Staining of HeLa cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Immunohistochemistry-Paraffin-NB100-834-img0006.jpg)
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Immunohistochemistry-Paraffin-NB100-834-img0005.jpg)
![Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP1-77894-img0002.jpg "Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.")
![Immunohistochemistry: IL-6 Antibody [NBP1-77894] - Shows nuclear positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunohistochemistry-NBP1-77894-img0004.jpg)
![Flow Cytometry: Axl Antibody (MM0098-2N33) [NBP2-12044] - A cell surface stain was performed on U87MG cells with Axl antibody (MM0098-2N33) NBP2-12044AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Axl-Antibody-MM0098-2N33-Flow-Cytometry-NBP2-12044-img0004.jpg "Flow Cytometry: Axl Antibody (MM0098-2N33) [NBP2-12044] - A cell surface stain was performed on U87MG cells with Axl antibody (MM0098-2N33) NBP2-12044AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow (Cell Surface): Axl Antibody (MM0098-2N33) [NBP2-12044] - A cell surface stain was performed on HeLa cells with AXL antibody (MM0098-2N33) NBP2-12044PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Axl-Antibody-MM0098-2N33-Flow-Cell-Surface-NBP2-12044-img0002.jpg)
![Flow Cytometry: Axl Antibody (MM0098-2N33) [NBP2-12044] - Using the Alexa Fluor 647 direct conjugate A cell surface stain was performed on HeLa cells with AXL antibody (MM0098-2N33) NBP2-12044AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Axl-Antibody-MM0098-2N33-Flow-Cytometry-NBP2-12044-img0001.jpg)
![Flow Cytometry: Axl Antibody (MM0098-2N33) [NBP2-12044] - A cell surface stain was performed on HeLa cells with AXL antibody (MM0098-2N33) NBP2-12044AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Axl-Antibody-MM0098-2N33-Flow-Cytometry-NBP2-12044-img0003.jpg)
![Western Blot: ARHGEF1 Antibody (4C4) [H00009138-M03] - Analysis of ARHGEF1 expression in K-562 (Cat # L009V1).](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-4C4-Western-Blot-H00009138-M03-img0005.jpg "Western Blot: ARHGEF1 Antibody (4C4) [H00009138-M03] - Analysis of ARHGEF1 expression in K-562 (Cat # L009V1).")
![Immunocytochemistry/Immunofluorescence: ARHGEF1 Antibody (4C4) [H00009138-M03] - Analysis of monoclonal antibody to ARHGEF1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-4C4-Immunocytochemistry-Immunofluorescence-H00009138-M03-img0004.jpg)
![ELISA: ARHGEF1 Antibody (4C4) [H00009138-M03] - Detection limit for recombinant GST tagged ARHGEF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-4C4-ELISA-H00009138-M03-img0003.jpg)
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - HSP27 (phospho Ser82) antibody [GT357] detects HSP27 (phospho Ser82) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP27 (phospho Ser82) stained by HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Immunocytochemistry-Immunofluorescence-NBP3-13617-img0002.jpg)
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0003.jpg)
![Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13617_mouse-hsp27-p-ser-82-mab-gt357-western-blot-2611202518484816.jpg)
![Western Blot: PBK Antibody [NBP3-12989] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:60000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PBK-Antibody-Western-Blot-NBP3-12989-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PBK Antibody [NBP3-12989] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:60000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: PBK Antibody [NBP3-12989] - PBK antibody detects PBK protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:1000.](http://images.novusbio.com/fullsize/PBK-Antibody-Western-Blot-NBP3-12989-img0001.jpg)
![Western Blot: PBK Antibody [NBP3-12989] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:1000.](http://images.novusbio.com/fullsize/PBK-Antibody-Western-Blot-NBP3-12989-img0004.jpg)
![Immunoprecipitation: PBK Antibody [NBP3-12989] - Immunoprecipitation of PBK protein from A431 whole cell extracts using 5 ug of PBK antibody (NBP3-12989). Western blot analysis was performed using PBK antibody (NBP3-12989) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PBK-Antibody-Immunoprecipitation-NBP3-12989-img0002.jpg)
![Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Western-Blot-NBP2-45915-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0003.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0004.jpg)
![Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Western-Blot-NBP1-47854-img0006.jpg "Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunocytochemistry-Immunofluorescence-NBP1-47854-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunohistochemistry-Paraffin-NBP1-47854-img0005.jpg)
![Immunohistochemistry-Paraffin: SP-D Antibody (1.7) [NBP1-41050] - Staining of frozen pig lung with Mouse anti pig Surfactant Protein D](http://images.novusbio.com/fullsize/SP-D-Antibody-1-7-Immunohistochemistry-Paraffin-NBP1-41050-img0002.jpg "Immunohistochemistry-Paraffin: SP-D Antibody (1.7) [NBP1-41050] - Staining of frozen pig lung with Mouse anti pig Surfactant Protein D")
![Western Blot: ECRG4 Antibody (3B10) [NBP2-00599] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECRG4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECRG4.](http://images.novusbio.com/fullsize/ECRG4-Antibody-3B10-Western-Blot-NBP2-00599-img0008.jpg "Western Blot: ECRG4 Antibody (3B10) [NBP2-00599] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECRG4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECRG4.")
![Immunocytochemistry/Immunofluorescence: ECRG4 Antibody (3B10) [NBP2-00599] - Immunofluorescent staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ECRG4-Antibody-3B10-Immunocytochemistry-Immunofluorescence-NBP2-00599-img0007.jpg)
![Flow Cytometry: ECRG4 Antibody (3B10) [NBP2-00599] - Analysis of Jurkat cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ECRG4-Antibody-3B10-Flow-Cytometry-NBP2-00599-img0006.jpg)
![Flow Cytometry: ECRG4 Antibody (3B10) [NBP2-00599] - Analysis of Hela cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ECRG4-Antibody-3B10-Flow-Cytometry-NBP2-00599-img0005.jpg)