| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-386SS | |||
| Azide and BSA Free | NBP2-80922 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84573-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78133SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04585-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56491SS | |||
![Western Blot: HSPA2 Antibody [NBP2-16897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/HSPA2-Antibody-Western-Blot-NBP2-16897-img0007.jpg "Western Blot: HSPA2 Antibody [NBP2-16897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.")
![Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP2-16897] - HSPA2 antibody detects HSPA2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HSPA2 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HSPA2-Antibody-Immunohistochemistry-Paraffin-NBP2-16897-img0005.jpg)
![Western Blot: HspA2 Antibody [NBP2-16897] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/HspA2-Antibody-Western-Blot-NBP2-16897-img0001.jpg)
![Western Blot: HspA2 Antibody [NBP2-16897] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/HspA2-Antibody-Western-Blot-NBP2-16897-img0002.jpg)
![Western Blot: HSPA2 Antibody [NBP2-16897] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/HSPA2-Antibody-Western-Blot-NBP2-16897-img0004.jpg)
![Western Blot: HSPA2 Antibody [NBP2-16897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/HSPA2-Antibody-Western-Blot-NBP2-16897-img0006.jpg)
![Western Blot: Rad21 Antibody (CM110-2C10) [NB100-386] - Western blot with monoclonal anti-Rad21 (NB100-386) in HEK293 lysates.](http://images.novusbio.com/fullsize/Rad21-Antibody-CM110-2C10-Western-Blot-NB100-386-img0003.jpg "Western Blot: Rad21 Antibody (CM110-2C10) [NB100-386] - Western blot with monoclonal anti-Rad21 (NB100-386) in HEK293 lysates.")
![Simple Western: Rad21 Antibody (CM110-2C10) [NB100-386] - Simple Western lane view shows a specific band for Rad21 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Rad21-Antibody-CM110-2C10-Simple-Western-NB100-386-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cdc14A Antibody [NBP1-84573] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cdc14A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84573-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cdc14A Antibody [NBP1-84573] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.")
![Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Western-Blot-NBP2-78133-img0002.jpg "Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NBP2-78133] - IHC analysis of a FFPE tissue section of the mouse testis using 1:200 dilution of SCP3 antibody (NBP2-78133). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated strong staining associated with chromatin in meiotic cells.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-Paraffin-NBP2-78133-img0001.jpg)
![Western Blot: TPX2 Antibody (18D5-1) [NB100-74556] - Analysis of TPX2.](http://images.novusbio.com/fullsize/TPX2-Antibody-18D5-1-Western-Blot-NB100-74556-img0002.jpg "Western Blot: TPX2 Antibody (18D5-1) [NB100-74556] - Analysis of TPX2.")
![Immunohistochemistry-Paraffin: TPX2 Antibody (18D5-1) [NB100-74556] - Biopsies of deparaffinized Human testis tissue.](http://images.novusbio.com/fullsize/TPX2-Antibody-18D5-1-Immunohistochemistry-Paraffin-NB100-74556-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-10122024140443.jpg "Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177017.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177023.jpg)
![Western Blot: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Western Blot analysis of various lysates using ERK1/2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-31120251744429.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025173186.jpg)
![Western Blot: KIFC1 Antibody [NBP3-04585] - Analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Western-Blot-NBP3-04585-img0005.jpg "Western Blot: KIFC1 Antibody [NBP3-04585] - Analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody [NBP3-04585] - Analysis of U-2 OS cells using KIFC1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04585-img0001.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded mouse testis using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0004.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded rat testis using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0002.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded human colon carcinoma using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0003.jpg)
![Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-288202412442960.jpg "Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32753) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-298202414161863.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1 with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202410334043.jpg)
![Flow Cytometry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Flow cytometric analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32753, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202416372140.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0004.jpg "Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.")
![Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Immunohistochemistry-Paraffin-NBP2-67555-img0005.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on A431 lysates using anti-Erk1(pT202/pY204)+Erk2(pT185/pY187) antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0001.jpg)
![ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1 (pT202/pY204) + Erk2 (pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with lambda Protein Phosphatase whole cell lysates](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-NBP2-67555-img0003.jpg)
![Western Blot: DUSP13 Antibody [NB100-56491] - Analysis of DUSP13 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/DUSP13-Antibody-Western-Blot-NB100-56491-img0002.jpg "Western Blot: DUSP13 Antibody [NB100-56491] - Analysis of DUSP13 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 5 ug/ml.")
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0005.jpg "Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.")
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0006.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0002.jpg)
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0007.jpg)
![Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0021.jpg "Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PLK1 (NB100-547). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-547-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 on HeLa whole cell lysate using NB100-547. B. Affinity purified rabbit anti-PLK1 antibodies NB100-546, NB100-547, and NB100-548. IPed PLK1 was detected using NB100-548.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0020.jpg)