| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92361-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85731-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44244 | |||
| BSA Free | NBP2-61431-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85217-25ul | |||
![Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution were used for this test](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NBP2-27181-img0004.jpg "Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution were used for this test")
![Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human adrenal tissue stained with NUMB antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunohistochemistry-Paraffin-NBP2-27181-img0006.jpg)
![Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human spleen stained with NUMB antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunohistochemistry-Paraffin-NBP2-27181-img0005.jpg)
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NB100-79807-img0011.jpg "Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Detection of human RCH1Importin alpha 2/KPNA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCH1 antibody NB100-79807 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCH1 was also immunoprecipitated by a previous lot of NB100-79807 (lot 1) and by rabbit anti-RCH1 antibody NB100-79808, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunoprecipitation-NB100-79807-img0012.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19323-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human breast carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0012.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Lamin A/C antibody detects LMNA protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lamin A/C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse prostate. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse fore brain. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0008.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse skin. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human endometrial carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0011.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-418_rabbit-polyclonal-neurofibromin-1-antibody-western-blot-132202618215117.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92100_rabbit-polyclonal-mat2a-antibody-western-blot-13220261739820.jpg)
![Western Blot: p38 alpha Antibody [NBP2-19662] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Western-Blot-NBP2-19662-img0010.jpg "Western Blot: p38 alpha Antibody [NBP2-19662] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded human lung adenocarcinoma. p38 antibody [N1C3-2] diluted at 1:250.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0003.jpg)
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded rat brain. p38 MAPK antibody [N1C3-2] diluted at 1:500.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0002.jpg)
![p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-immunocytochemistry-immunofluorescence-261120251995418.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-western-blot-261120251837242.jpg)
![Western Blot: SHC1 Antibody (3F4) [H00006464-M01] - SHC1 monoclonal antibody (M01), clone 3F4 Analysis of SHC1 expression in A-431.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Western-Blot-H00006464-M01-img0015.jpg "Western Blot: SHC1 Antibody (3F4) [H00006464-M01] - SHC1 monoclonal antibody (M01), clone 3F4 Analysis of SHC1 expression in A-431.")
![Immunocytochemistry/Immunofluorescence: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of monoclonal antibody to SHC1 on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Immunocytochemistry-Immunofluorescence-H00006464-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of monoclonal antibody to SHC1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Immunohistochemistry-Paraffin-H00006464-M01-img0013.jpg)
![Proximity Ligation Assay: SHC1 Antibody (3F4) [H00006464-M01] - Protein-protein interactions between STAT5A and SHC1 Mahlavu cells were stained with anti-STAT5A rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Proximity-Ligation-Assay-H00006464-M01-img0007.jpg)
![Proximity Ligation Assay: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of protein-protein interactions between PIK3R1 and SHC1. HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Proximity-Ligation-Assay-H00006464-M01-img0008.jpg)
![Proximity Ligation Assay: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of protein-protein interactions between PIK3R1 and SHC1. Huh7 cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Proximity-Ligation-Assay-H00006464-M01-img0009.jpg)
![ELISA: SHC1 Antibody (3F4) [H00006464-M01] - Detection limit for recombinant GST tagged SHC1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-ELISA-H00006464-M01-img0016.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0016.jpg "Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of monoclonal antibody to NME2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Immunocytochemistry-Immunofluorescence-H00004831-M06-img0009.jpg)
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of monoclonal antibody to NME2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Immunohistochemistry-Paraffin-H00004831-M06-img0011.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0013.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0014.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0015.jpg)
![Sandwich ELISA: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Detection limit for recombinant GST tagged NME2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Sandwich-ELISA-H00004831-M06-img0012.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining in human esophagus and pancreas tissues . Corresponding S100A16 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining in human esophagus and pancreas tissues . Corresponding S100A16 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: S100A16 Antibody [NBP1-92361] - Analysis in SK-BR-3 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-S100A16 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/S100A16-Antibody-Western-Blot-NBP1-92361-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: S100A16 Antibody [NBP1-92361] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92361-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100A16 Antibody [NBP1-92361] - Analysis in human cell lines A-431 and HEK293 using Anti-S100A16 antibody. Corresponding S100A16 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/S100A16-Antibody-Western-Blot-NBP1-92361-img0025.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of glial cells.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0018.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining of human esophagus shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0019.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0020.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining of human skin shows moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0021.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human cerebral cortex, lymph node, skeletal muscle and testis using Anti-SEPT7 antibody NBP1-85731 (A) shows similar protein distribution across tissues to independent antibody NBP1-85730 (B).](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-7 Antibody [NBP1-85731] - Staining of human cell line A-431 shows localization to nucleoli, plasma membrane & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85731-img0012.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0024.jpg)
![Western Blot: Septin-7 Antibody [NBP1-85731] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Western-Blot-NBP1-85731-img0018.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human testis shows moderate positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0021.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0022.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0023.jpg)
![Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Septin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-85731-img0025.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Western-Blot-NBP2-61431-img0010.jpg "Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of A549 cells. Nuclear probes are visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunocytochemistry-Immunofluorescence-NBP2-61431-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0008.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Western-Blot-NBP2-61431-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of SH-SY5Y cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunocytochemistry-Immunofluorescence-NBP2-61431-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human cerebellum shows strong positivity in both neuronal cell bodies and neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human prostate shows strong positivity in the peripheral nerves.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0007.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: DIAPH-2 Antibody [NBP1-85217] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NBP1-85217-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: DIAPH-2 Antibody [NBP1-85217] - Staining of human cell line U-251 MG shows localization to nucleoli, endoplasmic reticulum and vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85217-img0016.jpg)
![Western Blot: DIAPH-2 Antibody [NBP1-85217] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NBP1-85217-img0007.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0013.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0014.jpg)