| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43347-0.5mg | |||
| BSA Free | NBP1-43347-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70891 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56462SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44228 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04982-0.025ml | |||
![Western Blot: VE-Cadherin Antibody (BV14) [NBP1-43347] - Non-reduced (left) and reduced (right) bEnd.3 cell line lysates were loaded at 1x10^5 cells/lane, probed with 2 ug/mL of Anti-Mouse CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV14-Western-Blot-NBP1-43347-img0003.jpg "Western Blot: VE-Cadherin Antibody (BV14) [NBP1-43347] - Non-reduced (left) and reduced (right) bEnd.3 cell line lysates were loaded at 1x10^5 cells/lane, probed with 2 ug/mL of Anti-Mouse CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.")
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing expression of CCAR1 and necdin in primary myoblasts isolated from wt, Ndn-/-, tgMlcNec newborn mice. Proteins were detected using antibodies specific for CCAR1, necdin and beta-actin as control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0010.jpg "Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing expression of CCAR1 and necdin in primary myoblasts isolated from wt, Ndn-/-, tgMlcNec newborn mice. Proteins were detected using antibodies specific for CCAR1, necdin and beta-actin as control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: CCAR1 Antibody [NB500-186] - Necdin interacts with CCAR1/CARP1. Co-localization of necdin and CCAR1 in primary myoblasts of wt (C57/Bl6) mice. Images taken at confocal laser scanning microscope at 40x (i-v) and 63x (zoomed 1.67 times) magnification. Nuclei are stained with DAPI (i, vi; blue). Panels shows immunostaining experiment using the polyclonal anti-CCAR1 (ii, vii, Ab-CCAR1-red) or monoclonal anti-Ndn (iii, viii; Ab-Ndn-green). Panel iv shows the merged images of ii and iii and panel ix the merged images of vii and viii (Ab-CCAR1 + Ab-Ndn-yellow). Panel v shows the merged images of i, ii and iii and panel x the merged images of vi, vii, viii, (Ab-CCAR + Ab-Ndn + DAPI). Scale bar (i-iv) = 17 um; (vi-x) = 41,75 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB500-186-img0011.jpg)
![Immunohistochemistry-Paraffin: CCAR1 Antibody [NB500-186] - Human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-CCAR1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunohistochemistry-Paraffin-NB500-186-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CCAR1 Antibody [NB500-186] - Detection of Human CCAR1on HeLa whole cell lysate using NB500-186. CCAR1 was also IPed with another rabbit anti-CCAR1 antibody, NB500-187, and NB500-188. IPed CCAR1 was blotted using NB500-188.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CCAR1 Antibody [NB500-186] - Detection of Human and Mouse CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0006.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin interacts with CCAR1/CARP1. Interaction of necdin and CCAR1 in C2C12 myoblasts and primary myoblast cells. Co-IP experiments were performed on protein extract from C2C12 transfected with pCMV-FLAG-Necdin (C2C12 pCMV-FLAG-Ndn) or primary myoblasts from C57/Bl6 newborn mice. Proteins were immunoprecipitated using the polyclonal anti-Ndn (alpha-Ndn) or polyclonal anti-CCAR1 (alpha-CCAR1) and with a non-specific rabbit antisera as control (IgG). Necdin and CCAR1 were detected in immunoprecipitated samples using specific antibody. Input (I) represents 10% of the immunoprecipitated proteins. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0008.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing CCAR1 and necdin (beta-actin as loading control) expression in C2C12 mock transfected (M) or transfected with pSG5-HA-CCAR1 and/or pCMV-FLAG-Necdin (N or N+C). Both the endogenous and transfected CCAR1 were detected with the polyclonal anti-CCAR1, the transfected CCAR1 with anti-HA. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CCAR1 Antibody [NB500-186] - Images taken at confocal laser scanning microscope (63x magnif. zoomed 1.67 x) showing co-immunostaining on C2C12 transfected with pSG5-HA-CCAR1 (i) and/or pCMV-Ndn (C2C12, CCAR1-Ndn-N+C) (ii, iii) and on primary myoblasts from Ndn-/- (Ndn-/-) (iv) and tgMlcNec (MlcNec) (v) newborn mice. Panels i-v show immunostaining using the specific monoclonal anti-Ndn (Ndn: Ab-Ndn-green); panels i'-v' show immunostaining using the polyclonal anti-CCAR1 (CCAR1: Ab-CCAR1-red). Co-immunostained images of anti-Ndn and anti-CCAR1 are shown in panels i''-v'' (Ab-CCAR1 + Ab-Ndn-yellow). Panels i'''-v''' show merged images with nuclei stained with DAPI (merge Ab-CCAR1 + ab-Ndn + DAPI). Scale bars (i-iii) 41,75 um; (iv-v) 44,7 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB500-186-img0012.jpg)
![Immunoprecipitation: CCAR1 Antibody [NB500-186] - Detection of human CCAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-186 (lot NB500-186-2) used for IP at 6 ug/mg lysate. CCAR1 was also immunoprecipitated by a previous lot (lot NB500-186-1) of the antibody. For blotting immunoprecipitated CCAR1, NB500-186 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunoprecipitation-NB500-186-img0007.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Autoantibody discovery in anti–TIF1-gamma –positive DM patients without cancer.(A) Immunoprecipitations (IPs) were performed using lysates made from radiolabeled cells & plasma from anti–TIF1-gamma –positive DM patients, 5 of whom did not have a cancer, & 5 of whom had a detected cancer. An IP performed with a sample from a healthy control (HC) individual is shown in the right-most lane. Migration of molecular weight standards is marked on the left. (B) Immunoblotted lysates. Lysates made from HeLa & A431 cells were immunoblotted with commercial antibodies against TIF1-gamma & CCAR1, as described in the Methods section. Both proteins migrate at approximately 150 kDa. (C) Interaction between CCAR1 & TIF1-gamma . Co-IPs were performed as described in the Methods section, using antibodies against CCAR1 (upper panel, 2 left lanes) or TIF1-gamma (lower panel, 2 left lanes). Detection of the IPs was performed by immunoblotting with anti–TIF1-gamma (upper panel, 2 left lanes) or anti-CCAR1 (lower panel, 2 left lanes) antibodies. Control IPs, performed using Protein A beads only, were performed & immunoblotted as above. IPs were performed in duplicate. These data are representative of those obtained in 2 additional experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35040440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-186_rabbit-polyclonal-ccar1-antibody-310202416182617.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Autoantibody discovery in anti–TIF1-gamma –positive DM patients without cancer.(A) Immunoprecipitations (IPs) were performed using lysates made from radiolabeled cells & plasma from anti–TIF1-gamma –positive DM patients, 5 of whom did not have a cancer, & 5 of whom had a detected cancer. An IP performed with a sample from a healthy control (HC) individual is shown in the right-most lane. Migration of molecular weight standards is marked on the left. (B) Immunoblotted lysates. Lysates made from HeLa & A431 cells were immunoblotted with commercial antibodies against TIF1-gamma & CCAR1, as described in the Methods section. Both proteins migrate at approximately 150 kDa. (C) Interaction between CCAR1 & TIF1-gamma . Co-IPs were performed as described in the Methods section, using antibodies against CCAR1 (upper panel, 2 left lanes) or TIF1-gamma (lower panel, 2 left lanes). Detection of the IPs was performed by immunoblotting with anti–TIF1-gamma (upper panel, 2 left lanes) or anti-CCAR1 (lower panel, 2 left lanes) antibodies. Control IPs, performed using Protein A beads only, were performed & immunoblotted as above. IPs were performed in duplicate. These data are representative of those obtained in 2 additional experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35040440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-186_rabbit-polyclonal-ccar1-antibody-310202416165639.jpg)
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n = 3) (GLUT4 - NBP1-49533; HK2/Hexokinase 2 - NBP2-02272). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0021.jpg "Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n = 3) (GLUT4 - NBP1-49533; HK2/Hexokinase 2 - NBP2-02272). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hexokinase 2.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Immunocytochemistry-Immunofluorescence-NBP2-02272-img0012.jpg)
![Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells transfected with either pCMV6-ENTRY Hexokinase 2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Hexokinase 2 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Flow-Cytometry-NBP2-02272-img0019.jpg)
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hexokinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hexokinase 2.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0017.jpg)
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hexokinase 2I monoclonal antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of HepG2 cells using anti-Hexokinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Immunocytochemistry-Immunofluorescence-NBP2-02272-img0020.jpg)
![Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Western-Blot-NB100-56462-img0004.jpg "Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: Smad2 Antibody [NB100-56462] - Mouse testis tissue probed with this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Immunohistochemistry-Paraffin-NB100-56462-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV9-Immunocytochemistry-Immunofluorescence-NB600-1409-img0008.jpg "Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.")
![Flow Cytometry: VE Cadherin Antibody (BV9) [NB600-1409] - HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4 degrees C.(Black- no stain, Red- isotype control, Blue-HM2032-BV9)](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV9-Flow-Cytometry-NB600-1409-img0007.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Western-Blot-NBP1-51643-img0010.jpg "Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.")
![Immunohistochemistry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Images of IHC analysis of B7-H3 and HK2 protein expression and hematoxylin and eosin (H&E) staining of CRC (n=126) tissue sections. One representative image is shown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-019-1549-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-NBP1-51643-img0012.jpg)
![Flow Cytometry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Flow-Cytometry-NBP1-51643-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-Paraffin-NBP1-51643-img0009.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 promoted the expression of HK2 in CRC cells.a, b The expression of glycolysis-related genes was detected by RT-qPCR in both B7-H3-overexpressing HCT116 (a) & RKO (b) cells. c, d The mRNA level of HK2 was detected by RT-qPCR in both HCT116 (c) & RKO (d) cells after transfection with siRNA NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. e HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both HCT116 & RKO cells after transfection with NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. beta -actin served as a loading control. f HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both B7-H3-overexpressing HCT116 & RKO cells. beta -actin served as a loading control. g Schematic representation of the proposed B7-H3/STAT3/HK2 axis. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202416173538.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 regulated glycolysis through HK2.a The protein level of HK2 in B7-H3-overexpressing HCT116 or RKO cells after transfection with siRNA negative control (NC) or HK2 siRNA transfection were analyzed by western blot. beta -actin served as a loading control. b, c Glucose consumption (b) & lactate production (c) were measured in B7-H3-overexpressing HCT116 or RKO cells transfected with NC or HK2 siRNA. d, e Glucose consumption (d) & lactate production (e) were measured in B7-H3-overexpressing HCT116 or RKO cells treated with PBS or 2-DG. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202415175275.jpg)
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16814-img0017.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0022.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - U87 xenograft. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-II-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0003.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0005.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat heart. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat stomach. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0008.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat colon. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0012.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat kidney. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0013.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse heart. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0014.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0015.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human fallopian tube and pancreas tissues using NBP2-88916 antibody. Corresponding SMAD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human fallopian tube and pancreas tissues using NBP2-88916 antibody. Corresponding SMAD2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human cell line U-138MG.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Western-Blot-NBP2-88916-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of HeLa cells using the Anti-SMAD2 monoclonal antibody, showing specific staining in the cytosol and nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunocytochemistry-Immunofluorescence-NBP2-88916-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human breast cancer shows moderate cytoplasmic positivity.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human Fallopian tube shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human pancreas shows low cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0004.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human testis shows weak to moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0006.jpg)
![Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Western-Blot-NBP2-37580-img0003.jpg "Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (5G7) [NBP2-37580] - Immunofluorescence analysis of U251 cells using SMAD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Immunocytochemistry-Immunofluorescence-NBP2-37580-img0004.jpg)
![Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using SMAD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Immunohistochemistry-NBP2-37580-img0007.jpg)
![Flow Cytometry: Smad2 Antibody (5G7) [NBP2-37580] - Flow cytometric analysis of NIH/3T3 cells using SMAD2 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Flow-Cytometry-NBP2-37580-img0005.jpg)
![Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against human SMAD2 (AA: 20-254) recombinant protein. (Expected MW is 52.2 kDa)](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Western-Blot-NBP2-37580-img0002.jpg)
![Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using SMAD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Immunohistochemistry-NBP2-37580-img0006.jpg)
![ELISA: Smad2 Antibody (5G7) [NBP2-37580] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-ELISA-NBP2-37580-img0001.jpg)
![Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in A-431.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-4H1-Western-Blot-H00003099-M01-img0007.jpg "Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in A-431.")
![Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in PC-12.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-4H1-Western-Blot-H00003099-M01-img0006.jpg)
![Sandwich ELISA: Hexokinase 2 Antibody (4H1) [H00003099-M01] - Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-4H1-Sandwich-ELISA-H00003099-M01-img0005.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0004.jpg "Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad2 Rabbit mAb (NBP3-15671) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunocytochemistry-Immunofluorescence-NBP3-15671-img0002.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of various cell lines, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0003.jpg)
![Immunoprecipitation: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Smad2 antibody (NBP3-15671). Western blot was performed from the immunoprecipitate using Smad2 antibody (NBP3-15671) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunoprecipitation-NBP3-15671-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Smad2 Antibody (2E3D8) [Smad2] - Western blot analysis of lysates from wild type (WT) and Smad2 knockdown (KD) HeLa cells using [KD Validated] Smad2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-52202517304995.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad2 Antibody (2E3D8) [Smad2] - Confocal imaging of HeLa cells using [KD Validated] Smad2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-522025173455.jpg)
![Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extracts from Hela cells untreated or treeated with PMAA using Smadd2 (Phospho-Thhr220) Antibody .](http://images.novusbio.com/fullsize/Smad2-[p-Thr220]-Antibody-Western-Blot-NBP1-04982-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extracts from Hela cells untreated or treeated with PMAA using Smadd2 (Phospho-Thhr220) Antibody .")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Smad2 [p Thr220] Antibody [NBP1-04982] - Immunohistochemical analysis of Smad2 staining in human brain formalin fixed paraffin embedded tissue section.](http://images.novusbio.com/fullsize/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-234202415232511.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad2 [p Thr220] Antibody [NBP1-04982] - Immunofluorescent analysis of Smad2 staining in HeLa cells.](http://images.novusbio.com/fullsize/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-234202414462213.jpg)
![Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Western blot analysis of Smad2 [p Thr220] expression in rat heart (A) whole cell lysates.](http://images.novusbio.com/fullsize/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-23420241452107.jpg)