| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81445-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2521SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-207SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87249-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49476-25ul | |||
![Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - Fetuin-A inhibits ciliogenesis. Primary cilia were examined in the absence (CTL) or presence of fetuin-A (FA) by immunostaining with antibodies against acetylated tubulin (Ace-tub), Cep164, IFT88, or Arl13b. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/20/20/5207), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81445-img0014.jpg "Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - Fetuin-A inhibits ciliogenesis. Primary cilia were examined in the absence (CTL) or presence of fetuin-A (FA) by immunostaining with antibodies against acetylated tubulin (Ace-tub), Cep164, IFT88, or Arl13b. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/20/20/5207), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - Staining of human cell line U-2 OS shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81445-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - CEP295 overexpression induces extra-long microtubule-based filaments with low efficiency. Schematics on right of D indicate centrioles (black rectangles) and positions of CEP164 (red squares). MC, mother centriole; DC, daughter centriole. Image collected and cropped by CiteAb from the following publication (//jcs.biologists.org/lookup/doi/10.1242/jcs.186338), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81445-img0013.jpg)
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0008.jpg)
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human bronchus shows moderate to strong positivity in cilia in respiratory epithelial cells.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0009.jpg)
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human nasopharynx shows strong positivity in cilia in respiratory epithelial cells.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0010.jpg)
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human endometrium shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Super-resolution (3D-SIM) microscopic analysis of centriolar distal-end proteins. (a) RPE1 cells were synchronized at G2 phase & immunostained with the indicated antibodies. Lower panels show fluorescence profile plots. Representative images of more than ten cells are presented in (a). ND: nascent daughter centriole, M: mother centriole. (b) The diameters of C2CD3, Talpid3, CEP120, & CEP164 protein spots (n = 30/each). Cumulative data from three independent experiments are shown. ***P < 0.001. (c,d) 3D-SIM analysis of the spatial localizations of C2CD3 (c), Talpid3 (d) & CEP120 (c,d) at the distal ends of centrioles. RPE1 cells were synchronized at G2 phase & immunostained with the indicated antibodies. Scale bar, 100 nm in (a,c,d). (e) Schematic illustration of the spatial localizations of C2CD3, Talpid3, CEP120, & CP110 at the distal ends of centrioles. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30988386), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81445_rabbit-polyclonal-cep164-antibody-310202416113546.jpg)
![Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Depletion of RTTN produces shorter centrioles. a, b U2OS cells were treated with siControl or siRTTN as shown in a, & analyzed by confocal fluorescence microscopy using the indicated antibodies. cHistogram illustrating the distance between the CEP162-positive dots associated with a given pair of orthogonally oriented centrioles. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). d, f PLK4-myc doxycycline (Dox) inducible cells were treated with siControl or siRTTN as shown in a, & then analyzed by confocal fluorescence microscopy using the indicated antibodies d, or by electron microscopy f. e, gHistogram illustrating the length of procentrioles in PLK4-myc-inducible cells, as analyzed by confocal microscopy e or electron microscopy g. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). The procentriole length in b/c was measured as the distance between two CEP162 dots as described by Azimzadeh et al.17 The procentriole length in d/e was measured as the distance between the fluorescent peak intensity of SAS-6 (red) & CEP162 (green) in siControl & siRTTN-treated cells. Scale bar, 5 μm in b & d; Scale bar, 200 nm in f Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00305-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81445_rabbit-polyclonal-cep164-antibody-310202416121961.jpg)
![Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - CPAP-E1235V mutation impaired the recruitment of centriole elongation proteins onto the centrioles. Cells were synchronized in the early S phase by treatment with aphidicolin (2 μg/ml) for 24 h. They were then fixed immediately with methanol (S phase centrioles) or released into fresh culture medium without aphidicolin for another 16 h (G2 phase centrioles). CPAP-WT or mutant cells in the S phase or G2 phase were immunostained with antibodies against the following centriolar proteins. (A) STIL, n = 42 for CPAP-WT; n = 25 for CPAP-E1235V#1; n = 34 for CPAP-E1235V#2. (B) CEP120, n = 50 for CPAP-WT; n = 42 for CPAP-E1235V#1; n = 48 for CPAP-E1235V#2. (C) CENTROBIN, n = 40 for all groups. (D) CEP295, n = 40 for all groups. (E) POC5, n = 42 for CPAP-WT; n = 40 for CPAP-E1235V#1; n = 40 for CPAP-E1235V#2. (F) POC1B, n = 40 for all groups. (G) CEP164, n = 88 for CPAP-WT; n = 74 for CPAP-E1235V#1; n = 38 for CPAP-E1235V#2. All data are presented as mean ±SEM from a pool of n cells from three independent experiments. n.s: not significant; **p < 0.01; ***p < 0.001. Scale bar: 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35309908), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81445_rabbit-polyclonal-cep164-antibody-31020241613623.jpg)
![Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Depletion of RTTN produces shorter centrioles. a, b U2OS cells were treated with siControl or siRTTN as shown in a, & analyzed by confocal fluorescence microscopy using the indicated antibodies. cHistogram illustrating the distance between the CEP162-positive dots associated with a given pair of orthogonally oriented centrioles. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). d, f PLK4-myc doxycycline (Dox) inducible cells were treated with siControl or siRTTN as shown in a, & then analyzed by confocal fluorescence microscopy using the indicated antibodies d, or by electron microscopy f. e, gHistogram illustrating the length of procentrioles in PLK4-myc-inducible cells, as analyzed by confocal microscopy e or electron microscopy g. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). The procentriole length in b/c was measured as the distance between two CEP162 dots as described by Azimzadeh et al.17 The procentriole length in d/e was measured as the distance between the fluorescent peak intensity of SAS-6 (red) & CEP162 (green) in siControl & siRTTN-treated cells. Scale bar, 5 μm in b & d; Scale bar, 200 nm in f Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00305-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81445_rabbit-polyclonal-cep164-antibody-310202416114711.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Spy1 protein levels are elevated in human breast cancers. TMAs containing cores from invasive ductal carcinoma (IvDC), infiltrated ductal carcinoma (IfDC), intraductal carcinoma (IDC) and invasive lobular carcinoma (ILC) as well as pair-matched normal (PM-N) or cancer-free patients (CF-N) were analyzed for Spy1 expression. The Spy1 signal intensity was normalized to nuclear stain (TOTO-3/PI) signal. Patient numbers are indicated below the sample (N). Breast normal and cancer cell lines were analyzed by western blot analysis. Actin was used as a loading control. One representative blot of 2. Image collected and cropped by Citeab from the following publication (The cyclin-like protein Spy1/RINGO promotes mammary transformation and is elevated in human breast cancer. BMC Cancer (2012)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Western-Blot-NB100-2521-img0006.jpg "Western Blot: Speedy/Ringo Antibody [NB100-2521] - Spy1 protein levels are elevated in human breast cancers. TMAs containing cores from invasive ductal carcinoma (IvDC), infiltrated ductal carcinoma (IfDC), intraductal carcinoma (IDC) and invasive lobular carcinoma (ILC) as well as pair-matched normal (PM-N) or cancer-free patients (CF-N) were analyzed for Spy1 expression. The Spy1 signal intensity was normalized to nuclear stain (TOTO-3/PI) signal. Patient numbers are indicated below the sample (N). Breast normal and cancer cell lines were analyzed by western blot analysis. Actin was used as a loading control. One representative blot of 2. Image collected and cropped by Citeab from the following publication (The cyclin-like protein Spy1/RINGO promotes mammary transformation and is elevated in human breast cancer. BMC Cancer (2012)) licensed under a CC-BY license.")
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Spy1 stable protein induces anchorage independent growth. Representative foci after 14 day soft agar assay visualized using light microscopy. Ras-V12 is transfected as a positive control. n = 3. Total numbers of foci were counted over 3 separate plates using separate transfections for each experiment. n = 3. Error bars reflect SE between triplicate experiments. t test was performed. Western blot analysis of experiments performed. Quantified using densitometry followed by normalization for Actin levels. Image collected and cropped by Citeab from the following publication (The cyclin-like protein Spy1/RINGO promotes mammary transformation and is elevated in human breast cancer. BMC Cancer (2012)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Western-Blot-NB100-2521-img0005.jpg)
![Immunohistochemistry-Paraffin: Speedy/Ringo Antibody [NB100-2521] - Speedy/Ringo was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human Speedy/Ringo polyclonal Antibody (Catalog # NB100-2521) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of sperm cells.](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Immunohistochemistry-Paraffin-NB100-2521-img0004.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Detection of Speedy/Ringo in mouse testis (2ug/ml).](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Western-Blot-NB100-2521-img0003.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Elevated Spy1 levels prevent contact inhibition. (A) Representative views of focus formation assays in NIH3T3 cells transfected with Myc-Spy1-WT, Myc-Spy1-TST, positive control Ras-V12 or empty vector PCS3 control. n = 3 (B) Quantification of the number of colonies. Error bars reflect SE between triplicate experiments. t test was performed;** P ≤ 0.01. n = 3 (right panel) Western blot analysis of one representative lysate from A-B. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-45), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-31020241534341.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Spy1 stable protein induces anchorage independent growth. (A) 293 cells were transfected with different concentrations of Myc-Spy1-WT, Myc-Spy1-TST or 30 μg of empty PCS3 vector control. Soft agar assay was carried out & plates photographed & quantified on day 14. Foci were averaged over 3 separate transfections for each experiment. n = 3 (B) Western blot analysis of one representative experiment; densitometry in lower panel. n = 3 (C) Representative foci after 14 day soft agar assay visualized using light microscopy. Ras-V12 is transfected as a positive control. n = 3. (D) Total numbers of foci were counted over 3 separate plates using separate transfections for each experiment. n = 3. Error bars reflect SE between triplicate experiments. t test was performed;** P ≤ 0.01. (E) Western blot analysis of experiments in Figure. 1C & D. Quantified using densitometry followed by normalization for Actin levels. (A-E) Error bars reflect SE between triplicate experiments. t test was performed;* P≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-45), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-310202415541987.jpg)
![Immunocytochemistry/ Immunofluorescence: Speedy/Ringo Antibody [NB100-2521] - CDC2 associates with SPDY at ERES. (A-C) 0 h GV stage oocyte labeled for CDC2 (A, green), DNA (A, blue), & SPDY (B, red). Both CDC2 & SPDY localize to the same cortical domain (C). Note that the center of this oocyte is dented (the area that contains the arrow) causing the structure to appear in the middle of the oocyte, whereas it is located in the cortex. (D-F) 0 h GV stage oocyte stained for Cyclin B (D, green), DNA (D, blue), & P-GM130 (E, red). Cyclin B did not localize to the P-GM130-labeled cortical domain (F). (G-I) 0 h GV stage oocyte labeled for PSTAIR (G, green), DNA (G, blue), & P-GM130 (H, red). Spatial overlap of PSTAIR & phosphorylated GM130 staining (yellow) in a cortical domain is evident in the merged image (I). Images are Z-projections of 2 (A-C) or 3 (D-I) consecutive sections; scale bars represent 20 μm in C, F, & I, & 5 μm in C', F', & I'. Arrows indicate the region of the oocyte that is shown enlarged in the insets (A'-I'). Arrowheads denote the position of the GV. Image collected & cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-9-8), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-31020241555219.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Spy1 stable protein accelerates tumorigenesis in vivo.(A) Percentage of mice presenting with palpable tumors from 0-19 days post-transplant. Each data point represents 4 mice per indicated construct. The entire experiment was repeated three times using three independently derived overexpressing cell lines for each construct. Mann-Whitney Test was performed (p < 0.05). (A; lower blot) Western blots were conducted to measure the stability of Myc-Spy1-WT (left) & Myc-Spy1-TST (right) from representative time points. Empty vector control (Cntl) cell expression levels of Spy1 are seen in lane 1 of each blot. (B) Total tumor volume was calculated for both Spy1-HC11 (Spy1-WT) & Spy1-TST-HC11 (Spy1-TST) transplanted glands. Results were taken from 45 transplants using cells from 3 separate transfections. Error bars reflect SE between transplants from different transfections. Left hand panel reflects overall volume, right hand panel reflects volumes of tissues taken over a month post-transplant. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-45), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-31020241553735.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Spy1 protein levels are elevated in human breast cancers.(A) TMAs containing cores from invasive ductal carcinoma (IvDC), infiltrated ductal carcinoma (IfDC), intraductal carcinoma (IDC) & invasive lobular carcinoma (ILC) as well as pair-matched normal (PM-N) or cancer-free patients (CF-N) were analyzed for Spy1 expression. The Spy1 signal intensity was normalized to nuclear stain (TOTO-3/PI) signal. Patient numbers are indicated below the sample (N). (B) Breast normal & cancer cell lines were analyzed by western blot analysis. Actin was used as a loading control. One representative blot of 2. (C) Western blot of 3 replicate infections of pLKO control or pLKO Spy1 in MDA-231 cells. Middle panel represents the densitometry values over 3 separate experiments. Where Spy1 is corrected for actin levels. Lower panel reflects cell counts at 4, 6, & 24 h post-infection. (All panels) Data shown is mean ± s.d. Student's t-test was performed * P < 0.05;**P < 0.01. (D) Knockdown effects on cell counts of MCF7 cells over 72 h after transfection with either pSUPER empty vector (siCntl) or pSUPER-Spy1 (siSpy1) over 3 separate transfections. Data shown is mean ± s.d. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-45), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-31020241555227.jpg)
![Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-SS-1-1-13-Western-Blot-NB500-207-img0001.jpg "Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.")
![Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).](http://images.novusbio.com/fullsize/CHAF1A-Antibody-SS-1-1-13-Immunocytochemistry-Immunofluorescence-NB500-207-img0004.jpg)
![Western Blot: CHAF1A Antibody [NB100-74608] - RECQ5 promotes SUMO2 conjugation of PCNA. Western blot analysis of the indicated proteins in WCE prepared from control or RECQ5 siRNA knockdown HEK293T cells with exogenous overexpression of indicated FLAG-tagged PCNA constructs or CAF1 (CHAF1A) or treated with an empty vector (V). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05236-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74608-img0012.jpg "Western Blot: CHAF1A Antibody [NB100-74608] - RECQ5 promotes SUMO2 conjugation of PCNA. Western blot analysis of the indicated proteins in WCE prepared from control or RECQ5 siRNA knockdown HEK293T cells with exogenous overexpression of indicated FLAG-tagged PCNA constructs or CAF1 (CHAF1A) or treated with an empty vector (V). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05236-y), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74608] - Human colon carcinoma peyer's patch. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Immunohistochemistry-Paraffin-NB100-74608-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CHAF1A Antibody [NB100-74608] - Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74608. Caf1p150 was also immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74609 and NB100-74610.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74608-img0011.jpg)
![Western Blot: CHAF1A Antibody [NB100-74608] - SUMO2-PCNA enriches CAF1 & FACT within the replication complex. a Western blot analysis of the indicated proteins in the CB fractions (left) & the FLAG-PCNA complexes purified from CB fractions (right) prepared from cells expressing the indicated FLAG-tagged proteins. Ub-KR indicates the FLAG-ubiquitin-PCNA (KR) fusion protein. b Western blot analysis of the indicated proteins in the CB input (left panels) used for the in vitro pull-down experiments of purified recombinant strep-tagged PCNA, SUMO2-conjugated PCNA, & GFP anchored to Strep-Tactin resins (right panels). SUMO2-modified PCNA was generated by the in vitro SUMOylation reaction using strep-PCNA & His-SUMO2 shown in Supplementary Fig. 6a, b. c Western blot analysis of the input levels of recombinant strep-PCNA & strep-SUMO2-PCNA fusion (S2-PCNA) purified from E. coli (left panel) & of strep-PCNA & strep-S2-PCNA pulled down by WT or SIM mutant FLAG-CAF1A proteins purified & anchored on FLAG M2 agarose beads from HEK293T cells expressing the corresponding constructs (right panels). d Western blot analysis of strep-S2-PCNA (upper 2 panels) & strep-PCNA (lower 2 panels) pulled down by FLAG-CAF1A purified from E. coli & anchored on FLAG M2 agarose beads. e Western blot analysis of the indicated proteins in the CB input prepared from HEK293T cells expressing FLAG-SSRP1 WT or SIM mutant (left panels) & of FLAG-SSRP1 & SPT16 pulled down from the CB fractions in vitro using purified recombinant strep-tagged PCNA, S2-PCNA, & GFP anchored to Strep-Tactin resins (right panels) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30006506), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74608_rabbit-polyclonal-chaf1a-antibody-310202416575.jpg)
![Western Blot: CHAF1A Antibody [NB100-74609] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caf1p150 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74609-img0003.jpg "Western Blot: CHAF1A Antibody [NB100-74609] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caf1p150 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74609] - Human lung cancer. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Immunohistochemistry-Paraffin-NB100-74609-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CHAF1A Antibody [NB100-74609] - Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74609. Caf1p150 was also immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74608 and NB100-74610.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74609-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHAF1A Antibody [NB100-74609] - Detection of human Caf1p150 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Caf1p150 antibody NB100-74609 used for IP at 3 ug per reaction. Caf1p150 was also immunoprecipitated by another rabbit anti-Caf1p150 antibody. For blotting immunoprecipitated Caf1p150, NB100-74609 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Immunoprecipitation-NB100-74609-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP1-87249] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87249-img0007.jpg "Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP1-87249] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0011.jpg)
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human liver shows moderate to strong nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0008.jpg)
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0009.jpg)
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in white matter.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0010.jpg)
![Western Blot: PPM1D Antibody [NBP3-12941] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PPM1D antibody (NBP3-12941) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Western-Blot-NBP3-12941-img0005.jpg "Western Blot: PPM1D Antibody [NBP3-12941] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PPM1D antibody (NBP3-12941) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP3-12941] - PPM1D antibody detects PPM1D protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPM1D stained by PPM1D antibody (NBP3-12941) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12941-img0004.jpg)
![Western Blot: PPM1D Antibody [NBP3-12941] - Jurkat whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPM1D antibody (NBP3-12941) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Western-Blot-NBP3-12941-img0001.jpg)
![Western Blot: PPM1D Antibody [NBP3-12941] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPM1D antibody (NBP3-12941) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Western-Blot-NBP3-12941-img0002.jpg)
![Western Blot: PPM1D Antibody [NBP3-12941] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPM1D antibody (NBP3-12941) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Western-Blot-NBP3-12941-img0003.jpg)
![Western Blot: PPM1D Antibody [NBP2-81881] - Analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Western-Blot-NBP2-81881-img0001.jpg "Western Blot: PPM1D Antibody [NBP2-81881] - Analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: PPM1D Antibody - BSA Free [NBP2-81881] - Immunofluorescence of PPM1D in human lung carcinoma tissue with PPM1D antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-81881_rabbit-polyclonal-ppm1d-antibody-271120242074637.jpg)
![Immunohistochemistry: PPM1D Antibody - BSA Free [NBP2-81881] - Immunohistochemistry of PPM1D in human lung carcinoma tissue with PPM1D antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81881_rabbit-polyclonal-ppm1d-antibody-2711202419531610.jpg)
![Immunohistochemistry: CEP164 Antibody - BSA Free [NBP3-47644] - Immunohistochemistry of paraffin-embedded mouse brain using ( CEP164 Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47644_rabbit-cep164-pab-bsa-free-284202511414846.jpg "Immunohistochemistry: CEP164 Antibody - BSA Free [NBP3-47644] - Immunohistochemistry of paraffin-embedded mouse brain using ( CEP164 Antibody) at dilution of 1:50")
![Western Blot: CEP164 Antibody - BSA Free [NBP3-47644] - HEK-293 cells were subjected to SDS PAGE followed by western blot with ( CEP164 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-47644_rabbit-cep164-pab-bsa-free-284202511425511.jpg)
![Immunohistochemistry-Paraffin: Speedy/Ringo Antibody [NBP2-49476] - Staining in human testis and fallopian tube tissues using anti-SPDYA antibody. Corresponding SPDYA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Immunohistochemistry-Paraffin-NBP2-49476-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Speedy/Ringo Antibody [NBP2-49476] - Staining in human testis and fallopian tube tissues using anti-SPDYA antibody. Corresponding SPDYA RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Speedy/Ringo Antibody [NBP2-49476] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Immunohistochemistry-Paraffin-NBP2-49476-img0003.jpg)
![Immunohistochemistry-Paraffin: Speedy/Ringo Antibody [NBP2-49476] - Staining of human fallopian tube shows low expression as expected.](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Immunohistochemistry-Paraffin-NBP2-49476-img0002.jpg)
![Western Blot: CEP164 Antibody (17) [NBP2-43707] - Analysis of whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Cep 164 antibody diluted by 1:10000.](http://images.novusbio.com/fullsize/CEP164-Antibody-17-Western-Blot-NBP2-43707-img0002.jpg "Western Blot: CEP164 Antibody (17) [NBP2-43707] - Analysis of whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Cep 164 antibody diluted by 1:10000.")
![Western Blot: CEP164 Antibody (17) [NBP2-43707] - Analysis of 30 ug whole cell lysate. A: HeLa 5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/CEP164-Antibody-17-Western-Blot-NBP2-43707-img0001.jpg)