| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86805-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87488-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87925-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87926-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88648-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27073SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39055-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61844-0.025ml | |||
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex, gallbladder, kidney and lymph node using Anti-EML4 antibody NBP1-86805 (A) shows similar protein distribution across tissues to independent antibody NBP2-38319 (B).](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0007.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human lymph node.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0010.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0008.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human kidney.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0009.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0011.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86805_rabbit-polyclonal-eml4-antibody-304202513101431.jpg)
![Immunocytochemistry/Immunofluorescence: Aldolase A Antibody [NBP1-87488] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87488-img0008.jpg "Immunocytochemistry/Immunofluorescence: Aldolase A Antibody [NBP1-87488] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![Aldolase A Antibody [NBP1-87488] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-immunohistochemistry-paraffin-1852023111210..jpg)
![Aldolase A Antibody [NBP1-87488] -Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-immunohistochemistry-paraffin-1852023111334..jpg)
![Aldolase A Antibody [NBP1-87488] -Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-immunohistochemistry-paraffin-1852023111457..jpg)
![Aldolase A Antibody [NBP1-87488] -Staining of human testis shows weak cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-immunohistochemistry-paraffin-1852023111636..jpg)
![Aldolase A Antibody [NBP1-87488] -Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-western-blot-1852023111745..jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0016.jpg "Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of monoclonal antibody to NME2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Immunocytochemistry-Immunofluorescence-H00004831-M06-img0009.jpg)
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of monoclonal antibody to NME2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Immunohistochemistry-Paraffin-H00004831-M06-img0011.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0013.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0014.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0015.jpg)
![Sandwich ELISA: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Detection limit for recombinant GST tagged NME2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Sandwich-ELISA-H00004831-M06-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87925] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87925-img0007.jpg "Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87925] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.")
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0011.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human cervix, uterine shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0008.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0009.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0010.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/nbp1-87925_rabbit-polyclonal-flightless-i-antibody-65202516291312.jpg)
![Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87926] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87926-img0010.jpg "Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87926] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.")
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0014.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0011.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human gastrointestinal shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0012.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0013.jpg)
![Western Blot: Flightless I Antibody [NBP1-87926] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP1-87926-img0006.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining in human cerebral cortex and prostate tissues using NBP1-88648 antibody. Corresponding FABP7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining in human cerebral cortex and prostate tissues using NBP1-88648 antibody. Corresponding FABP7 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: FABP7/B-FABP Antibody [NBP1-88648] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP1-88648-img0013.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human cerebellum shows strong cytoplasmic positivity in Bergmann glia.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0014.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0015.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human glioma shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0016.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0017.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0018.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0010.jpg "Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.")
![Western Blot: Aldolase A Antibody [NB600-915] - Figure. Immunoblotting. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 degrees C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0003.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0009.jpg)
![Immunoprecipitation: Aldolase A Antibody [NB600-915] - Analysis using the Biotin conjugate of NB600-915. Detection of 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 ug/ml) of purified aldolase in PBS. Precipitation of aldolase was confirmed.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Immunoprecipitation-NB600-915-img0005.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Twenty one more markedly oxidized proteins identified in 6‐week‐old PLP‐tg66/66 mice in the spinal cord. All non‐mitochondrial oxidized proteins tested by Western blot in the spinal cord are normally expressed. Relative protein level expressed as a percentage of control and in reference to gamma ‐tubulin as a loading marker (n = 6/genotype). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29027761/) licensed under a CC-BY license](http://images.novusbio.com/fullsize/nb600-915_goat-polyclonal-aldolase-a-antibody-2552023153959.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in human spleen in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, and 4) mouse embryonic brain lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP2-27073-img0004.jpg "Western Blot: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in human spleen in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, and 4) mouse embryonic brain lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-27073-img0003.jpg)
![Western Blot: Vasohibin Antibody (4A3) [H00022846-M05] - Analysis of VASH1 expression in HepG2 (Cat # L019V1).](http://images.novusbio.com/fullsize/Vasohibin-Antibody-4A3-Western-Blot-H00022846-M05-img0004.jpg "Western Blot: Vasohibin Antibody (4A3) [H00022846-M05] - Analysis of VASH1 expression in HepG2 (Cat # L019V1).")
![Immunohistochemistry-Paraffin: Vasohibin Antibody (4A3) [H00022846-M05] - Analysis of monoclonal antibody to VASH1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Vasohibin-Antibody-4A3-Immunohistochemistry-Paraffin-H00022846-M05-img0002.jpg)
![Sandwich ELISA: Vasohibin Antibody (4A3) [H00022846-M05] - Detection limit for recombinant GST tagged VASH1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Vasohibin-Antibody-4A3-Sandwich-ELISA-H00022846-M05-img0003.jpg)
![Western Blot: EML4 Antibody [NBP2-39055] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HepG2](http://images.novusbio.com/fullsize/EML4-Antibody-Western-Blot-NBP2-39055-img0002.jpg "Western Blot: EML4 Antibody [NBP2-39055] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HepG2")
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0010.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0004.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining in human appendix and cerebral cortex tissues using anti-EML4 antibody. Corresponding EML4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human cerebral cortex, gallbladder, kidney and lymph node using Anti-EML4 antibody NBP2-39055 (A) shows similar protein distribution across tissues to independent antibody NBP2-38319 (B).](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0007.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human kidney.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0008.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human lymph node.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0009.jpg)
![Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against HEK293 (1) and ALDOA (AA: 9-145)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Western-Blot-NBP2-61844-img0006.jpg "Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against HEK293 (1) and ALDOA (AA: 9-145)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of breast cancer tissues using ALDOA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Immunohistochemistry-NBP2-61844-img0004.jpg)
![Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against human ALDOA (AA: 9-145) recombinant protein. (Expected MW is 40.7 kDa)](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Western-Blot-NBP2-61844-img0005.jpg)
![Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mouse mAb against MCF-7 (1), Hela (2), and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Western-Blot-NBP2-61844-img0007.jpg)
![Immunohistochemistry-Paraffin: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of bladder cancer tissues using ALDOA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Immunohistochemistry-NBP2-61844-img0003.jpg)
![ELISA: Aldolase A Antibody (1C5B2) [NBP2-61844] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-ELISA-NBP2-61844-img0001.jpg)
![ELISA: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of K562 cells using ALDOA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-ELISA-NBP2-61844-img0002.jpg)
![Immunoprecipitation: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Peroxiredoxin 5 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32740 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32740 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32740 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32740 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-26820241582610.jpg "Immunoprecipitation: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Peroxiredoxin 5 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32740 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32740 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32740 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32740 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL")
![Immunohistochemistry: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32740) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-298202414262615.jpg)
![Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 5 with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-292024111484.jpg)
![Flow Cytometry: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Flow cytometric analysis of HeLa cells labeling Peroxiredoxin 5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32740, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-29202416372132.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Western blot analysis of Peroxiredoxin 5 on different lysates with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/50,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: MCF7 cell lysate (10 µg/Lane) Lane 4: Mouse liver tissue lysate (15 µg/Lane) Lane 5: Mouse kidney tissue lysate (15 µg/Lane) Lane 6: Rat liver tissue lysate (15 µg/Lane) Lane 7: Rat kidney tissue lysate (15 µg/Lane) Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-3920248453533.jpg)