| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24394SS | |||
| Azide and BSA Free | NBP2-33254 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56564SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33066 | |||
| IHC-Prediluted | NBP2-44365 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56582SS | |||
| Azide and BSA Free | NBP2-33297 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-28632SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88071-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70078 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71667 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94636-0.02ml | |||
![Western Blot: LC3A Antibody [NBP2-24394] - Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with ATG8 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP2-24394-img0007.jpg "Western Blot: LC3A Antibody [NBP2-24394] - Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with ATG8 antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-24394] - Staining of Human brain probed with ATG8 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-24394-img0006.jpg)
![Western Blot: LC3A Antibody [NBP2-24394] - The expression of LC3A in rat tissue. Antibody dilution 1:1000. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP2-24394-img0005.jpg)
![Western Blot: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # NB100-56564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Knockout-Validated-NB100-56564-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # NB100-56564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-1 Antibody [NB100-56564] - Analysis of Caspase-1 using a polyclonal antibody. Human HeLa lysate probed with Caspase-1 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Western-Blot-NB100-56564-img0005.jpg)
![Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564] - Human reactive lymph node stained for Caspase-1 expression (NB100-56564) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Immunohistochemistry-Paraffin-NB100-56564-img0004.jpg)
![Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Western-Blot-NBP2-29439-img0011.jpg "Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0010.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Mouse Monoclonal Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0012.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin paraffin breast cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0003.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-paraffin ovarian cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0004.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0008.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0009.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0007.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33177-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33177] - Methanol-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33177-img0008.jpg)
![Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33177] - H1299 xenograft, using Gelsolin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Immunohistochemistry-Paraffin-NBP1-33177-img0009.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33177-img0011.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33177-img0012.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33177-img0014.jpg)
![Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (NBP1-33177) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp1-33177_rabbit-polyclonal-gelsolin-gsn-antibody-western-blot-2611202518233017.jpg)
![Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (NBP1-33177) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33177_rabbit-polyclonal-gelsolin-gsn-antibody-western-blot-2611202518315411.jpg)
![Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/mL.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Western-Blot-NB100-56582-img0018.jpg "Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/mL.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody. 1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0010.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody, 5 ug/10^6 cells. Products used: anti-CD4 PE conjugate, anti-CD25 APC conjugate, anti-FOXP3 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0013.jpg)
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of 2 x 10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0012.jpg)
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti-CD25 APC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0017.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.1 ug/10^6 cells. Anti-CD25 FITC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0014.jpg)
![Western Blot: EDIL3/DEL1 Antibody [NBP1-28632] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.](http://images.novusbio.com/fullsize/EDIL3-DEL1-Antibody-Western-Blot-NBP1-28632-img0005.jpg "Western Blot: EDIL3/DEL1 Antibody [NBP1-28632] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.")
![Immunocytochemistry/Immunofluorescence: EDIL3/DEL1 Antibody [NBP1-28632] - HeLa cells stained NBP1-28632 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).](http://images.novusbio.com/fullsize/EDIL3-DEL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28632-img0004.jpg)
![Simple Western: EDIL3/DEL1 Antibody [NBP1-28632] - Simple Western lane view shows a specific band for EDIL3/DEL1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EDIL3-DEL1-Antibody-Simple-Western-NBP1-28632-img0006.jpg)
![Western Blot: RALBP1 Antibody (2A1) [H00010928-M02] - RALBP1 monoclonal antibody (M02), clone 2A1 Analysis of RALBP1 expression in HeLa.](http://images.novusbio.com/fullsize/RALBP1-Antibody-2A1-Western-Blot-H00010928-M02-img0006.jpg "Western Blot: RALBP1 Antibody (2A1) [H00010928-M02] - RALBP1 monoclonal antibody (M02), clone 2A1 Analysis of RALBP1 expression in HeLa.")
![Western Blot: RALBP1 Antibody (2A1) [H00010928-M02] - Analysis of RALBP1 transfected lysate using anti-RALBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RALBP1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-2A1-Western-Blot-H00010928-M02-img0004.jpg)
![Western Blot: RALBP1 Antibody (2A1) [H00010928-M02] - RALBP1 monoclonal antibody (M02), clone 2A1. Analysis of RALBP1 expression in C32.](http://images.novusbio.com/fullsize/RALBP1-Antibody-2A1-Western-Blot-H00010928-M02-img0007.jpg)
![ELISA: RALBP1 Antibody (2A1) [H00010928-M02] - Detection limit for recombinant GST tagged RALBP1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-2A1-ELISA-H00010928-M02-img0008.jpg)
![Western Blot: Radixin Antibody (1D9) [H00005962-M06] - Analysis of RDX expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Radixin-Antibody-1D9-Western-Blot-H00005962-M06-img0004.jpg "Western Blot: Radixin Antibody (1D9) [H00005962-M06] - Analysis of RDX expression in HeLa (Cat # L013V1).")
![Immunocytochemistry/Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06] - Analysis of monoclonal antibody to RDX on HeLa cell. Antibody concentration 30 ug/ml](http://images.novusbio.com/fullsize/Radixin-Antibody-1D9-Immunocytochemistry-Immunofluorescence-H00005962-M06-img0003.jpg)
![Immunohistochemistry: Radixin Antibody (1D9) [H00005962-M06] - Decreased phosphorylation of radixin in the RMS after DX52-1 treatment. Merged image of radixin and phosphorylated rzrin/radixin/moesin (pERM) immunoreactivity in a coronal section of the control RMS. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2013.00161/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Radixin-Antibody-1D9-Immunohistochemistry-H00005962-M06-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06] - Decreased phosphorylation of radixin in the RMS after DX52-1 treatment. (A) Merged image of radixin & phosphorylated rzrin/radixin/moesin (pERM) immunoreactivity in a coronal section of the control RMS. (B) pERM immunoreactivity in the control RMS. (C) Merged image of radixin & phosphorylated ezrin/radixin/moesin (pERM) immunoreactivity in a coronal section in the DX52-1 treated RMS. (D) pERMimmunoreactivity in the DX52-1 treated RMS. Radixin/pERM immunopositive circular structures in B & D are likely residual ependymal cells originating from the wall of the collapsed olfactory ventricle (Peretto et al., 1997). Ependymal cells are known to express high levels of ezrin which explains sustained pERM expression in this area in both control & DX52-1 treated RMS. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24065889), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005962-m06_mouse-monoclonal-radixin-antibody-1d9-31020241548510.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Analysis in human skeletal muscle and liver tissues. Corresponding TTN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Analysis in human skeletal muscle and liver tissues. Corresponding TTN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Titin Antibody [NBP1-88071] - SMYD2 Cys13 glutathionylation or oxidation reduces myofibril integrity. Monitoring the myofibril alignment in rat neonatal cardiomyocytes upon incubation of AMA (2 ug/mL) for 12 h: no expression of SMYD2 WT. Immunostainings were done by using antibodies to SMYD2, HA (green), or titin (alpha-titin-NT, red). About 30 cells were photographed and examined for myofibril alignment or directionality by FiberFit software. 34 Images represent the major myofibril structure in individual conditions. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-06786-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88071-img0015.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0014.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0011.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0012.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: Titin Antibody [NBP1-88071] - SMYD2 Cys13 glutathionylation or oxidation reduces myofibril integrity. a, b Monitoring the myofibril alignment in rat neonatal cardiomyocytes upon incubation of AMA (2 µg/mL) for 12 h: no expression (a) & ectopic expression of SMYD2 WT or C13S (b). Immunostainings were done by using antibodies to SMYD2, HA (green), or titin ( alpha -titin-NT, red). About 30 cells were photographed & examined for myofibril alignment or directionality by FiberFit software.34 Images represent the major myofibril structure in individual conditions. Scale bars, 10 µm. c Analysis of myofibril alignment in cardiomyocytes. Individual cell images were analyzed by the FiberFit software to determine the fiber dispersion parameter (k) values that represent the degree of fiber alignment. High k values represent the aligned networks, whereas low k values represent the disordered networks. The median values with 95% CI are shown, n = 3 independent experiments. Difference is significant by one-way ANOVA, followed by Tukey’s post-hoc test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30337525), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88071_rabbit-polyclonal-titin-antibody-310202416121981.jpg)
![Immunocytochemistry/ Immunofluorescence: Titin Antibody [NBP1-88071] - SMYD2 Cys13 glutathionylation induces dissociation of SMYD2 from N2A & Hsp90. a, b SMYD2 glutathionylation disrupts its interaction with Hsp90. Purified SMYD2-SH & SMYD2-SSG were incubated with GST-Hsp90 bound to glutathione beads, & eluted sample was analyzed (a). Hsp90 was co-immunoprecipitated with SMYD2 WT or C13S from HEK293 cells in response to AMA with glucose deprivation (b). c, d SMYD2 glutathionylation disrupts its interaction with N2A. Purified SMYD2-SH & SMYD2-SSG were incubated with GST-N2A bound to glutathione beads, & eluted sample was analyzed (c). FLAG-N2A was co-immunoprecipitated with SMYD2 WT or C13S in HEK293 cells in response to AMA with glucose deprivation (d). e SMYD2 subjected to glutathionylation decreases its binding with N2A. SMYD2 WT or C13S was pre-incubated with H2O2 in the absence or presence of glutathione for 15 min, then mixed with GST-N2A bound to glutathione beads for 1 h. Eluted samples were analyzed. f, g Colocalization of titin & SMYD2 decreases upon incubation of AMA in rat neonatal cardiomyocytes expressing SMYD2 WT versus C13S. Immunostainings of cardiomyocytes with antibodies to titin ( alpha -titin-NT, red), HA, or SMYD2 (green) are shown with enlarged areas for details (the red boxes) (f). Pearson’s correlation coefficients were calculated to determine colocalization of titin & SMYD2 (g). About 30 cells were analyzed in individual conditions. Images represent the major colocalization pattern in individual experiments. Scale bars, 10 µm. Data represent the mean ± SD, n = 3 independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30337525), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88071_rabbit-polyclonal-titin-antibody-310202416114738.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0020.jpg "Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).")
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0016.jpg)
![Western Blot: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Western-Blot-NBP1-48251-img0018.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0012.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0013.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0014.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, and ACTN1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ACTN1 monoclonal antibody (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Knockout-Validated-NBP1-48251-img0021.jpg)
![Simple Western:alpha-Actinin 1 Antibody (OTI7A4)[NBP1-48251]Simple Western� analysis of endogenous protein Cytokeratin 8 from HELA lysates (0.5 mg/mL) using Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp1-48251_mouse-alpha-actinin-1-mab-oti7a4-simple-western-13112025123690.jpg%253fstore=america&image-type=image)
![Western Blot: KIF2C Antibody (OTI1C5) [NBP2-01064] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KIF2C monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Western-Blot-NBP2-01064-img0010.jpg "Western Blot: KIF2C Antibody (OTI1C5) [NBP2-01064] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KIF2C monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KIF2C .](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunocytochemistry-Immunofluorescence-NBP2-01064-img0011.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Human tonsil using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0009.jpg)
![Flow Cytometry: KIF2C Antibody (OTI1C5) [NBP2-01064] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KIF2C antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Flow-Cytometry-NBP2-01064-img0001.jpg)
![Western Blot: KIF2C Antibody (OTI1C5) [NBP2-01064] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF2C.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Western-Blot-NBP2-01064-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0003.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0004.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0005.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0006.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0007.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Human Kidney tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0008.jpg)
![Western Blot: YTHDF3 Antibody [NBP2-94636] - Western blot analysis of extracts of HepG2 cells, using YTHDF3 antibody (NBP2-94636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/YTHDF3-Antibody-Western-Blot-NBP2-94636-img0004.jpg "Western Blot: YTHDF3 Antibody [NBP2-94636] - Western blot analysis of extracts of HepG2 cells, using YTHDF3 antibody (NBP2-94636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: YTHDF3 Antibody [NBP2-94636] - Immunohistochemistry of paraffin-embedded rat brain using YTHDF3 Rabbit pAb (NBP2-94636) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/YTHDF3-Antibody-Immunohistochemistry-Paraffin-NBP2-94636-img0003.jpg)
![Western Blot: YTHDF3 Antibody [NBP2-94636] - Western blot analysis of extracts of Mouse liver, using YTHDF3 antibody (NBP2-94636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/YTHDF3 Antibody-Western Blot-NBP2-94636-img0005.jpg)
![Immunohistochemistry-Paraffin: YTHDF3 Antibody [NBP2-94636] - Immunohistochemistry of paraffin-embedded mouse brain using YTHDF3 Rabbit pAb (NBP2-94636) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/YTHDF3-Antibody-Immunohistochemistry-Paraffin-NBP2-94636-img0002.jpg)
![Immunoprecipitation: YTHDF3 Antibody [NBP2-94636] - Immunoprecipitation analysis of 300ug extracts of HT-1080 cells using 3ug YTHDF3 antibody (NBP2-94636). Western blot was performed from the immunoprecipitate using YTHDF3 antibody (NBP2-94636) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/YTHDF3-Antibody-Immunoprecipitation-NBP2-94636-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: YTHDF3 Antibody - BSA Free [YTHDF3] - Immunofluorescence analysis of HeLa cells using YTHDF3 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94636_rabbit-polyclonal-ythdf3-antibody-5220251636335.jpg)
![Immunocytochemistry/ Immunofluorescence: YTHDF3 Antibody - BSA Free [YTHDF3] - Immunofluorescence analysis of NIH/3T3 cells using YTHDF3 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94636_rabbit-polyclonal-ythdf3-antibody-5220251638027.jpg)
