| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11267-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98910-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47695-0.1mg | |||
| IHC-Prediluted | NBP2-48243 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47669-0.1mg | |||
| IHC-Prediluted | NBP2-48196 | |||
![Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Western-Blot-NB120-11267-img0003.jpg "Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HM-2) [NB120-11267] - B35 cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Immunocytochemistry-Immunofluorescence-NB120-11267-img0002.jpg)
![Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [SA30-06] (NBP3-32248) at 1:5,000 dilution. Lane 1: Wild-type HepG2 whole cell lysate (20 ug). Lane 2/3: Cytokeratin 19 knockout HepG2 whole cell lysate (20 ug). NBP3-32248 was shown to specifically react with Cytokeratin 19 in wild-type HepG2 cells. No band was observed when Cytokeratin 19 knockout samples were tested. Wild-type and Cytokeratin 19 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Cytokeratin 19 Antibody (ET1601-6, 1/5,000) and anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-278202416285243.jpg "Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [SA30-06] (NBP3-32248) at 1:5,000 dilution. Lane 1: Wild-type HepG2 whole cell lysate (20 ug). Lane 2/3: Cytokeratin 19 knockout HepG2 whole cell lysate (20 ug). NBP3-32248 was shown to specifically react with Cytokeratin 19 in wild-type HepG2 cells. No band was observed when Cytokeratin 19 knockout samples were tested. Wild-type and Cytokeratin 19 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Cytokeratin 19 Antibody (ET1601-6, 1/5,000) and anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 19 antibody (NBP3-32248) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32248) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-298202414115431.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 (NBP3-32248). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 19 (NBP3-32248, red) at 1/500 dilution at +4℃ overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-298202414333689.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - ICC staining of Cytokeratin 19 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32248, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-29202410484831.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Flow cytometric analysis of Cytokeratin 19 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-32248, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-39202488466.jpg)
![Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-278202415212453.jpg "Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29820241435276.jpg)
![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 50μg/mL TNF-alpha for 20 minutes labeling RelA/NFkB p65 with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29202410484811.jpg)
![Flow Cytometry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Flow cytometric analysis of NIH/3T3 cells labeling RelA/NFkB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32648, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-2920241637214.jpg)
![Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910] - Immunochemical staining Cofilin 2 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-98910-img0005.jpg "Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910] - Immunochemical staining Cofilin 2 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910] - Immunochemical staining Cofilin 2 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-98910-img0004.jpg)
![Immunoprecipitation: Cofilin 2 Antibody [NBP2-98910] -Lane A:0.5 mg Raji Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 µL anti-CFL2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-CFL2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-98910_rabbit-polyclonal-cofilin-2-antibody-immunoprecipitation-852024115611..jpg)
![Western Blot: Cofilin 2 Antibody [NBP2-98910] -Lane A: Raji Whole Cell LysateLane B: Hela Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLysates/proteins at 30 μg per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/antibody/nbp2-98910_rabbit-polyclonal-cofilin-2-antibody-western-blot-852024115711..jpg)
![Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-98910] - Immunofluorescence staining of Cofilin 2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98910 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98910_rabbit-polyclonal-cofilin-2-antibody-23520251664721.png)
![Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-278202416285276.jpg "Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32207) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2982024142099.jpg)
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunocytochemistry analysis of HeLa cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2920241048483.jpg)
![Flow Cytometry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Flow cytometric analysis of HeLa cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32207, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-29202416372168.jpg)
![Western Blot: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/10,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: A549 cell lysate (10 ug/Lane) Lane 3: NIH/3T3 cell lysate (10 ug/Lane) Lane 4: L-929 cell lysate (10 ug/Lane) Lane 5: PC-12 cell lysate (10 ug/Lane) Lane 6: C6 cell lysate (10 ug/Lane) Lane 7: Vero cell lysate (10 ug/Lane) Lane 8: COS-1 cell lysate (10 ug/Lane) Lane 9: Mouse spleen tissue lysate (20 ug/Lane) Lane 10: Mouse stomach tissue lysate (20 ug/Lane) Lane 11: Rat kidney tissue lysate (20 ug/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32081) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32081_mouse-beta-tubulin-mab-a1-a4-r-2782024163163.jpg "Western Blot: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/10,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: A549 cell lysate (10 ug/Lane) Lane 3: NIH/3T3 cell lysate (10 ug/Lane) Lane 4: L-929 cell lysate (10 ug/Lane) Lane 5: PC-12 cell lysate (10 ug/Lane) Lane 6: C6 cell lysate (10 ug/Lane) Lane 7: Vero cell lysate (10 ug/Lane) Lane 8: COS-1 cell lysate (10 ug/Lane) Lane 9: Mouse spleen tissue lysate (20 ug/Lane) Lane 10: Mouse stomach tissue lysate (20 ug/Lane) Lane 11: Rat kidney tissue lysate (20 ug/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32081) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32081) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32081_mouse-beta-tubulin-mab-a1-a4-r-298202414333673.jpg)
![Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (NBP3-32081) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32081_mouse-beta-tubulin-mab-a1-a4-r-29202410484886.jpg)
![Flow Cytometry: beta Tubulin Antibody (A1-A4-R) [NBP3-32081] - Flow cytometric analysis of HeLa cells labeling beta Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32081, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32081_mouse-beta-tubulin-mab-a1-a4-r-29202416372154.jpg)
![Caspase-8 Activity Assay Kit (Colorimetric) [NBP2-54817] - The assay was performed on murine insulinoma cell line following Novu's protocol.](http://images.novusbio.com/fullsize/Caspase-8-Activity-Assay-Kit-Colorimetric-NBP2-54817-img0001.jpg "Caspase-8 Activity Assay Kit (Colorimetric) [NBP2-54817] - The assay was performed on murine insulinoma cell line following Novu's protocol.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0002.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).")
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (4C4.9 + S100B/1012)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunohistochemistry-Paraffin-NBP2-54559-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0001.jpg)
![Western Blot: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Azide-and-BSA-Free-Western-Blot-NBP2-54399-img0002.jpg "Western Blot: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).")
![Immunohistochemistry-Paraffin: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54399-img0001.jpg)
![Immunohistochemistry-Paraffin: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6) [NBP2-44448] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Heparan Sulfate Monoclonal Antibody (A7L6).](http://images.novusbio.com/fullsize/Endorepellin-Perlecan-Heparan-Sulfate-Proteoglycan-Antibody-A7L6-Immunohistochemistry-Paraffin-NBP2-44448-img0002.jpg "Immunohistochemistry-Paraffin: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6) [NBP2-44448] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Heparan Sulfate Monoclonal Antibody (A7L6).")
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRT/457) [NBP2-45309] - Rat Oviduct stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-KRT-457-Immunohistochemistry-Paraffin-NBP2-45309-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRT/457) [NBP2-45309] - Rat Oviduct stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).")
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRT/457) [NBP2-45309] - Human Bladder Carcinoma stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-KRT-457-Immunohistochemistry-Paraffin-NBP2-45309-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31326-img0009.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-31326] - Lung CA, using Cytokeratin 14 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-31326-img0004.jpg)
![Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31326_rabbit-polyclonal-cytokeratin-14-antibody-9102024195726.jpg)