| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43948 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43926 | |||
| BSA Free | NBP2-34478-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48575-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90361-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44195 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89346-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-75925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76658-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74052 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74904 | |||
![Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Western-Blot-NBP2-88937-img0008.jpg "Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of A-431 cells using the Anti-PIK3CB monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunocytochemistry-Immunofluorescence-NBP2-88937-img0001.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0007.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0002.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human colonshows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0003.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0004.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human stomach cancer shows moderate to strong nuclear positivity.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0005.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-beta-PIK3CB-Antibody-CL9518-Immunohistochemistry-Paraffin-NBP2-88937-img0006.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Western-Blot-NBP2-34478-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of MCF7 cells using the Anti-ESR1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunocytochemistry-Immunofluorescence-NBP2-34478-img0009.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human testis shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human endometrium shows strong nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0001.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human cervix shows strong nuclear positivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human fallopian tube shows strong immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human breast cancer shows moderate to strong nuclear positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0004.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48575 (A) shows similar protein distribution across tissues to independent antibody NBP2-48504 (B).](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0010.jpg)
![Western Blot: Nestin Antibody [NBP2-48575] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48575-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-48575] - Staining of human cell line AF22 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48575-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human kidney using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0013.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0006.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0011.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0012.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0014.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Analysis in human spleen and skeletal muscle tissues. Corresponding ASC/TMS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Analysis in human spleen and skeletal muscle tissues. Corresponding ASC/TMS1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human liver, lung, skeletal muscle and spleen using Anti-ASC/TMS1 antibody NBP2-48925 (A) shows similar protein distribution across tissues to independent antibody NBP2-49133 (B).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP2-48925] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus, nucleoli & cytosol.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48925-img0005.jpg)
![Western Blot: ASC/TMS1 Antibody [NBP2-48925] - Analysis in human spleen tissue.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP2-48925-img0017.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Immunohistochemical staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP2-48925-img0019.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0006.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human liver shows moderate cytoplasmic positivity in Kupffer cells.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0015.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0016.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP2-48925-img0018.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nbp1-90361_rabbit-polyclonal-adenosine-deaminase-ada-antibody-29520251613462.jpg)
![ELISA: Mouse VEGF-C ELISA Kit (Colorimetric) [NBP2-78893] - Samples were spiked with high concentrations of Mouse VEGF-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-78893_mouse-vegf-c-elisa-kit-colorimetric-132202416254038.jpg "ELISA: Mouse VEGF-C ELISA Kit (Colorimetric) [NBP2-78893] - Samples were spiked with high concentrations of Mouse VEGF-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse VEGF-C ELISA Kit (Colorimetric) [NBP2-78893] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-78893_mouse-vegf-c-elisa-kit-colorimetric-432024859725.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human skin and pancreas tissues using NBP2-88917 antibody. Corresponding GATA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human skin and pancreas tissues using NBP2-88917 antibody. Corresponding GATA3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human cell line TD47D.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Western-Blot-NBP2-88917-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of MCF7 cells using the Anti-GATA3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunocytochemistry-Immunofluorescence-NBP2-88917-img0001.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0002.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human kidney shows strong nuclear positivity in a small subset of cells in glomeruli.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0003.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0004.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0005.jpg)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0006.jpg)
![Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-LGALS3BP antibody. Corresponding LGALS3BP RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Western-Blot-NBP1-89346-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-LGALS3BP antibody. Corresponding LGALS3BP RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Immunohistochemistry-Paraffin-NBP1-89346-img0020.jpg)
![Immunohistochemistry-Paraffin: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Immunohistochemistry-Paraffin-NBP1-89346-img0017.jpg)
![Immunohistochemistry-Paraffin: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Immunohistochemistry-Paraffin-NBP1-89346-img0018.jpg)
![Immunohistochemistry-Paraffin: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Staining of human skeletal muscle shows no cytoplasmic positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Western-Blot-NBP1-89346-img0019.jpg)
![Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-75925-img0002.jpg "Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Analysis of a FFPE tissue section of the human intesting using 1:200 dilution of TNF-alpha antibody (NBP2-75925). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a moderate to strong cytoplasmic staining in crypt cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-75925-img0003.jpg)
![Neutralization: TNF-alpha Antibody (2342C) [NBP2-75925] - Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # NBP2-75925). The ND50 is typically 1-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Neutralization-NBP2-75925-img0001.jpg)
![Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Bmf Antibody [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bmf-Antibody-Western-Blot-NBP1-76658-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Bmf Antibody [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: Bmf Antibody [NBP1-76658] - Analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Bmf-Antibody-Western-Blot-NBP1-76658-img0005.jpg)
![Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs incubated w/ M-CSF (30 ng/ml) & RANKL (40 ng/ml) for 40 h, washed thoroughly, & incubated further w/ LPS (50 ng/ml) in presence of M-CSF (30 ng/ml) for 48 h. (a) Cells transfected w/ 30 nM of miR-29b mimic or con mimic in presence of M-CSF (30 ng/ml) for 6 h. Total RNA analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, & HRK. Expression levels w/ con mimic treatment set at 1. Cell lysates subjected to WB analysis w/ antibodies against BMF, PUMA, BAK1, BIM, HRK, & BCL-2. Antibodies against beta -actin used for normalization. (b) w/out transfection, total RNA analyzed by qPCR to quantify the expression of BMF, & cell lysates subjected to WB analysis w/ anti-BMF Ab. (c) Total RNA & tissue lysate of tibiae from LPS-treated or vehicle-treated (V, PBS) mice analyzed by qPCR to quantify the expression of BMF & subjected to WB analysis w/ antibodies against BMF. (d) Cells thoroughly washed, transfected w/ 30 nM of anti-miR-29b or con inh, & stimulated w/ LPS (50 ng/ml) in presence of M-CSF. After 48 h, total RNA analyzed by qPCR to quantify BMF expression. Expression levels w/ con inh treatment set at 1.0. Cell lysates subjected to WB analysis w/ anti-BMF & anti-BCL-2 Ab. (e) Cells thoroughly washed, transfected w/ 50 nM of scRNA or siBMF, stimulated w/ LPS (50 ng/ml) in presence of M-CSF for 48 h, & analyzed to measure TRAP-positive MNCs & annexin V-positive cells. siRNA-mediated silencing of BMF confirmed by RT-PCR & qPCR. The Ct values of genes widely distributed between 17.33 & 30.92. ∗p Red: Bmf Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76658_rabbit-polyclonal-bmf-antibody-2711202419432229.gif)
![Immunocytochemistry/ Immunofluorescence: Bmf Antibody - BSA Free [NBP1-76658] - Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76658_rabbit-polyclonal-bmf-antibody-271120242022971.jpg)
![Western Blot: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Secernin 1.](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Western-Blot-NBP2-45727-img0010.jpg "Western Blot: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Secernin 1.")
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0009.jpg)
![Western Blot: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Western-Blot-NBP2-45727-img0011.jpg)
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![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0005.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0006.jpg)
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![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0008.jpg)
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![Immunohistochemistry: XTP4 Antibody (OTI2D5) [NBP2-46592] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/XTP4-Antibody-OTI2D5-Immunohistochemistry-NBP2-46592-img0002.jpg)
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![Immunohistochemistry: XTP4 Antibody (OTI2D5) [NBP2-46592] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/XTP4-Antibody-OTI2D5-Immunohistochemistry-NBP2-46592-img0004.jpg)
![Immunohistochemistry: XTP4 Antibody (OTI2D5) [NBP2-46592] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/XTP4-Antibody-OTI2D5-Immunohistochemistry-NBP2-46592-img0005.jpg)
![Immunohistochemistry: XTP4 Antibody (OTI2D5) [NBP2-46592] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/XTP4-Antibody-OTI2D5-Immunohistochemistry-NBP2-46592-img0006.jpg)
![Immunohistochemistry: XTP4 Antibody (OTI2D5) [NBP2-46592] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/XTP4-Antibody-OTI2D5-Immunohistochemistry-NBP2-46592-img0007.jpg)
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![Immunohistochemistry: XTP4 Antibody (OTI2D5) [NBP2-46592] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/XTP4-Antibody-OTI2D5-Immunohistochemistry-NBP2-46592-img0009.jpg)
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