| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44939 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44493 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84450-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87778-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88057-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87455-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87779-25ul | |||
![Western Blot: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Western Blot Analysis of HCT116 cell lysate using CK8 with Cytokeratin 8 Antibody (K8.8).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Western-Blot-NBP2-34654-img0006.jpg "Western Blot: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Western Blot Analysis of HCT116 cell lysate using CK8 with Cytokeratin 8 Antibody (K8.8).")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8 Antibody (K8.8).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34654-img0005.jpg)
![Flow Cytometry: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 8 Antibody (K8.8) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34654-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Staining of human colon carcinoma.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34654-img0001.jpg)
![Simple Western: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 8 antibody was used at 1 ug/ml dilution of MCF-7 lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Simple-Western-NBP2-34654-img0003.jpg)
![Simple Western: Cytokeratin 8 Antibody (K8.8) - Azide and BSA Free [NBP2-34654] - Simple Western lane view shows a specific band for Cytokeratin 8 in 0.1 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-8-Azide-and-BSA-Free-Simple-Western-NBP2-34654-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34718-img0001.jpg "Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - p27/Kip1 antibody in CODEX stain of human bone marrow tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34718-img0007.jpg)
![Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Flow Cytometry of human p27/Kip1 on HeLa cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled p27/Kip1 Monoclonal Antibody (SX53G8).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34718-img0006.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Formalin-paraffin human colon stained with p27 MAb (SX53G8)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34718-img0004.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Formalin-fixed, paraffin-embedded human prostate Carcinoma stained with p27 Monoclonal Antibody (sx53G8)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34718-img0005.jpg)
![Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Analysis of PFA-fixed MCF-7 cells using p27/Kip1 Antibody (SX53G8) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34718-img0003.jpg)
![Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Western-Blot-NBP2-34621-img0002.jpg "Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34621-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34621-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0005.jpg)
![Western Blot: SLC26A3 Antibody [NBP1-84450] - Analysis in control (vector only transfected HEK293T lysate) and SLC26A3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SLC26A3-Antibody-Western-Blot-NBP1-84450-img0006.jpg "Western Blot: SLC26A3 Antibody [NBP1-84450] - Analysis in control (vector only transfected HEK293T lysate) and SLC26A3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: SLC26A3 Antibody [NBP1-84450] - Analysis in human colon and skeletal muscle tissues using NBP1-84450 antibody. Corresponding SLC26A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SLC26A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84450-img0007.jpg)
![Immunohistochemistry-Paraffin: SLC26A3 Antibody [NBP1-84450] - Staining of human colon shows moderate positivity in glandular cells of apical membrane.](http://images.novusbio.com/fullsize/SLC26A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84450-img0003.jpg)
![Immunohistochemistry-Paraffin: SLC26A3 Antibody [NBP1-84450] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SLC26A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84450-img0004.jpg)
![Immunohistochemistry-Paraffin: SLC26A3 Antibody [NBP1-84450] - Staining of human small intestine shows moderate to strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/SLC26A3-Antibody-Immunohistochemistry-Paraffin-NBP1-84450-img0005.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody (Competitor A) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody (Competitor A) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP3-13287-img0009.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0003.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0004.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0005.jpg)
![Western Blot: TOMM20 Antibody [NBP3-13287] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TOMM20 Antibody [NBP3-13287] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (NBP3-13287) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP3-13287-img0010.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP3-13287-img0001.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP3-13287-img0002.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-910202420122058.jpg)
![Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-910202420104450.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:615.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-91020242063371.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP3-13287] - TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-910202420122064.jpg)
![TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TOMM20 stained by TOMM20 antibody (NBP3-13287) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13287_rabbit-tomm20-pab-immunocytochemistry-immunofluorescence-2611202518204719.jpg)
![Western Blot: Serpin B5/Maspin Antibody [NBP1-87778] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MASPIN/Serpin B5 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human MASPIN/Serpin B5 Polyclonal Antibody (Catalog # NBP1-87778) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MASPIN/Serpin B5 at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Knockdown-Validated-NBP1-87778-img0020.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Serpin B5/Maspin Antibody [NBP1-87778] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MASPIN/Serpin B5 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human MASPIN/Serpin B5 Polyclonal Antibody (Catalog # NBP1-87778) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MASPIN/Serpin B5 at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Serpin B5/Maspin Antibody [NBP1-87778] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87778-img0019.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87778] - Staining of human liver.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87778-img0017.jpg)
![Western Blot: Serpin B5/Maspin Antibody [NBP1-87778] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87778-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Serpin B5/Maspin Antibody [NBP1-87778] - Analysis in human cell lines PC-3 and Caco-2 using Anti-SERPINB5 antibody. Corresponding SERPINB5 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87778-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87778] - Staining of human cerebral cortex, liver, skin and testis using Anti-SERPINB5 antibody NBP1-87778 (A) shows similar protein distribution across tissues to independent antibody NBP1-87780 (B).](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87778-img0012.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87778] - Staining of human skin.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87778-img0014.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87778] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87778-img0015.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87778] - Staining of human testis.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87778-img0016.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Immunocytochemistry-Immunofluorescence-NBP3-13664-img0003.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Immunohistochemistry-Paraffin-NBP3-13664-img0002.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0004.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0005.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0006.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-91020242011411.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-910202420115086.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-91020242021972.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-1610202419405743.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-western-blot-2611202518303816.jpg)
![Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP1-88057] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Plastin-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88057-img0012.jpg "Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP1-88057] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Plastin L Antibody [NBP1-88057] -Staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88057_rabbit-polyclonal-plastin-l-antibody-immunohistochemistry-paraffin-2282024113244..jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Analysis using Anti-ARG1 antibody NBP1-87455 (A) shows similar pattern to independent antibody NBP1-87490 (B).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87455-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Analysis using Anti-ARG1 antibody NBP1-87455 (A) shows similar pattern to independent antibody NBP1-87490 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining in human liver and pancreas tissues using anti-ARG1 antibody. Corresponding ARG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87455-img0009.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87455-img0005.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87455-img0007.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87455-img0008.jpg)
![Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-2120249371541.jpg "Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (NBP3-25455) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-21202493913.jpg)
![Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-2120249432813.jpg)
![Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-212024941491.jpg)
![Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. EPO stained by EPO antibody [HL1794] (NBP3-25455) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-212024941438.jpg)
![Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. EPO stained by EPO antibody [HL1794] (NBP3-25455) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-212024941443.jpg)
![ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1794] (NBP3-25455) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1793] (NBP3-25454) (1 ug/mL) Please notice that NBP3-25454 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25455. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-2120249355565.jpg)
![ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (NBP3-25455) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-2120249432861.jpg)
![ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1793] (NBP3-25454) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1794] (NBP3-25455) (1 ug/mL) Please notice that NBP3-25455 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25454. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-212024941445.jpg)
![Sandwich ELISA detection of recombinant (CHO cells expressed, full-length human EPO protein) using antibodies as below. Capture: EPO antibody [HL1793] (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1794] (NBP3-25455-01) (1 ug/mL](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-elisa-261120251844108.jpg)
![Western Blot: Serpin B5/Maspin Antibody [NBP1-87779] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87779-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Serpin B5/Maspin Antibody [NBP1-87779] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Western Blot: Serpin B5/Maspin Antibody [NBP1-87779] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MASPIN/Serpin B5 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human MASPIN/Serpin B5 Polyclonal Antibody (Catalog # NBP1-87779) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MASPIN/Serpin B5 at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Knockdown-Validated-NBP1-87779-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87779] - Staining of human cerebral cortex, liver, skin and testis using Anti-SERPINB5 antibody NBP1-87779 (A) shows similar protein distribution across tissues to independent antibody NBP1-87780 (B).](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87779-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Serpin B5/Maspin Antibody [NBP1-87779] - Western blot analysis in human cell lines PC-3 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87779-img0008.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87779] - Staining of human liver.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87779-img0014.jpg)
![Western Blot: Serpin B5/Maspin Antibody [NBP1-87779] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87779-img0007.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87779] - Staining of human skin.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87779-img0011.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87779] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87779-img0012.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87779] - Staining of human testis.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87779-img0013.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Rat brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0005.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0001.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0007.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Mouse brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0002.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Rat brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0003.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Mouse brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0004.jpg)
