| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49320-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-206SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47945-0.1mg | |||
| IHC-Prediluted | NBP2-48086 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56177SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56529SS | |||
| Azide and BSA Free | NBP2-80615 | |||
![Western Blot: Rab11A Antibody [NBP2-49320] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/Rab11A-Antibody-Western-Blot-NBP2-49320-img0003.jpg "Western Blot: Rab11A Antibody [NBP2-49320] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue")
![Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human stomach shows moderate cytoplasmic positivity in parietal cells.](http://images.novusbio.com/fullsize/Rab11A-Antibody-Immunohistochemistry-Paraffin-NBP2-49320-img0008.jpg)
![Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human colon shows moderate positivity in glandular cells.](http://images.novusbio.com/fullsize/Rab11A-Antibody-Immunohistochemistry-Paraffin-NBP2-49320-img0005.jpg)
![Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Rab11A-Antibody-Immunohistochemistry-Paraffin-NBP2-49320-img0006.jpg)
![Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Rab11A-Antibody-Immunohistochemistry-Paraffin-NBP2-49320-img0007.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-418_rabbit-polyclonal-neurofibromin-1-antibody-western-blot-132202618215117.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92100_rabbit-polyclonal-mat2a-antibody-western-blot-13220261739820.jpg)
![Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0017.jpg "<b>Biological Strategies Validation. </b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PKD2 antibody (lot For blotting immunoprecipitated PKD2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (A. 30 ug/lane; B. 200 mcg/IP) from HEK293 cells that were untreated or transfected with expression constructs for PKD1 and PKD3 as controls for reactivity with these homologs. Antibodies: A. Affinity purified rabbit anti- NB100-636 used at the indicated oncentrations for WB. B. Affinity purified rabbit anti- NB100-636 and NB100-2299 used at 2 ug/200 mcg lysate for IP. Immunoprecipitated PKD2 was detected by WB using anti-PKD2 antibody NB100-2299 at 1 ug/ml. Detection: Chemiluminescence with a 10 second exposure.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunoprecipitation-NB100-636-img0003.jpg)
![Western Blot: AF9 Antibody [NB100-1565] - Detection of Human AF9 on HeLa whole cell lysates using NB100-1565. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies NB100-1564, NB100-1566 and another AF9 Ab.](http://images.novusbio.com/fullsize/AF9-Antibody-Western-Blot-NB100-1565-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AF9 Antibody [NB100-1565] - Detection of Human AF9 on HeLa whole cell lysates using NB100-1565. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies NB100-1564, NB100-1566 and another AF9 Ab.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AF9 Antibody [NB100-1565] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AF9 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/AF9-Antibody-Immunohistochemistry-NB100-1565-img0003.jpg)
![Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Knockdown-Validated-NB500-206-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Western-Blot-NB500-206-img0002.jpg)
![Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Simple Western lane view shows a specific band for Caspase 7 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Simple-Western-NB500-206-img0003.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Western Blot: p38 alpha Antibody [NBP2-19662] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Western-Blot-NBP2-19662-img0010.jpg "Western Blot: p38 alpha Antibody [NBP2-19662] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded human lung adenocarcinoma. p38 antibody [N1C3-2] diluted at 1:250.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0003.jpg)
![Immunohistochemistry-Paraffin: p38 Antibody [NBP2-19662] - Paraffin-embedded rat brain. p38 MAPK antibody [N1C3-2] diluted at 1:500.](http://images.novusbio.com/fullsize/p38-Antibody-Immunohistochemistry-Paraffin-NBP2-19662-img0002.jpg)
![p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-immunocytochemistry-immunofluorescence-261120251995418.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (NBP2-19662) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19662_rabbit-polyclonal-p38-alpha-antibody-western-blot-261120251837242.jpg)
![Western Blot: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Antibody (KRT19/799).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Western-Blot-NBP2-44826-img0009.jpg "Western Blot: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Antibody (KRT19/799).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - ICC/IF analysis of MCF-7 cells labeling Cytokeratin 19 with Conjugated with CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunofluorescence-NBP2-44826-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0005.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Flow Cytometric Analysis of MeOH-fixed MCF-7 cells using Cytokeratin 19 Antibody (KRT19/799) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Flow-Cytometry-NBP2-44826-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Bladder Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799) [NBP2-44826] - Human Ovarian Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-Immunohistochemistry-Paraffin-NBP2-44826-img0004.jpg)
![Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177] - Formalin-fixed, paraffin embedded, normal human mammary epithelial tissue using th antibody. A-C: Strong TRAF4 staining is seen in the luminal epithelial cells of the interlobular terminal ducts (acini). However, there is absence of TRAF4 immunostaining in the myoepithelial cells along the basement membrane. B and C: higher magnifications of A. Data courtesy of Krajewska et al.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunohistochemistry-Paraffin-NB100-56177-img0008.jpg "Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177] - Formalin-fixed, paraffin embedded, normal human mammary epithelial tissue using th antibody. A-C: Strong TRAF4 staining is seen in the luminal epithelial cells of the interlobular terminal ducts (acini). However, there is absence of TRAF4 immunostaining in the myoepithelial cells along the basement membrane. B and C: higher magnifications of A. Data courtesy of Krajewska et al.")
![Immunoprecipitation: TRAF-4 Antibody [NB100-56177] - The NB100-56177 TRAF4 antibody specifically immunoprecipitates TRAF4. Top: TRAFs 2,3,4,5, and 6 were in vitro translated in the presence of 35S-methionine, and immunoprecipiated using the TRAF4 antibody. The TRAF4 antibody immunoprecipitated only TRAF4 and did not recognize TRAFs 2, 3, 5, or 6. A reticulocyte primed with an empty pcDNA plasmid was used as a negative control. Bottom: SDS-PAGE visualization of the in vitro translated TRAFs. Data courtesy of Krajewska et al.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunoprecipitation-NB100-56177-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ube2L3 Antibody [NBP2-20786] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Ube2L3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20786-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ube2L3 Antibody [NBP2-20786] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Western Blot: Ube2L3 Antibody [NBP2-20786] - Sample (30 ug of whole cell lysate) A: JurKat B: Hela 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Ube2L3-Antibody-Western-Blot-NBP2-20786-img0001.jpg)
![Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20786_rabbit-polyclonal-ubch7-ube2l3-antibody-910202420122077.jpg)
![Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20786_rabbit-polyclonal-ubch7-ube2l3-antibody-91020242012208.jpg)
![Immunohistochemistry: UbcH7/UBE2L3 Antibody [NBP2-20786] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-20786_rabbit-polyclonal-ubch7-ube2l3-antibody-91020242015724.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20786_rabbit-polyclonal-ubch7-ube2l3-antibody-western-blot-2611202518303817.jpg)
![Western Blot: NDUFA10 Antibody [NBP1-32146] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFA10 antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/NDUFA10-Antibody-Western-Blot-NBP1-32146-img0004.jpg "Western Blot: NDUFA10 Antibody [NBP1-32146] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFA10 antibody [N1C3] diluted at 1:500.")
![Immunohistochemistry-Paraffin: NDUFA10 Antibody [NBP1-32146] - Human colon , using NDUFA10 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NDUFA10-Antibody-Immunohistochemistry-Paraffin-NBP1-32146-img0002.jpg)
![Western Blot: NDUFA10 Antibody [NBP1-32146] - Sample(30 ug of whole cell lysate)A:H1299 B:Raji 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/NDUFA10-Antibody-Western-Blot-NBP1-32146-img0003.jpg)
![NDUFA10 antibody [N1C3] detects NDUFA10 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.Green: NDUFA10 protein stained by NDUFA10 antibody [N1C3] (NBP1-32146) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32146_rabbit-polyclonal-ndufa10-antibody-immunocytochemistry-immunofluorescence-141120251843322.jpg)
![Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0008.jpg "Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0009.jpg)
![Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) [NB100-56529] - Human tonsil stained with Caspase-7 antibody at 5 ug/ml. Novus' human tonsil tissue slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-25B881-1-Immunohistochemistry-Paraffin-NB100-56529-img0007.jpg)