| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71528 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77321SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71615 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77257-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76695-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73438 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34921-1mg | |||
| Carrier-Free | NBP2-34921-100ug | |||
| Carrier-Free | NBP2-34921-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61702-0.025ml | |||
![Western Blot: AKT3 Antibody (OTI9B2) [NBP1-47657] - Analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/AKT3-Antibody-OTI9B2-Western-Blot-NBP1-47657-img0006.jpg "Western Blot: AKT3 Antibody (OTI9B2) [NBP1-47657] - Analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.")
![Immunocytochemistry/Immunofluorescence: AKT3 Antibody (9B2) [NBP1-47657] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Immunocytochemistry-Immunofluorescence-NBP1-47657-img0005.jpg)
![Immunohistochemistry-Paraffin: AKT3 Antibody (9B2) [NBP1-47657] - Staining of paraffin-embedded Human lung tissue using anti-AKT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Immunohistochemistry-Paraffin-NBP1-47657-img0004.jpg)
![Flow Cytometry: AKT3 Antibody (9B2) [NBP1-47657] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AKT3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Flow-Cytometry-NBP1-47657-img0002.jpg)
![Western Blot: AKT3 Antibody (9B2) [NBP1-47657] - AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Western-Blot-NBP1-47657-img0001.jpg)
![Western Blot: AKT3 Antibody (9B2) [NBP1-47657] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Western-Blot-NBP1-47657-img0003.jpg)
![Western Blot: DDIT4 Antibody [NBP1-77321] - siRNA knockdown of the DDIT4 antiproliferative responses to 1,25(OH)2 D3RMCs were transfected with negative control siRNA orDDIT4-specific siRNA in the presence or absence of 1,25(OH) 2 D3 . (C) DDIT4 protein expression levels were detected by Western blot. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23063) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Knockdown-Validated-NBP1-77321-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DDIT4 Antibody [NBP1-77321] - siRNA knockdown of the DDIT4 antiproliferative responses to 1,25(OH)2 D3RMCs were transfected with negative control siRNA orDDIT4-specific siRNA in the presence or absence of 1,25(OH) 2 D3 . (C) DDIT4 protein expression levels were detected by Western blot. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23063) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: DDIT4 Antibody [NBP1-77321] - 1,25(OH)2 D3 modulates RMCs proliferation induced by high glucose via the DDIT4/mTOR signaling pathwayRMCs were cultured for 48 h in the presence or absence of 1,25(OH)2 D3. Cell lysates were subjected to Western blot analysis to measure the levels of DDIT4. LG: 5.5 mM glucose; Man: 5.5 mM glucose + 24.5 mM mannitol; HG: 30 mM glucose. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23063) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-77321-img0010.jpg)
![Western Blot: DDIT4 Antibody [NBP1-77321] - Human kidney tissue lysate with DDIT4 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-77321-img0006.jpg)
![Immunohistochemistry: DDIT4 Antibody - BSA Free [NBP1-77321] - Immunohistochemistry of DDIT4 in mouse kidney tissue with DDIT4 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77321_rabbit-polyclonal-ddit4-antibody-271120242016958.jpg)
![Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody - BSA Free [NBP1-77321] - Immunofluorescence of DDIT4 in mouse kidney tissue with DDIT4 antibody at 20 ug/mL.Red: DDIT4 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77321_rabbit-polyclonal-ddit4-antibody-2711202419592030.gif)
![Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Western-Blot-NBP2-02627-img0010.jpg "Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunocytochemistry-Immunofluorescence-NBP2-02627-img0009.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Human liver tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0008.jpg)
![Flow Cytometry: IGF2BP2 Antibody (3F9) [NBP2-02627] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Flow-Cytometry-NBP2-02627-img0002.jpg)
![Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Western-Blot-NBP2-02627-img0003.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0004.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0005.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0006.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0007.jpg)
![Flow Cytometry: IGF2BP2 Antibody (3F9) [NBP2-02627] - Analysis of Jurkat cells, using anti-IGF2BP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Flow-Cytometry-NBP2-02627-img0001.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. Representative immunoblots and quantification of indicated proteins from epidermal lysates of 7-8-week old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test). Representative immunoblots and quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7-8-week-old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test; *p?less than or equal ?0.05; **p?less than or equal ?0.01; ***p?less than or equal ?0.001) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0012.jpg "Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. Representative immunoblots and quantification of indicated proteins from epidermal lysates of 7-8-week old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test). Representative immunoblots and quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7-8-week-old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test; *p?less than or equal ?0.05; **p?less than or equal ?0.01; ***p?less than or equal ?0.001) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Abdominal Aortic Aneurysm (AAA) Tissues (Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Immunohistochemistry-Paraffin-NBP1-77257-img0011.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Western blot analysis of IL-23 in Raji lysate with IL-23 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0003.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 30 ng of human IL-23 recombinant protein.Antibodies: IL-23 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0009.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 20ug of lysates per lane.Antibodies: IL-23, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0010.jpg)
![Immunohistochemistry: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Staining of human pancreas tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Immunohistochemistry-NBP1-77257-img0005.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. a qRT-PCR analysis of indicated genes from back skin RNA of con & ko at indicated ages (n > = 3/3, two-tailed unpaired t-test). All normalized to GAPDH. b Representative toluidine blue staining for barrier defect in con & ko mice at indicated ages (P: E15.5 embryos used as positive controls; 2 days (2d) n: 6/5, 6 days(6d) n: 4/5, 3 independent experiments). c Representative immunofluorescence staining & quantification of IL-23p19 of con & ko back skin at indicated ages (n: 3/3, two-tailed paired t-test). Scale bar: 100 µm. d Representative sorting strategy of CD45+, alpha 6H, & alpha 6L cells from con & ko epidermal cells & qRT-PCR analysis of IL-23A & IL-17A expression in sorted cells (n: 3/3, mean ± SD, two-tailed unpaired t-test, 3 independent sortings). e Representative immunoblots & quantification of indicated proteins from epidermal lysates of 7–8-week old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test). f Representative immunoblots & quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7–8-week-old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-31020241616094.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-271120241923687.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunocytochemical analysis of Raji cells using anti-IL-23A/IL-23 P19 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-2711202419562127.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-2711202419392713.gif)
![Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation in Human Raji Cells. Loading: 15 ug of lysates per lane.Antibodies: IL-23A/IL-23 P19 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Absence of blocking peptideLane B: Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-2711202419381757.jpg)
![Western Blot: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Western-Blot-NBP2-45872-img0011.jpg "Western Blot: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.")
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human placenta tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0010.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0001.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0002.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0003.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0004.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0005.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0006.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0007.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0008.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human testicular cancer tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0009.jpg)
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0011.jpg "Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0004.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human TWEAK recombinant protein per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL and Lane 4: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18 kDa.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0009.jpg)
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Pancreas Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0010.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-TWEAK/TNFSF12 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420115594.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TWEAK/TNFSF12 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420272466.gif)
![Immunocytochemistry/ Immunofluorescence: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TWEAK/TNFSF12 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202419514021.gif)
![Western Blot: PKC epsilon Antibody (4G8) [NBP2-03620] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PKC epsilon monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Western-Blot-NBP2-03620-img0010.jpg "Western Blot: PKC epsilon Antibody (4G8) [NBP2-03620] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PKC epsilon monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKC epsilon.](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Immunocytochemistry-Immunofluorescence-NBP2-03620-img0009.jpg)
![Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Human prostate tissue using anti-PKC epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-03620-img0008.jpg)
![Flow Cytometry: PKC epsilon Antibody (4G8) [NBP2-03620] - Analysis of Hela cells, using anti-PKC epsilon antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Flow-Cytometry-NBP2-03620-img0001.jpg)
![Western Blot: PKC epsilon Antibody (4G8) [NBP2-03620] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKC epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKC epsilon.](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Western-Blot-NBP2-03620-img0002.jpg)
![Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PKC epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-03620-img0003.jpg)
![Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PKC epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-03620-img0004.jpg)
![Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PKC epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-03620-img0005.jpg)
![Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Human lymph node tissue using anti-PKC epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-03620-img0006.jpg)
![Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Human lymphoma tissue using anti-PKC epsilon mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKC-epsilon-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-03620-img0007.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-00153-img0005.jpg "Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00153-img0006.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-00153-img0003.jpg)
![Flow Cytometry: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Flow-Cytometry-NBP1-00153-img0007.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of A431 (A) and (1ug/ml) NIH3T3(B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-00153-img0004.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-00153-img0002.jpg)
![Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB110-40660-img0002.jpg "Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB110-40660] - Staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Antibody at 5 ug/mL. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40660-img0001.jpg)
![Western Blot: Cdk9 Antibody (1C4E3) [NBP3-15345] - Western blot analysis of extracts of various cell lines, using Cdk9 Rabbit mAb (NBP3-15345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Western-Blot-NBP3-15345-img0006.jpg "Western Blot: Cdk9 Antibody (1C4E3) [NBP3-15345] - Western blot analysis of extracts of various cell lines, using Cdk9 Rabbit mAb (NBP3-15345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunofluorescence analysis of NIH-3T3 cells using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunocytochemistry-Immunofluorescence-NBP3-15345-img0005.jpg)
![Immunohistochemistry-Paraffin: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunohistochemistry of paraffin-embedded mouse brain using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunohistochemistry-Paraffin-NBP3-15345-img0003.jpg)
![Chromatin Immunoprecipitation Sequencing: Cdk9 Antibody (1C4E3) [NBP3-15345] - Chromatin immunoprecipitations were performed with cross-linked chromatin from DLD-1 cells and Cdk9 Rabbit mAb (NBP3-15345). The ChIP sequencing results indicate the enrichment pattern of Cdk9 in selected genomic region and representative gene loci (e.g. RAC1, GAPDH and ACTB), as shown in figure.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Chromatin-Immunoprecipitation-Sequencing-NBP3-15345-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunofluorescence analysis of HeLa cells using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunocytochemistry-Immunofluorescence-NBP3-15345-img0004.jpg)
![Immunohistochemistry-Paraffin: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunohistochemistry of paraffin-embedded rat ovary using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunohistochemistry-Paraffin-NBP3-15345-img0001.jpg)
![Immunohistochemistry-Paraffin: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunohistochemistry of paraffin-embedded human esophageal using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunohistochemistry-Paraffin-NBP3-15345-img0002.jpg)
![Immunoprecipitation: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Cdk9 antibody (NBP3-15345). Western blot was performed from the immunoprecipitate using Cdk9 antibody (NBP3-15345) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunoprecipitation-NBP3-15345-img0007.jpg)
![Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15345_rabbit-cdk9-mab-1c4e3-52202517192986.jpg)
![Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15345_rabbit-cdk9-mab-1c4e3-52202517235768.jpg)
![Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15345_rabbit-cdk9-mab-1c4e3-52202517202911.jpg)
![Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15345_rabbit-cdk9-mab-1c4e3-5220251718457.jpg)
![Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15345_rabbit-cdk9-mab-1c4e3-52202517273599.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk9 Antibody (1C4E3) [Cdk9] - Western blot analysis of lysates from wild type(WT) and Cdk9 knockout (KO) 293T cells, using [KO Validated] Cdk9 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15345_rabbit-cdk9-mab-1c4e3-5220251718990.jpg)
![Immunocytochemistry/ Immunofluorescence: Cdk9 Antibody (1C4E3) [Cdk9] - Confocal imaging of U-2 OS cells using [KO Validated] Cdk9 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-15345_rabbit-cdk9-mab-1c4e3-5220251719295.jpg)
![Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15345_rabbit-cdk9-mab-1c4e3-52202517213565.jpg)
![SDS-Page: Human FGF basic/FGF2 Protein [NBP2-34921]](http://images.novusbio.com/fullsize/Human-FGF-basic-FGF2-Protein-SDS-Page-NBP2-34921-img0001.jpg "SDS-Page: Human FGF basic/FGF2 Protein [NBP2-34921]")
![Western Blot: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis using BCL11B mAb against human BCL11B (AA: 1-150) recombinant protein. (Expected MW is 42.3 kDa)](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Western-Blot-NBP2-61702-img0006.jpg "Western Blot: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis using BCL11B mAb against human BCL11B (AA: 1-150) recombinant protein. (Expected MW is 42.3 kDa)")
![Immunocytochemistry/Immunofluorescence: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of Hela cells using BCL11B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Immunocytochemistry-Immunofluorescence-NBP2-61702-img0003.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of stomach cancer tissues using BCL11B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Immunohistochemistry-NBP2-61702-img0005.jpg)
![Flow Cytometry: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of Jurkat cells using BCL11B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Flow-Cytometry-NBP2-61702-img0010.jpg)
![Western Blot: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis using BCL11B mAb against HEK293 (1) and BCL11B (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Western-Blot-NBP2-61702-img0007.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of colon cancer tissues using BCL11B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Immunohistochemistry-NBP2-61702-img0004.jpg)
![Flow Cytometry: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of Hela cells using BCL11B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Flow-Cytometry-NBP2-61702-img0002.jpg)
![ELISA: BCL11B Antibody (1F8G8) [NBP2-61702] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-ELISA-NBP2-61702-img0001.jpg)