| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61746-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52513-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22205-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35076-1mg | |||
| Carrier-Free | NBP2-35076-100ug | |||
| Carrier-Free | NBP2-35076-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27152-0.025ml | |||
![Western Blot: USP9x Antibody (7T0S2) [NBP3-16884] - Western blot analysis of extracts of various cell lines, using USP9x Rabbit mAb (NBP3-16884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Western-Blot-NBP3-16884-img0004.jpg "Western Blot: USP9x Antibody (7T0S2) [NBP3-16884] - Western blot analysis of extracts of various cell lines, using USP9x Rabbit mAb (NBP3-16884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (7T0S2) [NBP3-16884] - Immunofluorescence analysis of NIH-3T3 cells using USP9x Rabbit mAb (NBP3-16884) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Immunocytochemistry-Immunofluorescence-NBP3-16884-img0003.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (7T0S2) [NBP3-16884] - Immunohistochemistry of paraffin-embedded mouse testis using USP9x Rabbit mAb (NBP3-16884) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Immunohistochemistry-Paraffin-NBP3-16884-img0002.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (7T0S2) [NBP3-16884] - Immunohistochemistry of paraffin-embedded rat testis using USP9x Rabbit mAb (NBP3-16884) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Immunohistochemistry-Paraffin-NBP3-16884-img0001.jpg)
![Western Blot: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis using GRIN2B mAb against human GRIN2B (AA: extra 27-163) recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Western-Blot-NBP2-61746-img0005.jpg "Western Blot: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis using GRIN2B mAb against human GRIN2B (AA: extra 27-163) recombinant protein. (Expected MW is 41 kDa)")
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis of SK-N-SH cells using GRIN2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Immunocytochemistry-Immunofluorescence-NBP2-61746-img0004.jpg)
![Flow Cytometry: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis of SK-N-SH cells using GRIN2B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Flow-Cytometry-NBP2-61746-img0002.jpg)
![Western Blot: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis using GRIN2B mAb against HEK293 (1) and GRIN2B (AA: extra 27-163)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Western-Blot-NBP2-61746-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis of Hela cells using GRIN2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Immunocytochemistry-Immunofluorescence-NBP2-61746-img0003.jpg)
![ELISA: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-ELISA-NBP2-61746-img0001.jpg)
![Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against human mTOR (AA: 2311-2529) recombinant protein. (Expected MW is 50.8 kDa)](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Western-Blot-NBP2-52513-img0003.jpg "Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against human mTOR (AA: 2311-2529) recombinant protein. (Expected MW is 50.8 kDa)")
![Immunohistochemistry-Paraffin: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis of endometrial cancer tissues using mTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Immunohistochemistry-NBP2-52513-img0002.jpg)
![Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against HEK293 (1) and mTOR (AA: 2311-2529)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Western-Blot-NBP2-52513-img0004.jpg)
![Immunohistochemistry-Paraffin: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis of brain tissues using mTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Immunohistochemistry-NBP2-52513-img0001.jpg)
![ELISA: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-ELISA-NBP2-52513-img0005.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Western blot analysis using S6K S6K mAb against human RPS6KB1 (AA: 295-524) recombinant protein. (Expected MW is 59 kDa)](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-Western-Blot-NBP2-22205-img0005.jpg "Western Blot: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Western blot analysis using S6K S6K mAb against human RPS6KB1 (AA: 295-524) recombinant protein. (Expected MW is 59 kDa)")
![Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Immunohistochemical analysis of paraffin-embedded muscle tissues using S6K mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-Immunohistochemistry-Paraffin-NBP2-22205-img0003.jpg)
![Flow Cytometry: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Flow cytometric analysis of Jurkat cells using S6K mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-Flow-Cytometry-NBP2-22205-img0001.jpg)
![Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using S6K mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-Immunohistochemistry-Paraffin-NBP2-22205-img0002.jpg)
![ELISA: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-ELISA-NBP2-22205-img0004.jpg)
![SDS-Page: Human TNF-alpha Protein [NBP2-35076]](http://images.novusbio.com/fullsize/Human-TNF-alpha-Protein-SDS-Page-NBP2-35076-img0001.jpg "SDS-Page: Human TNF-alpha Protein [NBP2-35076]")
![Western Blot: MEK2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72647] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2.](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Azide-and-BSA-Free-Western-Blot-NBP2-72647-img0002.jpg "Western Blot: MEK2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72647] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2.")
![Immunocytochemistry/Immunofluorescence: MEK2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72647] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MEK2.](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72647-img0003.jpg)
![Western Blot: MEK2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72647] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Azide-and-BSA-Free-Western-Blot-NBP2-72647-img0001.jpg)
![Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-644-img0002.jpg "Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.")
![Simple Western: PINK1 Antibody [NB100-644] - Simple Western lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~56 kDa. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NB100-644-img0003.jpg)
![Western Blot: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of ULK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: MCF-7 cell lysate Lane 3: mouse spleen tissue lysate](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Western-Blot-NBP2-66765-img0005.jpg "Western Blot: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of ULK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: MCF-7 cell lysate Lane 3: mouse spleen tissue lysate")
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded rat skeleton muscle tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0004.jpg)
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded human colon cancer tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0001.jpg)
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded human spleen tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0002.jpg)
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded mouse brain tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0003.jpg)
![Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-278202415212422.jpg "Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32649, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29820241420992.jpg)
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32649) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32649) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-298202414333659.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29202410234263.jpg)
![Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-278202415212420.jpg "Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32650) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-29820241420994.jpg)
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32650, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-298202414262675.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PS02-10) [NBP3-32650] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-2920241192341.jpg)
![Western Blot: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis using WDFY3 mAb against HEK293 (1) and WDFY3 (AA: 3277-3526)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-10420249181942.jpg%3Fv=1666704387 "Western Blot: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis using WDFY3 mAb against HEK293 (1) and WDFY3 (AA: 3277-3526)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis of Hela cells using WDFY3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-104202491626.jpg%3Fv=1666704387)
![Immunohistochemistry: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis of paraffin-embedded bladder cancer tissues using WDFY3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-1042024916227.jpg%3Fv=1666704387)
![ELISA: WDFY3 Antibody (2F7C2) [NBP3-27152] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-1042024920241.jpg%3Fv=1666704387)
![Western Blot: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis using WDFY3 mAb against human WDFY3 (AA: 3277-3526) recombinant protein. (Expected MW is 54.2 kDa)](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-104202491707.jpg%3Fv=1666704387)
![Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0002.jpg "Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.")
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Staining of PFA fixed HEK293 cells labelled with hamster anti-Atg9 (Left) and rabbit anti-Atg9.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Immunofluorescence-NBP2-50109-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of siRNA knockdown of human Atg9 (si) in Hela and Hek293 cells compared to siControl siRNA (siC) reveals specicity of hamster anti-Atg9 antibody.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0003.jpg)