| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74854 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36445-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77242-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76377-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77169-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61659-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-02952-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89844-50ul | |||
![Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Western-Blot-NB100-293-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of extracts (35ug) from 9 different cell line (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/VPS28-Antibody-OTI1A8-Western-Blot-NBP2-45412-img0003.jpg "Western Blot: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of extracts (35ug) from 9 different cell line (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VPS28-Antibody-OTI1A8-Immunohistochemistry-NBP2-45412-img0002.jpg)
![Western Blot: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28.](http://images.novusbio.com/fullsize/VPS28-Antibody-OTI1A8-Western-Blot-NBP2-45412-img0004.jpg)
![Immunofluorescence: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY VPS28.](http://images.novusbio.com/fullsize/VPS28-Antibody-OTI1A8-Immunofluorescence-NBP2-45412-img0001.jpg)
![Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target of rapamycin (mTOR), ULK1, p-GSK3Beta (Ser9), GSK3Beta in extracts of whole brain tissue were detected by WB. Representative Western blots are presented in panels, and quantification of the results is shown as the mean values +- SD in panels (B,D,F,H). *p less than 0.05, **p less than 0.01, and ***p less than 0.001 between two groups. One-way analysis of variance/Newman-Keuls test was performed for all WB data. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fnagi.2020.00047/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATG14-Antibody-Western-Blot-NBP2-36445-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target of rapamycin (mTOR), ULK1, p-GSK3Beta (Ser9), GSK3Beta in extracts of whole brain tissue were detected by WB. Representative Western blots are presented in panels, and quantification of the results is shown as the mean values +- SD in panels (B,D,F,H). *p less than 0.05, **p less than 0.01, and ***p less than 0.001 between two groups. One-way analysis of variance/Newman-Keuls test was performed for all WB data. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fnagi.2020.00047/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - Analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ATG14-Antibody-Western-Blot-NBP2-36445-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG14 Antibody - BSA Free [NBP2-36445] - Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-36445_rabbit-polyclonal-atg14-antibody-2711202420285856.jpg)
![Western Blot: PIST Antibody [NBP1-77242] - Analysis in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/mL.](http://images.novusbio.com/fullsize/PIST-Antibody-Western-Blot-NBP1-77242-img0004.jpg "Western Blot: PIST Antibody [NBP1-77242] - Analysis in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/mL.")
![Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunohistochemistry of PIST in human testis tissue with PIST antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-2711202419531635.gif)
![Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-2711202419463455.jpg)
![Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunofluorescence of PIST in human testis tissue with PIST antibody at 20 ug/ml.Green: PIST Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-2711202420195363.gif)
![Immunocytochemistry/ Immunofluorescence: PIST Antibody - BSA Free [NBP1-77242] - Immunofluorescence of PIST in Rat Colon cells with PIST antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-271120242013170.jpg)
![Western Blot: IRGM Antibody [NBP1-76377] - Analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/IRGM-Antibody-Western-Blot-NBP1-76377-img0005.jpg "Western Blot: IRGM Antibody [NBP1-76377] - Analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry-Paraffin: IRGM Antibody [NBP1-76377] - Staining of CD4 in human thymus tissue with CD4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IRGM-Antibody-Immunohistochemistry-Paraffin-NBP1-76377-img0003.jpg)
![Immunohistochemistry: IRGM Antibody [NBP1-76377] - Staining of IRGM in Human Brain tissue with IRGM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/IRGM-Antibody-Immunofluorescence-NBP1-76377-img0002.jpg)
![Immunohistochemistry: IRGM Antibody - BSA Free [NBP1-76377] - Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76377_rabbit-polyclonal-irgm-antibody-2711202420141919.gif)
![Immunohistochemistry: IRGM Antibody - BSA Free [NBP1-76377] - Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76377_rabbit-polyclonal-irgm-antibody-2711202420195388.gif)
![Western Blot: ATG9B Antibody [NBP1-77169] - HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ATG9B-Antibody-Western-Blot-NBP1-77169-img0001.jpg "Western Blot: ATG9B Antibody [NBP1-77169] - HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] - Immunofluorescence of ATG9B in Hela cells with ATG9B antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77169_rabbit-polyclonal-atg9b-antibody-2711202419313264.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] - Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-77169_rabbit-polyclonal-atg9b-antibody-2711202420212356.jpg)
![Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (15 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-BLR103H-Western-Blot-NBP3-14695-img0002.jpg "Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (15 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: GAPDH Antibody (BLR103H) [NBP3-14695] - Rabbit anti-GAPDH recombinant monoclonal antibody. Secondary HRP-conjugated goat anti-rabbit IgG. Substrate:DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-BLR103H-Immunohistochemistry-NBP3-14695-img0003.jpg)
![Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (50 ug) from HeLa, Jurkat, RKO, U2OS, GaMG, LNCaP, MCF-7, K-562, HEK293T, and Hep-G2 cells prepared using NETN lysis buffer. Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/GAPDH-Antibody-BLR103H-Western-Blot-NBP3-14695-img0001.jpg)
![ICC/IF-GAPDH Antibody (BLR103H)-NBP3-14695-: Rabbit anti-GAPDH recombinant monoclonalantibody [BLR103H]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14695_rabbit-gapdh-mab-immunocytochemistry-immunofluorescence-199202311112..jpg)
![Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Western-Blot-NBP2-61659-img0004.jpg "Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Immunocytochemistry-Immunofluorescence-NBP2-61659-img0003.jpg)
![Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Raji cells using NF-M mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Flow-Cytometry-NBP2-61659-img0007.jpg)
![Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 779-916)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Western-Blot-NBP2-61659-img0005.jpg)
![Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Flow-Cytometry-NBP2-61659-img0002.jpg)
![ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-ELISA-NBP2-61659-img0001.jpg)
![ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-ELISA-NBP2-61659-img0006.jpg)
![Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of HeLa cells, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MEK2-Antibody-Western-Blot-NBP3-02952-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of HeLa cells, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MEK2 Antibody-Western Blot-NBP3-02952-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02952_rabbit-polyclonal-mek2-antibody-52202519302962.jpg)
![Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02952_rabbit-polyclonal-mek2-antibody-52202519294585.jpg)
![Western Blot: PIST Antibody (2B1P9) [NBP3-15280] - Western blot analysis of extracts of various cell lines, using PIST Rabbit mAb (NBP3-15280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PIST-Antibody-2B1P9-Western-Blot-NBP3-15280-img0004.jpg "Western Blot: PIST Antibody (2B1P9) [NBP3-15280] - Western blot analysis of extracts of various cell lines, using PIST Rabbit mAb (NBP3-15280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: PIST Antibody (2B1P9) [NBP3-15280] - Immunofluorescence analysis of NIH-3T3 cells using PIST Rabbit mAb (NBP3-15280) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PIST-Antibody-2B1P9-Immunocytochemistry-Immunofluorescence-NBP3-15280-img0003.jpg)
![Immunohistochemistry-Paraffin: PIST Antibody (2B1P9) [NBP3-15280] - Immunohistochemistry of paraffin-embedded human thyroid cancer using PIST Rabbit mAb (NBP3-15280) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PIST-Antibody-2B1P9-Immunohistochemistry-Paraffin-NBP3-15280-img0001.jpg)
![Western Blot: PIST Antibody (2B1P9) [NBP3-15280] - Western blot analysis of extracts of Mouse lung, using PIST Rabbit mAb (NBP3-15280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/PIST-Antibody-2B1P9-Western-Blot-NBP3-15280-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PIST Antibody (2B1P9) [NBP3-15280] - Immunofluorescence analysis of C6 cells using PIST Rabbit mAb (NBP3-15280) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PIST-Antibody-2B1P9-Immunocytochemistry-Immunofluorescence-NBP3-15280-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0002.jpg "Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000).")
![<b>Biological Strategies Validation. </b>Flow Cytometry: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1(CD107a) on Jurkat cells. Cells were treated, stained with purified anti-Human LAMP1(CD107a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Flow-Cytometry-NBP2-89844-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human placenta with rabbit monoclonal antibody (1:1000). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0001.jpg)
![LAMP-1/CD107a was immunoprecipitated using NBP2-89844 at a 1:100 dilution and 60ug Immunomagnetic beads Protein A/G and Goat Anti-Rabbit IgG (H+L)[HRP] as a secondary antibody at 1/10000 dilution: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg Daudi Whole Cell Lysate. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 120 kDa](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-4520231646141.png)
![<b>Genetic Strategies Validation. </b>Western Blot: LAMP-1/CD107a Antibody (107) [NBP2-89844] - NBP2-89844 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: LAMP-1/CD107a knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 120 kDa. Observed band size: 100-130 kDa](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-136202422345046.png)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89844 antibody (1:300). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor(R) 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-235202514492341.png)
![Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Western-Blot-NBP3-15310-img0003.jpg "Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD133 Rabbit mAb (NBP3-15310) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Immunohistochemistry-Paraffin-NBP3-15310-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts from normal (control) and CD133 knockout (KO) HCT116 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Knockout-Validated-NBP3-15310-img0001.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [CD133] - Western blot analysis of lysates from HT-29 cells, using CD133 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517192943.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-522025172608.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517251995.jpg)