| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-97231 | |||
| FITC | NBP1-97227 | |||
| HRP | NBP1-97241 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-75344 | |||
| DyLight 550 | NBP1-76090 | |||
| DyLight 594 | NBP1-76091 | |||
| FITC | NBP1-75345 | |||
| HRP | NBP1-75346 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-62015B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25959 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-72623 | |||
| Biotin | NBP1-73430 | |||
| FITC | NBP1-73234 | |||
| HRP | NBP1-73529 | |||
| Rhodamine | NBP1-73232 | |||
| Texas Red | NBP1-73315 | |||
![SDS-Page: Rabbit IgG, Fc Native Protein [NBP1-97061] - SDS-PAGE of Rabbit IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Fc-Native-Protein-SDS-Page-NBP1-97061-img0003.jpg "SDS-Page: Rabbit IgG, Fc Native Protein [NBP1-97061] - SDS-PAGE of Rabbit IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.")
![Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Western Blot of Goat anti-Rabbit IgG Fc Secondary antibody.Lane 1: Rabbit F(c).Load: 50 ng per lane.Primary antibody: Rabbit F(c) antibody at 1:1,000 overnight at 4C.Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:20,000 for 30 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 25 kDa, 25 kDa for Rabbit F(c).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Western-Blot-NBP2-60661-img0013.jpg "Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Western Blot of Goat anti-Rabbit IgG Fc Secondary antibody.Lane 1: Rabbit F(c).Load: 50 ng per lane.Primary antibody: Rabbit F(c) antibody at 1:1,000 overnight at 4C.Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:20,000 for 30 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 25 kDa, 25 kDa for Rabbit F(c).")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Immunocytochemistry/Immunofluorescence: [DyLight 488] [NBP1-72943]](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60661-img0007.jpg)
![Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image using the Rhodamine form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Western-Blot-NBP2-60661-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Fluorophore-linked immunosorbent assay: Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0008.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0009.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0010.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0011.jpg)
![Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody (Pre-adsorbed) [NBP2-60663] - Western Blot of Goat anti-Rabbit IgG Fc Secondary antibody (Pre-adsorbed). Lane 1: Rabbit Fc. Load: 100 ng per lane.Primary antibody: Rabbit Fc antibody at 1:1,000 for overnight at 4C.Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:20,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 28 kDa, 28 kDa for Rabbit Fc. Other band(s): Rabbit Fc splice variants and isoforms.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60663-img0005.jpg "Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody (Pre-adsorbed) [NBP2-60663] - Western Blot of Goat anti-Rabbit IgG Fc Secondary antibody (Pre-adsorbed). Lane 1: Rabbit Fc. Load: 100 ng per lane.Primary antibody: Rabbit Fc antibody at 1:1,000 for overnight at 4C.Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:20,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 28 kDa, 28 kDa for Rabbit Fc. Other band(s): Rabbit Fc splice variants and isoforms.")
![Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody (Pre-adsorbed) [NBP2-60663] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60663-img0001.jpg)
![Dot Blot: Goat anti-Rabbit IgG Fc Secondary Antibody (Pre-adsorbed) [NBP2-60663]](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP2-60663-img0002.jpg)
![ELISA: Goat anti-Rabbit IgG Fc Secondary Antibody (Pre-adsorbed) [NBP2-60663] - ELISA results of purified Goat anti-Rabbit IgG Fc Secondary antibody (Pre-adsorbed) tested against purified RabbitIgG Fc (Red Line), Rabbit IgG (Green Line), and Human IgG (Blue Line). Each well was coated in duplicate with 1.0 ug of antigen RabbitIgG Fc, Rabbit IgG, and Human IgG. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Donkey Anti-Goat IgG HRP, and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Pre-adsorbed-ELISA-NBP2-60663-img0006.jpg)
![Western Blot: IgG Fc Antibody (RMG02) [Unconjugated] [NBP2-62015] - Western blot of nonreduced(-) and reduced(+) rabbit IgG, using NBP2-62015. This antibody reacts to nonreduced rabbit IgG (~150 kDa).](http://images.novusbio.com/fullsize/IgG-Fc-Antibody-RMG02-Unconjugated-Western-Blot-NBP2-62015-img0004.jpg "Western Blot: IgG Fc Antibody (RMG02) [Unconjugated] [NBP2-62015] - Western blot of nonreduced(-) and reduced(+) rabbit IgG, using NBP2-62015. This antibody reacts to nonreduced rabbit IgG (~150 kDa).")
![ELISA: IgG Fc Antibody (RMG02) - Azide and BSA Free [NBP2-62015] - A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of RMG02 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62015_goat-monoclonal-igg-fc-antibody-rmg02-elisa-1892025233951.jpg)
![ELISA: IgG Fc Antibody (RMG02) - Azide and BSA Free [NBP2-62015] - ELISA of IgGs from different species shows RMG02 reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG02 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62015_goat-monoclonal-igg-fc-antibody-rmg02-elisa-189202523512120.jpg)
![SDS-Page: Goat F(ab')2 anti-Rabbit IgG Fc Secondary Antibody [NB120-6016] - Lane 1: Reduced Goat F(ab')2 anti-Rabbit IgG Fc Secondary Antibody. Lane 2: Opal PreStained Molecular Weight Ladder . Lane 3: Non-reduced Goat F(ab')2 anti-Rabbit IgG Fc Secondary Antibody. Load: 1.0ug. 4-20% SDS Gel, Coomassie Blue Stained.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Rabbit-IgG-Fc-Secondary-Antibody-SDS-Page-NB120-6016-img0001.jpg "SDS-Page: Goat F(ab')2 anti-Rabbit IgG Fc Secondary Antibody [NB120-6016] - Lane 1: Reduced Goat F(ab')2 anti-Rabbit IgG Fc Secondary Antibody. Lane 2: Opal PreStained Molecular Weight Ladder . Lane 3: Non-reduced Goat F(ab')2 anti-Rabbit IgG Fc Secondary Antibody. Load: 1.0ug. 4-20% SDS Gel, Coomassie Blue Stained.")
![Western Blot: Donkey anti-Rabbit IgG Fc Secondary Antibody [NBP1-72766] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (DONKEY) Antibody 1:5,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-Fc-Secondary-Antibody-[Unconjugated]-Western-Blot-NBP1-72766-img0004.jpg "Western Blot: Donkey anti-Rabbit IgG Fc Secondary Antibody [NBP1-72766] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (DONKEY) Antibody 1:5,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Dot Blot: Rabbit anti-Human IgG Fc Secondary Antibody [NBP2-60675] - Dot Blot results of [FITC]. Dots are Human IgG: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocked for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Human-IgG-Fc-Secondary-Antibody-Dot-Blot-NBP2-60675-img0003.jpg "Dot Blot: Rabbit anti-Human IgG Fc Secondary Antibody [NBP2-60675] - Dot Blot results of [FITC]. Dots are Human IgG: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocked for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the FITC version of this antibody.")
![ELISA: Rabbit anti-Human IgG Fc Secondary Antibody [NBP2-60675] - ELISA results tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 ug of Human IgG F(c). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer and TMB substrate. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Human-IgG-Fc-Secondary-Antibody-ELISA-NBP2-60675-img0002.jpg)
![ELISA: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Tested against purified Goat IgG F(c) (purple line), Goat IgG (green line). Each well was coated in duplicate with 1.0 ug. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay was performed using blocking buffer, donkey anti-Rabbit HRP and TMB ELISA Peroxidase substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-ELISA-NBP2-60668-img0001.jpg "ELISA: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Tested against purified Goat IgG F(c) (purple line), Goat IgG (green line). Each well was coated in duplicate with 1.0 ug. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay was performed using blocking buffer, donkey anti-Rabbit HRP and TMB ELISA Peroxidase substrate.")
![Fluorophore-linked immunosorbent assay: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60668-img0003.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60668-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60668-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Goat IgG Fc Secondary Antibody [NBP2-60668] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Goat-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60668-img0006.jpg)
![Dot Blot: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773] - Antigen: Dog IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Dog IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image using the Alkaline Phosphatase form of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Canine-IgG-Fc-Secondary-Antibody-Dot-Blot-NBP1-72773-img0001.jpg "Dot Blot: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773] - Antigen: Dog IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Dog IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image using the Alkaline Phosphatase form of this antibody.")
![ELISA: Rabbit anti-Canine IgG Fc Secondary Antibody [NBP1-72773] - ELISA results of purified Rabbit anti-Canine IgG Fc Secondary antibody tested against purified Canine IgG F(c). Each well was coated in duplicate with 1.0 ug of Canine IgG F(c) (red line), Canine IgG (green line) .The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Gt Anti-Rabbit HRP and substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Canine-IgG-Fc-Secondary-Antibody-ELISA-NBP1-72773-img0004.jpg)