Rabbit IgG, Fc Native Protein

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SDS-Page: Rabbit IgG, Fc Native Protein [NBP1-97061] - SDS-PAGE of Rabbit IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC, PAGE
Concentration
1.1 mg/ml

Order Details

Rabbit IgG, Fc Native Protein Summary

Description
A full-length protein corresponding to Rabbit IgG, Fc.

Source: Serum

Preparation
Method
Rabbit IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rabbit IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(c). No reaction was observed against anti-Rabbit IgG F(ab')2 or anti-Papain.
Protein/Peptide Type
Native Protein
Purity
Ion exchange chromatography

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.1 mg/ml
Purity
Ion exchange chromatography

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer possesses the epitope recognition site. Rabbit IgG F(c) fragment is ideal for investigators in Immunology, Cancer, and Microbiology research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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