Rabbit anti-Human IgG Fc Secondary Antibody [HRP]

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ELISA: Rabbit anti-Human IgG Fc Secondary Antibody [HRP] [NBP1-73529] - ELISA results of purified Rabbit anti-Human IgG F(c) Antibody Peroxidase Conjugated tested against purified Human IgG F(c). Each well was coated in ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP
Concentration
LYOPH

Order Details

Rabbit anti-Human IgG Fc Secondary Antibody [HRP] Summary

Immunogen
Human IgG F(c) fragment
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab')2
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
Application Notes
Suitable for immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency
Readout System

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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