Rabbit anti-Canine IgG Fc Secondary Antibody [Alkaline Phosphatase]

Images

 
Dot Blot: Rabbit anti-Canine IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72647] - Canine IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.

Product Details

Summary
Reactivity CaSpecies Glossary
Applications WB, DB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alkaline Phosphatase
Concentration
1 mg/ml

Order Details

Rabbit anti-Canine IgG Fc Secondary Antibody [Alkaline Phosphatase] Summary

Immunogen
Dog IgG F(c) fragment
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Dog IgG, Dog IgG F(c) and Dog Serum.  No reaction was observed against Dog IgG F(ab')2.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2500
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:200 - 1:1000
Application Notes
This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 - 1:44,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reacts with Canine.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Notes

Label Molecular Weight: 140,000

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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