Rabbit anti-Human IgG Fc Secondary Antibody [Biotin]


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Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
LYOPH mg/ml

Order Details

Rabbit anti-Human IgG Fc Secondary Antibody [Biotin] Summary

Human IgG F(c) fragment
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against Human IgG F(ab')2.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Biotin (A=N/A, E=N/A)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
0.01% Sodium Azide
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


Application Notes
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:15,000 - 1:60,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reacts with Human.


Label Molecular Weight: 455, Restore with 1.0 mL of deionized water (or equivalent).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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