Rabbit anti-Human IgG Fc Secondary Antibody

Images

 
Dot Blot: Rabbit anti-Human IgG Fc Secondary Antibody [NBP2-60675] - Dot Blot results of [FITC]. Dots are Human IgG: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocked for 1hr at RT. Imaged with ...read more
ELISA: Rabbit anti-Human IgG Fc Secondary Antibody [NBP2-60675] - ELISA results tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 ug of Human IgG F(c). The starting dilution of antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, DB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Rabbit anti-Human IgG Fc Secondary Antibody Summary

Immunogen
Rabbit anti-Human IgG Fc fragment Secondary Antibody was produced by repeated immunization with Human IgG F(c) fragment in rabbit.
Specificity
No reaction was observed against Human IgG F(ab).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA 1:375000
  • Immunohistochemistry 1:1000-1:6000
  • Western Blot 1:3000-1:30000
Application Notes
This secondary antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Publications
Read Publication using NBP2-60675.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Multi-step

Notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Fc Antibody (NBP2-60675)(1)

We have publications tested in 1 application: ELISA.


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Video Protocols

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