Rabbit anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase]

Dot Blot: Rabbit anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72623] - Antigen: Human IgG Fc Load: Lane 1 - 100 ng Lane 2 - 33.33 ng Lane 3 - 11.11 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng Incubated for 1 HR at RT; blocked for 1 HR at RT. Visualized using an alkaline phosphatase substrate.

• Reactivity: Hu
• Applications: WB, DB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alkaline Phosphatase

Rabbit anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase] Summary

• Description: Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum
• Immunogen: Rabbit anti-Human IgG Fc fragment Secondary Antibody was produced by repeated immunization with Human IgG F(c) fragment in rabbit.
• Specificity: No reaction was observed against Human IgG F(ab).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Western Blot 1:500 - 1:2500
• Application Notes: This product has been tested by dot blot and ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.05% Sodium Azide
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.