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![Western Blot: Phosphoserine Antibody [NB100-1953] - Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserineprotein using Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953). Primary Antibody: Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1uM okadaic acid).](http://images.novusbio.com/fullsize/Phosphoserine-Antibody-Western-Blot-NB100-1953-img0012.jpg "Western Blot: Phosphoserine Antibody [NB100-1953] - Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserineprotein using Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953). Primary Antibody: Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1uM okadaic acid).")
![Immunocytochemistry/Immunofluorescence: Phosphoserine Antibody [NB100-1953] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:50 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.](http://images.novusbio.com/fullsize/Phosphoserine-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1953-img0008.jpg)
![Western Blot: Phosphoserine Antibody [NB100-1953] - ~30 ug SY5Y or N2A whole cell lysate separated on 8% PAGE, blotted and probed with phosphoserine antibody (1:125 in 3% BSA in TBST). Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Phosphoserine-Antibody-Western-Blot-NB100-1953-img0011.jpg)
![Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405] - Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine. The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate, ExtrAvidin, Peroxidase Conjugate and AEC substrate.Lane A: Antibody dilution 1:1000Lane B: Negative control (only secondary antibody)](http://images.novusbio.com/fullsize/Phosphoserine-Antibody-PSR-45-Western-Blot-NB600-405-img0003.jpg "Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405] - Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine. The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate, ExtrAvidin, Peroxidase Conjugate and AEC substrate.Lane A: Antibody dilution 1:1000Lane B: Negative control (only secondary antibody)")
![Western Blot: Phosphoserine Antibody [HRP] [NB100-1981] - Phosphoserine Antibody [NB100-1981] - Western blot analysis of human MMRU melanoma cells probed with Phosphoserine polyclonal antibody. Top (from the left): MMRU cells treated with 0 (A), 50 (B), 200 (C), 400 (D) l long wavelength UV, or 0.1uM of okadaic acid (E) Bottom: Same samples, except probed with b-actin antibody](http://images.novusbio.com/fullsize/Phosphoserine-Antibody-[HRP]-Western-Blot-NB100-1981-img0001.jpg "Western Blot: Phosphoserine Antibody [HRP] [NB100-1981] - Phosphoserine Antibody [NB100-1981] - Western blot analysis of human MMRU melanoma cells probed with Phosphoserine polyclonal antibody. Top (from the left): MMRU cells treated with 0 (A), 50 (B), 200 (C), 400 (D) l long wavelength UV, or 0.1uM of okadaic acid (E) Bottom: Same samples, except probed with b-actin antibody")
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Analysis in human fallopian tube and skeletal muscle tissues using NBP1-87203 antibody. Corresponding Phosphoserine phosphatase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Analysis in human fallopian tube and skeletal muscle tissues using NBP1-87203 antibody. Corresponding Phosphoserine phosphatase RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Phosphoserine phosphatase Antibody [NBP1-87203] - Analysis in human cell lines A-431 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Western-Blot-NBP1-87203-img0009.jpg)
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0012.jpg)
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0013.jpg)
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0014.jpg)
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Staining of human skeletal muscle shows no postivity in myocytes as expected.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0015.jpg)
![Western Blot: Phosphoserine phosphatase Antibody [NBP1-56848] - Analysis of PSPH in human PSPH overexpressed glioma (Lane1) and non-transfected (Lane2) cells using anti-PSPH (Phosphoserine phosphatase) antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Western-Blot-NBP1-56848-img0003.jpg "Western Blot: Phosphoserine phosphatase Antibody [NBP1-56848] - Analysis of PSPH in human PSPH overexpressed glioma (Lane1) and non-transfected (Lane2) cells using anti-PSPH (Phosphoserine phosphatase) antibody. Image from verified customer review.")
![Western Blot: Phosphoserine phosphatase Antibody [NBP1-56848] - Human Heart lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Western-Blot-NBP1-56848-img0002.jpg)
![Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - PSPH was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg HepG2 Whole Cell Lysate. 0.5 uL anti-PSPH rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PSPH rabbit monoclonal antibody,at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-001-Western-Blot-NBP2-90222-img0005.jpg "Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - PSPH was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg HepG2 Whole Cell Lysate. 0.5 uL anti-PSPH rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PSPH rabbit monoclonal antibody,at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions.")
![Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Immunofluorescence staining of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PSPH monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-001-Immunocytochemistry-Immunofluorescence-NBP2-90222-img0003.jpg)
![Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Anti-PSPH rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-001-Western-Blot-NBP2-90222-img0004.jpg)
![Immunoprecipitation: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - PSPH was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg HepG2 Whole Cell Lysate. 0.5 uL anti-PSPH rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PSPH rabbit monoclonal antibody,at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 25 kDa. Observed band size: 25 kDa.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-001-Immunoprecipitation-NBP2-90222-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody [NBP2-47625] - Staining human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47625-img0002.jpg "Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody [NBP2-47625] - Staining human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP2-47625] - Staining of human small intestine shows strong cytoplasmic positivity with a granular pattern in glandular cells.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-47625-img0001.jpg)
![Western Blot: Phosphoserine phosphatase Antibody (3G12) [NBP1-04297] - Cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04297 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: Jurkat cell lysate. Lane 2: K562 cell lysate. Lane 3: HepG2 cell lysate. Lane 4: A549 cell lysate. Lane 5: MCF7 cell lysate](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-3G12-Western-Blot-NBP1-04297-img0001.jpg "Western Blot: Phosphoserine phosphatase Antibody (3G12) [NBP1-04297] - Cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04297 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: Jurkat cell lysate. Lane 2: K562 cell lysate. Lane 3: HepG2 cell lysate. Lane 4: A549 cell lysate. Lane 5: MCF7 cell lysate")
![Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody (3G12) [NBP1-04297] - A431 cells stained with NBP1-04297 (1:100). Alexa Fluor 488 - conjugated secondary antibody was used (green). DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-3G12-Immunocytochemistry-Immunofluorescence-NBP1-04297-img0002.jpg)
![Western Blot: Phosphoserine phosphatase Antibody [NBP1-28916] - (0.05ug/ml) Analysis of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Western-Blot-NBP1-28916-img0002.jpg "Western Blot: Phosphoserine phosphatase Antibody [NBP1-28916] - (0.05ug/ml) Analysis of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Phosphoserine phosphatase Antibody (3A5) [H00005723-M01] - Analysis of PSPH expression in transfected 293T cell line by PSPH monoclonal antibody (M01), clone 3A5.Lane 1: PSPH transfected lysate(25 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-3A5-Western-Blot-H00005723-M01-img0005.jpg "Western Blot: Phosphoserine phosphatase Antibody (3A5) [H00005723-M01] - Analysis of PSPH expression in transfected 293T cell line by PSPH monoclonal antibody (M01), clone 3A5.Lane 1: PSPH transfected lysate(25 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Phosphoserine phosphatase Antibody (3A5) [H00005723-M01] - PSPH monoclonal antibody (M01), clone 3A5. Analysis of PSPH expression in human placenta.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-3A5-Western-Blot-H00005723-M01-img0004.jpg)
![Sandwich ELISA: Phosphoserine phosphatase Antibody (3A5) [H00005723-M01] - Detection limit for recombinant GST tagged PSPH is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-3A5-Sandwich-ELISA-H00005723-M01-img0003.jpg)
![Western Blot: Phosphoserine phosphatase Antibody (2G9) [H00005723-M02] - Analysis of PSPH expression in transfected 293T cell line by PSPH monoclonal antibody (M02), clone 2G9.Lane 1: PSPH transfected lysate(25 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-2G9-Western-Blot-H00005723-M02-img0006.jpg "Western Blot: Phosphoserine phosphatase Antibody (2G9) [H00005723-M02] - Analysis of PSPH expression in transfected 293T cell line by PSPH monoclonal antibody (M02), clone 2G9.Lane 1: PSPH transfected lysate(25 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Phosphoserine phosphatase Antibody (2G9) [H00005723-M02] - PSPH monoclonal antibody (M02), clone 2G9 Analysis of PSPH expression in K-562.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-2G9-Western-Blot-H00005723-M02-img0005.jpg)
![Sandwich ELISA: Phosphoserine phosphatase Antibody (2G9) [H00005723-M02] - Detection limit for recombinant GST tagged PSPH is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-2G9-Sandwich-ELISA-H00005723-M02-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody [NBP2-52738] - Staining of human cell line A-431 shows positivity in cytoplasm.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52738-img0001.jpg "Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody [NBP2-52738] - Staining of human cell line A-431 shows positivity in cytoplasm.")
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP2-52738] - Staining of human small intestine shows strong cytoplasmic positivity with a granular pattern in glandular cells.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-52738-img0002.jpg)