419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND₅₀ is typically 0.04-0.3 µg/mL." class="big_lightbox"> 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND₅₀ is typically 0.04-0.3 µg/mL." alt="Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND₅₀ is typically 0.04-0.3 µg/mL." class="big_thumb" />

499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB4991) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB4991) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB1510). The ND₅₀ is typically 0.02-0.1 µg/mL. This antibody also neutralizes Human IL-12 induced activity in human PBLs. The ND50 is approximately 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Human IL-12." class="big_lightbox"> 402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB1510). The ND₅₀ is typically 0.02-0.1 µg/mL. This antibody also neutralizes Human IL-12 induced activity in human PBLs. The ND50 is approximately 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Human IL-12." alt="In the presence of Recombinant Mouse IL-2 (10 ng/mL, Catalog # 402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB1510). The ND₅₀ is typically 0.02-0.1 µg/mL. This antibody also neutralizes Human IL-12 induced activity in human PBLs. The ND50 is approximately 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Human IL-12." class="big_thumb" />