| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50416-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP1-97358 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26049 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21862-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26050 | |||
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-363-img0005.jpg "Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.")
![Western Blot: HA Tag Antibody [NB600-363] - 200, 100, or 50 ng of E. coli whole cell lysate expressing amulti-tag fusion protein.Antibody: Affinity purified rabbitanti-HA antibody used for WB at 0.04 ug/ml(1:25,000). Detection: Chemiluminescence with anexposure time of 3 seconds.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-363-img0019.jpg)
![Western Blot: HA Tag Antibody [NB600-363] - Wnt/beta-catenin signaling regulates HySp5 expression. Immunoprecipitation (IP) of HA-tagged HySp5-420 expressed in HEK293T cells together or not with hudeltabeta-Catenin (upper) or huTCF1 (lower). IP was performed with an anti-HA antibody and Co-IP products were detected with the anti-beta-catenin or anti-TCF1 antibodies. Same results were obtained in two independent experiments. Each data point represents one biological independent experiment. Statistical p values: *p <= 0.05, **p <= 0.01, ***p <= 0.001, ****p <= 0.0001 (unpaired t test). Error bars indicate SD. PP primer pair, RLA relative luciferase activity Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-08242-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-363-img0018.jpg)
![Western Blot: HA Tag Antibody [NB600-363] - ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases. PLoS One (2011)](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-363-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - HA-tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1 ug/mL rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals). Cells were stained using the appropriate secondary antibody, donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-363-img0013.jpg)
![Western Blot: HA Tag Antibody [NB600-363] - Gene knockout in U2OS.693 cells.(A) Schematic of ZFN-mediated knockout. A ZFN pair (ZFNR & ZFNL) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway & hence leads to disruption of the coding sequence. (B) Dose-dependent gene disruption in U2OS.693 cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average & standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. (C) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors & pEGFP, cell lysates were probed with antibodies against the HA tag & EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, & 1200 ng. NT, non-transfected cells. (D) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average & standard deviation). WT, E502-WT; OH, E502-OH. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22194948), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-363_rabbit-polyclonal-ha-tag-antibody-310202416171456.jpg)
![Immunohistochemistry: HA Tag Antibody [NB600-362] - SUMO1 conjugates are not localized to amyloid plaques. (a) Sagittal brain sections of 24-week-old KI/AD and WT/AD animals were stained using anti-HA antibodies (red), 6E10 antibodies (green) that label amyloid beta 1-42 among other amyloid beta variants (epitope lies within amino acids 3-8 of amyloid beta) and anti-MAP2 antibodies (blue). Sections of the hippocampal subiculum are shown. Images are representatives of three independent experiments. Scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12760) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunohistochemistry-NB600-362-img0011.jpg "Immunohistochemistry: HA Tag Antibody [NB600-362] - SUMO1 conjugates are not localized to amyloid plaques. (a) Sagittal brain sections of 24-week-old KI/AD and WT/AD animals were stained using anti-HA antibodies (red), 6E10 antibodies (green) that label amyloid beta 1-42 among other amyloid beta variants (epitope lies within amino acids 3-8 of amyloid beta) and anti-MAP2 antibodies (blue). Sections of the hippocampal subiculum are shown. Images are representatives of three independent experiments. Scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12760) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: HA Tag Antibody [NB600-362] - Lysates of HEK293T human embryonic kidney cell line transfected with N-terminal HA-tagged MICA, N-terminal HA-tagged LGI-2, mock transfected, C-terminal HA-tagged LRRN5, and C-terminal HA-tagged Trance. PVDF membrane was probed with 1:500 dilution of 1:2500 dilution of goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1:5000 dilution of rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018).](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-362-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-362] - HA-tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1 ug/mL rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals). Cells were stained using the appropriate secondary antibody, donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-362-img0005.jpg)
![Western Blot: HA Tag Antibody - BSA Free [NB600-362] - 200, 67, 22, 7, or 2 ng of E. coli whole cell lysateexpressing a multi-tag fusion protein. Antibodies: Affinitypurified, goat anti-HA antibody used for WBat 0.04 ug/ml (1:25,000). Detection: Chemiluminescencewith an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/HA-Tag-Antibody---BSA-Free-Western-Blot-NB600-362-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-362] - HA tagged RFX6 overexpression in HEK293 stained with HA Tag antibody at a dilution of 1:250. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-NB600-362-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714] - HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA stained by HA tag antibody [4810] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-4810-Immunocytochemistry-Immunofluorescence-NBP2-43714-img0006.jpg "Immunocytochemistry/Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714] - HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA stained by HA tag antibody [4810] diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Human HEK293T cells overexpressed with Ly6K-HA. Dution 1:1000. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-4810-Western-Blot-NBP2-43714-img0005.jpg)
![Flow Cytometry: HA Tag Antibody (4810) [NBP2-43714] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted from a verified customer review.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-4810-Flow-Cytometry-NBP2-43714-img0004.jpg)
![Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of 293T whole cell lysate/extract A: 20 ug whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C: Immunoprecipitation of HA tagged protein by 2.5 ug of HA tagg antibody 10% SDS-PAGE. The immunoprecipitated HA tagged protein was detected by HA tag antibody [4810] diluted at 1:3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-4810-Western-Blot-NBP2-43714-img0002.jpg)
![Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of A: 30 ug HeLa whole cell lysate/extract B: 30 ug whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [4810] dilution: 1:5000.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-4810-Western-Blot-NBP2-43714-img0003.jpg)
![Western Blot: HA Tag Antibody [NBP1-70006] - Detection of 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-Western-Blot-NBP1-70006-img0004.jpg "Western Blot: HA Tag Antibody [NBP1-70006] - Detection of 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.")
![Immunohistochemistry-Paraffin: HA Tag Antibody [NBP1-70006] - Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 um were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-Immunohistochemistry-Paraffin-NBP1-70006-img0001.jpg)
![Western Blot: HA Tag Antibody (RM305) [NBP2-61477] - 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61477 at 0.1 ug/ml](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RM305-Western-Blot-NBP2-61477-img0003.jpg "Western Blot: HA Tag Antibody (RM305) [NBP2-61477] - 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61477 at 0.1 ug/ml")
![Immunohistochemistry-Paraffin: HA Tag Antibody (RM305) [NBP2-61477] - Staining of LNCaP cells overexpressing HA-tag Bag1 protein, using NBP2-61477 at 0.01 ug/ml](http://images.novusbio.com/fullsize/HA-Tag-Antibody-RM305-Immunohistochemistry-Paraffin-NBP2-61477-img0001.jpg)
![Western Blot: HA Tag Antibody (SR2318) [NBP3-21862] - Western blot analysis of HA Tag fusion protein, using NBP3-21862.](http://images.novusbio.com/fullsize/nbp3-21862_rabbit-ha-tag-mab-sr2318-57202314565413.jpg "Western Blot: HA Tag Antibody (SR2318) [NBP3-21862] - Western blot analysis of HA Tag fusion protein, using NBP3-21862.")
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2C11-Western-Blot-H00008878-M01-img0012.jpg "Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).")
![<b>Genetic Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of p62 in p62 WT and KO mouse hepatocytes and HepG2 cell lysate using anti-p62/SQSTM1 antibody clone 2C11. Sample lane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells. This image is from a verified customer product review.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2C11-Western-Blot-H00008878-M01-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - ICC-IF analysis of SQSTM1 protein in HeLa cell using SQSTM1/p62 antibody (clone 2C11) at 10 ug/ml concentration.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2C11-Immunocytochemistry-Immunofluorescence-H00008878-M01-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62 in SH-SY5Y cells using anti-p62/SQSTM1 antibody. Red - p62 puncta; Blue - nuclear DAPI; Green - Cytoskeleton. Image from verified customer review.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2C11-Immunocytochemistry-Immunofluorescence-H00008878-M01-img0010.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Disruption of the VANGL2-p62/SQSTM1 interaction in breast cancer cells. Soft agar colony formation of T47D cells overexpressing GFP, GFP-VANGL2 and GFP-p62/SQSTM1 (right). Protein expression was revealed with anti-p62/SQSTM1, anti-VANGL2 and anti-GFP antibodies by western blot analysis (right). In anti-GFP blot, the arrowhead indicates position of co-migrating GFP-VANGL2 and GFP-p62/SQSTM1 and the asterisk pinpoints GFP alone. Error bars represent mean+/-s.d. (n=3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms10318), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2C11-Western-Blot-H00008878-M01-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62/SQSTM1 antibody on HeLa cells. Image from verified customer review.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2C11-Immunocytochemistry-Immunofluorescence-H00008878-M01-img0016.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Confirmation of loss of hepatic ATG7 protein and a phenotype of hepatic autophagy inactivation. Fourth ug of liver protein were loaded on 4-20% gradient Tris-Glycine gels and subsequently immunoblotted with antibodies against ATG7, p62 and LC3. Blots were visualized with G:BOX Chemi XRQ (SynGene). Representative immunoblots are shown for WT and KO mice. Full-length immunoblots are presented in Supplementary Figure S1. ATG7: autophagy-related protein 7; p62: sequestosome 1; LC3: microtubule-associated protein 1 light chain 3; WT: Mx1-Cre-Atg7F/F mouse; KO: Mx1-Cre+Atg7F/F mouse. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14405-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2C11-Western-Blot-H00008878-M01-img0015.jpg)
![ELISA: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Detection limit for recombinant GST tagged SQSTM1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2C11-ELISA-H00008878-M01-img0013.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Activation of multiple signaling pathways by dandelion root extractA. Western blots of proteins involved in programmed cell death & cell survival & inflammation B. Densitometry quantification of western blot analysis from three independent experiments. *P<0.05, **P< 0.001. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11485), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-31020241534382.jpg)
![Immunohistochemistry: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Elevated levels of additional proteins & GM3 ganglioside in the MEC of MPS IIIB brain.Staining performed with antibodies to the indicated substances was observed in the MEC region of 3 month-old MPS IIIB mice (for total ubiquitin & polyubiquitin) & 6 months for all others. Staining was not seen in the MEC region of age-matched control mice (Naglu +/−) nor in the LEC region of MPS IIIB mice (the latter not shown). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0027461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-310202415392568.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Confirmation of loss of hepatic ATG7 protein & a phenotype of hepatic autophagy inactivation. Fourty µg of liver protein were loaded on 4–20% gradient Tris-Glycine gels & subsequently immunoblotted with antibodies against ATG7, p62 & LC3. Blots were visualised with G:BOX Chemi XRQ (SynGene). Representative immunoblots are shown for WT & KO mice. Full-length immunoblots are presented in Supplementary Figure S1. ATG7: autophagy-related protein 7; p62: sequestosome 1; LC3: microtubule-associated protein 1 light chain 3; WT: Mx1-Cre−Atg7F/F mouse; KO: Mx1-Cre+Atg7F/F mouse. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29074879), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-310202415345313.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - The content of mitochondrial p62, OPTN, & VCP in spinal cord of SOD1‐G93A mice is decreased by Parkin knockoutImmunostaining of anterior horn of lumbar spinal cord for ChAT (in green) & p62 (in red) at 130 days. Scale bar, 10 μm.Quantification of the percentage of ChAT‐positive motor neurons (MN) containing p62‐positive inclusions at 130 days. PKO/G93A MN have less SOD1 aggregates than G93A, while Non Tg & PKO MN have none. Data were collected from n = 4 (two males & two females) mice for Non Tg & PKO & n = 6 (three males & three females) for G93A & PKO/G93A. Number of images recorded for each genotype: n = 9 for Non Tg & PKO, n = 16 for G93A, & n = 12 for PKO/G93A. Results are expressed as mean ± SEM; *P = 0.032, by unpaired one‐tailed Student's t‐test.Representative Western blots of OPTN, p62, & VCP in spinal cord mitochondrial fractions at 130 days. Protein levels are normalized by Complex V. The quantifications in panels (D–F) show that mitochondria of PKO/G93A mice have less mitophagy adaptor proteins than G93A relative to Complex V.Quantification of OPTN relative to Complex V at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); *P = 0.039 by paired Student's t‐test.Quantification of p62, relative to Complex V at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); **P = 0.0029 by paired Student's t‐test.VCP was quantified using Complex V as loading reference at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); *P = 0.044 by paired Student's t‐test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30126943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-310202415482234.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - miR-29a downregulates TFEB & ATG9A to inhibit autophagyA. Schematic representation of the miR-29 family members & 3′-UTR binding sites of miR-29 targets as well as mutated binding sites used in Luciferase Assays: Transcription Factor EB (TFEB) & Autophagy-related protein 9A (ATG9A). All three miR-29 family members (miR-29a, miR-29b, & miR-29c) have identical seed sequences. Conserved miR-29 binding sites in the 3′-UTR of mRNA transcripts encoding ATG9A & TFEB are depicted in bold. B. 10ug of total protein cell lysates from Panc-1 transfected with CTRL or miR-29a mimics were subjected to western blot analysis for TFEB, ATG9A, & GAPDH. Relative quantification of band intensities normalized to GAPDH are shown below respective blots. C. Relative firefly luciferase activity from TFEB & ATG9A 3′ UTR wild type (WT) & mutant (mut) reporter constructs following co-transfection into Panc-1 cells with control or miR-29a mimics. All readouts were normalized to renilla luciferase activity for each well. Average relative luminesce normalized to respective controls is presented (n=6) ± S.E.M. D. 5ug of total protein cell lysates from Panc-1 cells were transfected with CTRL, miR-29a mimics, siCTRL, siTFEB, or siATG9A. 24 hours post-transfection, total protein was harvested & subjected to western blot analysis for p62 & LC3B, & GAPDH was used as loading control. Quantification of band intensities normalized to GAPDH & relative to control are shown below respective blots. All experiments were repeated 3 times & representative data is presented. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11928), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-310202415483626.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Effects of autophagy inhibitors in combination with IFN-gamma on expression of PD-L1 in gastric cancer cell lines. a The effect of chloroquine (CQ) or 3-MA on expression of PD-L1 with or without INF-gamma for 24 h was determined by flow cytometry assays. In AGS & NCI-n87 cells, MFI as the indication of PD-L1 expression level can be further increased by the treatment of INF-gamma . b Levels of LC3B-I/II, p62/SQSTM1 & PD-L1 were determined by Western blots in AGS & NCI-n87 cells treated by CQ, 3-MA, bafilomycin A1 (Baf) or rapamycin (Rap) for 24 h. c Positive staining of PD-L1 (red) & LC3 positive puncta (green) was determined by immunofluorescence in AGS & NCI-n87 cells treated by autophagy inhibitors & activator as in (b). d Rapamycin decreased the levels of PD-L1 protein in AGS & NCI-n87 cells as shown by flow cytometry. Results were averaged & blots were representative of 4 independent experiments, *p < 0.05, **p < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30925913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-31020241548511.jpg)
![Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - The content of mitochondrial p62, OPTN, & VCP in spinal cord of SOD1‐G93A mice is decreased by Parkin knockoutImmunostaining of anterior horn of lumbar spinal cord for ChAT (in green) & p62 (in red) at 130 days. Scale bar, 10 μm.Quantification of the percentage of ChAT‐positive motor neurons (MN) containing p62‐positive inclusions at 130 days. PKO/G93A MN have less SOD1 aggregates than G93A, while Non Tg & PKO MN have none. Data were collected from n = 4 (two males & two females) mice for Non Tg & PKO & n = 6 (three males & three females) for G93A & PKO/G93A. Number of images recorded for each genotype: n = 9 for Non Tg & PKO, n = 16 for G93A, & n = 12 for PKO/G93A. Results are expressed as mean ± SEM; *P = 0.032, by unpaired one‐tailed Student's t‐test.Representative Western blots of OPTN, p62, & VCP in spinal cord mitochondrial fractions at 130 days. Protein levels are normalized by Complex V. The quantifications in panels (D–F) show that mitochondria of PKO/G93A mice have less mitophagy adaptor proteins than G93A relative to Complex V.Quantification of OPTN relative to Complex V at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); *P = 0.039 by paired Student's t‐test.Quantification of p62, relative to Complex V at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); **P = 0.0029 by paired Student's t‐test.VCP was quantified using Complex V as loading reference at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); *P = 0.044 by paired Student's t‐test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30126943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-310202415482212.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - G2019S LRRK2 hippocampal neurons do not have elevated alpha -synuclein pathology 14 days post-transduction. a Primary hippocampal neurons from NTG or G2019S pups were transduced with 2.5 μg/mL alpha -synuclein PFFs & allowed to age a further 14 days prior to sequential detergent fractionation. TX-100-insoluble alpha -synuclein & p62 are similar in both neuron types. b Quantification of soluble proteins shows ~ 25-fold elevation in the expression of LRRK2 & a commensurate ~ 50-fold elevation in pS395 LRRK2, indicative of the elevated LRRK2 kinase activity associated with the G2019S mutation. Soluble alpha -synuclein levels were equivalent between the cultures. c No significant differences were found between the genotypes in insoluble proteins by an unpaired t-test with Welch’s correction. (N = 3 biological replicates for each protein). Means + s.e.m.; **P < 0.01; *P < 0.05 by an unpaired t-test with Welch’s correction for unequal variances. All values are normalized to NTG neurons treated with alpha -synuclein PFFs & DMSO Image collected & cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0550-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-31020241548515.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - LRRK2 inhibition does not reduce insoluble alpha -synuclein in wildtype hippocampal neurons. a Primary hippocampal neurons from CD1 pups were treated with 30 nM LRRK2 inhibitors PF-475, PF-360 or DMSO as a vehicle control, then transduced with 2.5 μg/mL alpha -synuclein PFFs & allowed to age a further 14 days prior to sequential detergent fractionation. TX-100-insoluble alpha -synuclein & p62 are similar in both LRRK2 inhibitor treated & untreated neurons. b Quantification of soluble proteins show some reduction of LRRK2 protein levels PF-475 treatment & ~ 75% inhibition of LRRK2 activity (as assayed by pS935 LRRK2) by both PF-475 & PF-360. c Insoluble pS129 alpha -synuclein was slightly, but significantly elevated by PF-360 treatment, while alpha -synuclein & p62 were unchanged by one-way ANOVA. (N = 5 biological replicates for each protein). Means + s.e.m.; *P < 0.05, ****p < 0.0001 by one-way ANOVA with Dunnett’s multiple comparison test. All values are normalized first to GAPDH, as a loading control, then to DMSO-treated neurons Image collected & cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0550-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-310202415485130.jpg)
![Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Colocalization of VANGL2 & p62/SQSTM1 in late endosomes.(a) Immunofluorescence staining of SKBR7 cells showed colocalization of endogenous VANGL2 (green) & p62/SQSTM1 (red) in discrete cytoplasmic puncta. Scale bar, 10 μm. The mean Pearson correlation for VANGL2 & p62/SQSTM1 is 0.62, calculated using the Image J software for ∼15 cells per field of view & from 10 images. (b) Partial colocalization of VANGL2 & p62/SQSTM1 in late endosomes of SKBR7 cells stained w/ the LAMP1 marker. Scale bar, 20 μm. (c) SKBR7 ells cultured in a nutrient-deprived medium & treated w/ 100 nM bafilomycin A1 (6 h) before fixation. Double labelling against VANGL2 (arrowheads) & p62/SQSTM1 (arrows), as described in Methods, showed accumulations of both markers in vesicular structures probably resembling endosomes/amphisomes. Scale bar, 200 nm. (d) IMCD3 cells treated w/ PBS or EGTA (5 mM) for 30 min. Immunofluorescence & confocal analysis performed using the indicated antibodies. Scale bar, 10 μm. Inserts show colocalized VANGL2 & p62/SQSTM1. (e) The VANGL2–p62/SQSTM1 complex recovered in confluent SKBR7 or IMCD3 cells w/ 2G4 mAb (IP VANGL2) but not a control antibody (IP IgG2a ctrl) as seen using western blot analysis w/ the indicated antibodies. The complex more abundant in cancer cells (SKBR7) than in polarized cells (IMCD3). Note that human (SKBR7 cells) & murine (IMCD3 cells) p62/SQSTM1 run at different molecular weights. (f) Lysates of confluent IMCD3 cells treated for 30 min w/ PBS or EGTA subjected to 2G4 mAb immunoprecipitation (IP VANGL2). Immunoprecipitated proteins probed by western blot analysis w/ the indicated antibodies. Increased amounts of VANGL2–p62/SQSTM1 complexes recovered after EGTA treatment. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms10318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-310202415482274.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Colocalization of VANGL2 & p62/SQSTM1 in late endosomes.(a) Immunofluorescence staining of SKBR7 cells showed colocalization of endogenous VANGL2 (green) & p62/SQSTM1 (red) in discrete cytoplasmic puncta. Scale bar, 10 μm. The mean Pearson correlation for VANGL2 & p62/SQSTM1 is 0.62, calculated using the Image J software for ∼15 cells per field of view & from 10 images. (b) Partial colocalization of VANGL2 & p62/SQSTM1 in late endosomes of SKBR7 cells stained w/ the LAMP1 marker. Scale bar, 20 μm. (c) SKBR7 ells cultured in a nutrient-deprived medium & treated w/ 100 nM bafilomycin A1 (6 h) before fixation. Double labelling against VANGL2 (arrowheads) & p62/SQSTM1 (arrows), as described in Methods, showed accumulations of both markers in vesicular structures probably resembling endosomes/amphisomes. Scale bar, 200 nm. (d) IMCD3 cells treated w/ PBS or EGTA (5 mM) for 30 min. Immunofluorescence & confocal analysis performed using the indicated antibodies. Scale bar, 10 μm. Inserts show colocalized VANGL2 & p62/SQSTM1. (e) The VANGL2–p62/SQSTM1 complex recovered in confluent SKBR7 or IMCD3 cells w/ 2G4 mAb (IP VANGL2) but not a control antibody (IP IgG2a ctrl) as seen using western blot analysis w/ the indicated antibodies. The complex more abundant in cancer cells (SKBR7) than in polarized cells (IMCD3). Note that human (SKBR7 cells) & murine (IMCD3 cells) p62/SQSTM1 run at different molecular weights. (f) Lysates of confluent IMCD3 cells treated for 30 min w/ PBS or EGTA subjected to 2G4 mAb immunoprecipitation (IP VANGL2). Immunoprecipitated proteins probed by western blot analysis w/ the indicated antibodies. Increased amounts of VANGL2–p62/SQSTM1 complexes recovered after EGTA treatment. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms10318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-310202415483640.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Parkin protein levels are unaffected in liver while a small decrease is measured in G93A cerebellumRepresentative Western blot for Parkin & p62 in cerebellum (Cb) homogenates at 130 days.Quantification indicates that Parkin is decreased in G93A mice relative to Non Tg at 130 days. Protein levels were normalized by beta ‐actin. Results are expressed as mean ± SEM & percent of Non Tg; n = 8 (four males & four females) mice per group. *P = 0.039 by paired Wilcoxon's test.Quantification of p62 in the homogenates indicates that p62 is increased in PKO & PKO/G93A mice, independent of SOD1‐G93A expression, at 130 days of age. beta ‐actin was used for normalization. Results are expressed as mean ± SEM & percent of Non Tg; n = 8 (four males & four females) mice per group. No statistically significant differences were found between Non Tg & G93A by paired Friedman's test with Dunn's correction (P = 0.99). ***P = 0.0001 by paired Student's t‐test (G93A vs. PKO/G93A).Representative Western blot of Parkin & p62 in liver homogenates at 130 days.Parkin protein levels were quantified at 130 days. beta ‐actin was used for normalization. Results are expressed as mean ± SEM & percent of Non Tg; n = 8 (four males & four females) mice per group. No statistically significant differences were found between Non Tg & G93A (P = 0.546 by paired Wilcoxon's test).Quantification of p62 protein levels in liver at 130 days. Results are expressed as mean ± SEM & percent of Non Tg; n = 8 (four males & four females) mice per group. No statistically significant differences were found between Non Tg & G93A (P = 0.546 by paired Wilcoxon's test). No statistically significant differences were found among the other groups.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30126943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-310202415482248.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Parkin knockout mitigates the accumulation of mitophagy adaptors in G93A mitochondria at disease end stageA, BRepresentative Western blots of p62 (A), OPTN, & VCP (B) in spinal cord mitochondria from end‐stage mice.Cp62 quantification, using Complex V as normalizer, shows a strong accumulation of p62 in SOD1‐G93A mitochondria at end stage. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females) mice per group. No statistically significant differences were found between G93A & PKO/G93A (P = 0.078 by paired Wilcoxon's test); ***P = 0.0007 (Non Tg vs. G93A) & *P = 0.037 (PKO & PKO/G93A) both by paired Friedman's test with Dunn's correction.DQuantification of OPTN accumulation in end‐stage mitochondria, with Complex V as protein loading control. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females) mice per group. **P = 0.0078 (for G93A & PKO/G93A) by paired Wilcoxon's test; ***P = 0.0003 (Non Tg vs. G93A) by paired Friedman's test with Dunn's correction. No other statistically significant differences were found.EQuantification of VCP in mitochondria at disease end stage. Complex V was used as normalizer. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females) mice per group. *P = 0.039 (for G93A & PKO/G93A) by paired Wilcoxon's test; ***P = 0.0007 (Non Tg vs. G93A) & *P = 0.037 (PKO & PKO/G93A) both by paired Friedman's test with Dunn's correction.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30126943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008878-m01_mouse-monoclonal-p62-sqstm1-antibody-2c11-31020241548226.jpg)
![Western Blot: HA-1 Antibody (4H7) [H00023526-M06] - Analysis of HMHA1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/HA-1-Antibody-4H7-Western-Blot-H00023526-M06-img0002.jpg "Western Blot: HA-1 Antibody (4H7) [H00023526-M06] - Analysis of HMHA1 expression in Raw 264.7.")
![Sandwich ELISA: HA-1 Antibody (4H7) [H00023526-M06] - Detection limit for recombinant GST tagged HMHA1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HA-1-Antibody-4H7-Sandwich-ELISA-H00023526-M06-img0001.jpg)
![Western Blot: HA-1 Overexpression Lysate (Adult Normal) [NBP2-10359] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HA-1.](http://images.novusbio.com/fullsize/HA-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10359-img0001.jpg "Western Blot: HA-1 Overexpression Lysate (Adult Normal) [NBP2-10359] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HA-1.")