HA Tag Antibody (543851) [Unconjugated] - Peptide

Western blot shows Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP, 5 ng/lane). PVDF membrane was probed with 1 µg/mL of Mouse Anti-HA Tag Peptide Antibody Monoclonal Antibody (Catalog # MAB060) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for N-terminal HA-tagged Recombinant Human APRIL/ TNFSF13 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: All-Multi
• Applications: WB
• Clone: 543851
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HA Tag Antibody (543851) [Unconjugated] - Peptide Summary

• Immunogen: HA Tag Peptide
  Accession # ABB51961
• Specificity: Specifically detects N-terminal HA-tagged proteins with a free N-terminus.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HA Tag Antibody (543851) [Unconjugated] - Peptide

• HA Epitope Tag
• HA Tag
• HA
• Hemagglutinin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA Tag Antibody (MAB060)(11)

Publications using MAB060

• Dobaño-López, C;Valero, JG;Araujo-Ayala, F;Nadeu, F;Gava, F;Faria, C;Norlund, M;Morin, R;Bernes-Lasserre, P;Arenas, F;Grau, M;López, C;López-Oreja, I;Serrat, N;Martínez-Farran, A;Hernández, L;Playa-Albinyana, H;Giménez, R;Beà, S;Campo, E;Lagarde, JM;López-Guillermo, A;Magnano, L;Colomer, D;Bezombes, C;Pérez-Galán, P; Patient-derived follicular lymphoma spheroids recapitulate lymph node signaling and immune profile uncovering galectin-9 as a novel immunotherapeutic target Blood cancer journal 2024-05-02 [PMID: 38697976] (Human)
• Thomas J. Wubben, Sraboni Chaudhury, Brennan T. Watch, Jeanne A. Stuckey, Eric Weh, Roshini Fernando et al. Development of Novel Small-Molecule Activators of Pyruvate Kinase Muscle Isozyme 2, PKM2, to Reduce Photoreceptor Apoptosis Pharmaceuticals (Basel) 2023-05-06 [PMID: 37242488] (Neutralization, Mouse)
• Acharya BR, Fang JS, Jeffery ED et al. Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Life science alliance 2023-08-01 [PMID: 37197981]
• Thomas J. Wubben, Mercy Pawar, Eric Weh, Andrew Smith, Peter Sajjakulnukit, Li Zhang et al. Small molecule activation of metabolic enzyme pyruvate kinase muscle isozyme 2, PKM2, circumvents photoreceptor apoptosis Scientific Reports 2020-02-19 [PMID: 32076076]
• Jae-Wook Lee, Dong Hee Yang, Sojin Park, Hae-Kyoung Han, Jong-Wan Park, Bo Yeon Kim et al. Trichostatin A resistance is facilitated by HIF-1 alpha acetylation in HeLa human cervical cancer cells under normoxic conditions Oncotarget 2018-01-05 [PMID: 29416751]
• J Dai, MA Luftig Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation J. Immunol., 2018-01-22;0(0):. 2018-01-22 [PMID: 29358277] (Functional Assay, Human)
• L Grossman, C Chang, J Dai, PA Nikitin, DD Jima, SS Dave, MA Luftig Epstein-Barr Virus Induces Adhesion Receptor CD226 (DNAM-1) Expression during Primary B-Cell Transformation into Lymphoblastoid Cell Lines mSphere, 2017-11-29;2(6):. 11/29/2017 [PMID: 29202043]
• Mercy Pawar, Boris Busov, Aaruran Chandrasekhar, Jingyu Yao, David N Zacks, Cagri G Besirli FAS apoptotic inhibitory molecule 2 is a stress-induced intrinsic neuroprotective factor in the retina Cell Death & Differentiation 2017-10-01 [PMID: 28708137]
• AM Price, J Dai, Q Bazot, L Patel, PA Nikitin, R Djavadian, PS Winter, CA Salinas, AP Barry, KC Wood, EC Johannsen, A Letai, MJ Allday, MA Luftig Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection Elife, 2017-04-20;6(0):. 2017-04-20 [PMID: 28425914] (Human)
• Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer B, Kuppers R Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions. Proc Natl Acad Sci U S A, 2015-01-26;112(6):E546-55. 2015-01-26 [PMID: 25624468] (Cross-linking, Human)
• Nikitin , Pavel A, Price , Alexande, McFadden , Karyn, Yan , Christop, Luftig , Micah A Mitogen-induced B-cell proliferation activates Chk2-dependent G1/S cell cycle arrest. PLoS ONE, 2014-01-30;9(1):e87299. 2014-01-30 [PMID: 24498068] (Cross-linking, Human)

FAQs for HA Tag Antibody (MAB060). (Showing 1 - 1 of 1 FAQs).

1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
   • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

Bioinformatics

• Uniprot:
  • ABB51961()