HA Tag Antibody

Images

 
Western Blot: HA Epitope Tag Antibody [NBP1-70006] - Detection of1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate ...read more
Immunohistochemistry-Paraffin: HA Epitope Tag Antibody [NBP1-70006] - Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 um were prepared from representative paraffin ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ChIP, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

HA Tag Antibody Summary

Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy ter
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
20mM Sodium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • ELISA 1:20000 - 1:150000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used - identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused - the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

Reactivity Notes

HA

Alternate Names for HA Tag Antibody

  • HA Epitope Tag
  • HA Tag
  • HA
  • Hemagglutinin

Background

Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for HA Tag Antibody (NBP1-70006). (Showing 1 - 1 of 1 FAQ).

  1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
    • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

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