HA Tag Antibody (1049F) [HRP]

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 1:1000-1:3000 dilution of Rabbit Anti-HA Tag Peptide HRP-conjugated Monoclonal Antibody (Catalog # HAM0601). Specific bands were detected for HA-tagged proteins at approximately 70 kDa and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: All-Multi
• Applications: WB
• Clone: 1049F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: HRP

HA Tag Antibody (1049F) [HRP] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: HA Tag Peptide
  Accession # ABB51961
• Specificity: Detects N-terminal and C-terminal HA-tagged proteins in Western blot.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:3000 dilution

Packaging, Storage & Formulations

• Storage: Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied as a solution in PBS containing a preservative.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HA Tag Antibody (1049F) [HRP]

• HA Epitope Tag
• HA Tag
• HA
• Hemagglutinin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA Tag Antibody (HAM0601)(3)

Publications using HAM0601

• I Sahu, SM Mali, P Sulkshane, C Xu, A Rozenberg, R Morag, MP Sahoo, SK Singh, Z Ding, Y Wang, S Day, Y Cong, O Kleifeld, A Brik, MH Glickman The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag Nature Communications, 2021-10-26;12(1):6173. 2021-10-26 [PMID: 34702852] (Western Blot, Human)
• MM Papasergi-, MJ Robertson, AB Seven, O Panova, JM Mathiesen, G Skiniotis Structures of metabotropic GABAB receptor Nature, 2020-06-24;0(0):. 2020-06-24 [PMID: 32580208] (ELISA (detection), Human)
• Ioanna Panagi, Elliott Jennings, Jingkun Zeng, Regina A. Günster, Cullum D. Stones, Hazel Mak et al. Salmonella Effector SteE Converts the Mammalian Serine/Threonine Kinase GSK3 into a Tyrosine Kinase to Direct Macrophage Polarization Cell Host & Microbe 2020-01-08 [PMID: 31862381]

FAQs for HA Tag Antibody (HAM0601). (Showing 1 - 1 of 1 FAQs).

1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
   • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

Bioinformatics

• Uniprot:
  • ABB51961()