HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_lightbox">
HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." alt="Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (
HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_thumb" />
HAF017). A specific band was detected for Brachyury at approximately 50 kDa (as indicated). GAPDH (
AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">
HAF017). A specific band was detected for Brachyury at approximately 50 kDa (as indicated). GAPDH (
AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />
AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (
BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (
MAG999) and DNA was purified using chelating resin solution. The
VEGFpromoter was detected by standard PCR." class="big_lightbox">
AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (
BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (
MAG999) and DNA was purified using chelating resin solution. The
VEGFpromoter was detected by standard PCR." class="big_thumb" />
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
F0107). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012). View our protocol for
Staining Intracellular Molecules." class="big_lightbox">
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
F0107). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012). View our protocol for
Staining Intracellular Molecules." class="big_thumb" />
BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells." alt="As an alternative, please consider our next generation Recombinant Human IL-2 (BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells." class="big_thumb" />
HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />
![N/A CXCL8/IL-8 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL8_DY208_ELISA_2307.jpg "N/A CXCL8/IL-8 [Biotin]")
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_CXCL8_IL8_D8050_ELISA_557.jpg "N/A CXCL8/IL-8 [HRP]")
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_CXCL8_IL8_D8050_ELISA_558.jpg)
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/elisa/CXCL8_D8000C_ELISA_378.jpg)
![Toll-like receptor 4 (TLR4) silencing decreases endogenous levels of pro-inflammatory cytokines. a Effect of TLR2/4 silencing in breast cancer cells transfected with negative control (nc) siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2) was analyzed using quantitative real-time PCR; ***P <0.001 (analysis of variance (ANOVA)). b IL-6 (left) and IL-8 (right) ELISA on supernatants from MDA-MB-231 breast cancer cells transfected with nc siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2); n = 4. Error bars standard error of the mean (SEM); *P <0.05, **P <0.01, ***P <0.001 (ANOVA). c Boyden chamber migration assays. Migration of primary human myeloid cells towards supernatants from different cell lines indicated. Human primary peripheral blood mononuclear cells were isolated as previously described [48] and allowed to migrate through a Costar Transwell® Permeable Support 8.0-μm 24-well plate (Corning) to the supernatants of breast cancer supernatants cultured under serum-free conditions. Percentage of migrated CD11b+ cells was analyzed using a flow cytometer and CD11b-APC antibodies (BD Sciences); n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (ANOVA). d Matrigel invasion assays. Invasion of lipopolysaccharide (LPS)-stimulated/un-stimulated MDA-MB-231 cells into matrigel invasion chambers (BD Sciences) as indicated: 25 × 103 MDA-MB-231 cells were stimulated or not with LPS and allowed to invade from 72 h. Amount of invaded cells was analyzed using crystal violet staining and manual counting in four separate experiments; n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (Student’s t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/d8000c_human-il-8-cxcl8-quantikine-elisa-kit-22820241795613.jpg)
HAF017). A specific band was detected for Brachyury at approximately 50 kDa (as indicated). GAPDH (
Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
NL001) and counter-stained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for 
NL001) and counter-stained with DAPI (blue). View our protocol for 
AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # 
![Flow Cytometry: WC1(+) gamma delta T cell Antibody (BAQ89A) [NBP2-61056] - Obtained with bovine leukocytes](http://images.novusbio.com/fullsize/WC1-+-gamma-delta-T-cell-Antibody-BAQ89A-Flow-Cytometry-NBP2-61056-img0001.jpg "Flow Cytometry: WC1(+) gamma delta T cell Antibody (BAQ89A) [NBP2-61056] - Obtained with bovine leukocytes")
![Flow Cytometry: WC1(+) gamma delta T cell Antibody (GB45A) [NBP2-61045] - Obtained with caprine leukocytes](http://images.novusbio.com/fullsize/WC1-+-gamma-delta-T-cell-Antibody-GB45A-Flow-Cytometry-NBP2-61045-img0001.jpg "Flow Cytometry: WC1(+) gamma delta T cell Antibody (GB45A) [NBP2-61045] - Obtained with caprine leukocytes")
![Flow Cytometry: WC1(+) gamma delta T cell Antibody (GB54A) [NBP2-61047] - Obtained with bovine leukocytes](http://images.novusbio.com/fullsize/WC1-+-gamma-delta-T-cell-Antibody-GB54A-Flow-Cytometry-NBP2-61047-img0001.jpg "Flow Cytometry: WC1(+) gamma delta T cell Antibody (GB54A) [NBP2-61047] - Obtained with bovine leukocytes")
![Flow Cytometry: WC1(+) gamma delta T cell Antibody (BAG25A) [NBP2-61080] - Obtained with bovine leukocytes](http://images.novusbio.com/fullsize/WC1-+-gamma-delta-T-cell-Antibody-BAG25A-Flow-Cytometry-NBP2-61080-img0001.jpg "Flow Cytometry: WC1(+) gamma delta T cell Antibody (BAG25A) [NBP2-61080] - Obtained with bovine leukocytes")
