T Cell Exhaustion Marker (Flow) Antibody Pack

Flow Cytometry: T Cell Exhaustion Marker (Flow) - Human peripheral blood lymphocytes were stained with Mouse Anti-Human TIGIT Monoclonal Antibody (Catalog # MAB7898) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog# F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog# FAB2408P). Quadrant markers were set based on control antibody staining (Catalog# MAB0041).

Flow Cytometry: T Cell Exhaustion Marker (Flow) - Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog#: FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (Catalog# MAB6944or (B) Normal Rabbit IgG Control (Catalog# AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# F0110) at 10 uL/10^6 cells.

Flow Cytometry: T Cell Exhaustion Marker (Flow) - Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 ng/mL PHA for 2 days or (B) untreated, were stained with Rabbit Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10863) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# F0111) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog# FAB100P). Quadrant markers were set based on control antibody staining (Catalog# MAB1050).

Flow Cytometry: T Cell Exhaustion Marker (Flow) - NS0 mouse myeloma cell line transfected with human CTLA-4 and eGFP was stained with (A) Rabbit Anti-Human CTLA-4 Monoclonal Antibody (Catalog # MAB3253) or (B) Normal Rabbit IgG Control Antibody (Catalog# MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog# F0111).

Flow Cytometry: T Cell Exhaustion Marker (Flow) - A cell surface stain was performed on U-937 cells with VISTA/B7-H5/PD-1H (730823) antibody MAB71263PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready

T Cell Exhaustion Marker (Flow) Antibody Pack Summary

• Description: The Human T Cell Exhaustion Marker Sample Pack (Flow) contains antibodies to detect expression of inhibitory receptors associated with T cell exhaustion including PD-1, CTLA-4, TIGIT, TIM-3, LAG-3 and VISTA. Immune cells expressing these inhibitory receptors are found during chronic viral infection and within the tumor microenvironment of many solid tumors. Also included are three PE-Conjugated secondary antibodies for fluorescent detection by flow cytometry.All antibodies have been validated for use in flow cytometry applications.
  
  This pack contains 1 vial each of:
  MAB10863-SP (25 ug):
  MAB3253-SP (25 ug)
  MAB7898-SP (25 ug)
  MAB2365R-SP (25 ug)
  MAB71263-SP (25 ug)
  MAB23191-SP (25 ug)
  F0110 (25ug)
  (100 Tests)
  F0102B (100 Tests)
  F0105B (100 Tests)
• Immunogen: See individual datasheets
• Marker: T cell exhaustion
• Specificity: Please see each vial's data sheet for specificity information specific to each product.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
• Application Notes: See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Packaging, Storage & Formulations

• Storage: Storage is content dependent.
• Buffer: See individual datasheets

Alternate Names for T Cell Exhaustion Marker (Flow) Antibody Pack

• exhaustion markers
• immune cell exhaustion
• immune checkpoint markers
• inhibitory receptors
• T cell exhaustion

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.