Product Search for GLUT1: Novus Biologicals

NB110-39113

Glut1 Antibody - BSA Free

(7 Reviews)

(70 Publications)

0.1 ml

NB110-39113

Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Immunohistochemical analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.

Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NB110-39113] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective

Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza (PMID: 21620138).

Western Blot: Glut1 Antibody [NB110-39113] - Glut1 in human brain lysate (55 kDa). Antibody at 1:500. Western blot image submitted by a verified customer review.

<b>Biological Strategies Validation. </b>Flow Cytometry: Glut1 Antibody [NB110-39113] - Flow cytometry analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. FMO control (red) and isotype control (blue, NBP2-24983) were compared to CD4+ T cells (orange), and this PE conjugated Glut 1 antibody positively stained CD4+ lymphocytes isolated from Mouse. Flow cytometry image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: Glut1 Antibody - BSA Free [NB110-39113] - LAIR-1 inhibits Glut1-related glucose uptake in OS cells. a Heatmap showing the levels of differentially expressed mRNAs. b Top 20 KEGG pathway annotation categories for target gene functions of predicted mRNAs. c Selected significantly differentially expressed mRNA-related to EMT in RNA-seq data between two groups, ***P < 0.001. d qPCR validation of differentially expressed EMT-related genes in LV-NC & LV-LAIR-1-overexpressing OS cells, **P < 0.01. e Glut1 expression analyzed by western blotting. f Immunofluorescence staining of Glut1 in the LV-LAIR-1-overexpressing OS cells. Scale bar = 50 μm. Data were obtained from at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32563267), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Glut1 Antibody - BSA Free [NB110-39113] - LAIR-1 inhibits Glut1-related glucose uptake in OS cells. a Heatmap showing the levels of differentially expressed mRNAs. b Top 20 KEGG pathway annotation categories for target gene functions of predicted mRNAs. c Selected significantly differentially expressed mRNA-related to EMT in RNA-seq data between two groups, ***P < 0.001. d qPCR validation of differentially expressed EMT-related genes in LV-NC & LV-LAIR-1-overexpressing OS cells, **P < 0.01. e Glut1 expression analyzed by western blotting. f Immunofluorescence staining of Glut1 in the LV-LAIR-1-overexpressing OS cells. Scale bar = 50 μm. Data were obtained from at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32563267), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Simple Western: Glut1 Antibody - BSA Free [NB110-39113] - Protein changes in ONHAs corroborate bioenergetics data. (A) Glucose transporter-1 protein levels in Stretched ONH astrocytes are significantly higher than Control (*p = 0.0225, n = 7 Control, n = 8 Stretch). Retinal lysate from a 2 month-old mouse was used as a positive control for each protein analyzed, while negative control was the signal obtained when no primary antibody was included in the capillary. (B) Lactate dehydrogenase-A, the astrocyte-specific isoform of the enzyme that catalyzes the interconversion of pyruvate & lactate, has equivalent protein levels in Control & Stretch ONH astrocytes. (C) Glucose-6-phosphate dehydrogenase, the enzyme that shunts glucose into the pentose phosphate pathway, is no different in Control & Stretch ONH astrocytes. (D) Glutamine synthetase, the enzyme that synthesizes glutamine from glutamate, is no different in Control & Stretch ONH astrocytes. (E) The monomeric form of glutamate-aspartate transporter (GLAST) has significantly higher protein levels in the Stretch as compared to the Control ONH astrocytes (p = 0.020; n = 4 Control, n = 5 Stretch). (F) GLAST dimer protein levels are no different in Control & Stretch ONH astrocytes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35992925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Rb, Bv, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Rb, Bv, Pm

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-39113AF350
Alexa Fluor 405	NB110-39113AF405
Alexa Fluor 488	NB110-39113AF488
Alexa Fluor 532	NB110-39113AF532
Alexa Fluor 594	NB110-39113AF594
Alexa Fluor 647	NB110-39113AF647
Alexa Fluor 700	NB110-39113AF700
Alexa Fluor 750	NB110-39113AF750
Alexa Fluor Plus 405	NB110-39113AFP405
Alexa Fluor Plus 488	NB110-39113AFP488
Alexa Fluor Plus 555	NB110-39113AFP555
Alexa Fluor Plus 594	NB110-39113AFP594
Alexa Fluor Plus 647	NB110-39113AFP647
Alexa Fluor Plus 680	NB110-39113AFP680
Allophycocyanin	NB110-39113APC
Allophycocyanin/Cy7	NB110-39113APCCY7
Biotin	NB110-39113B
CoraFluor 1	NB110-39113CL1
DyLight 350	NB110-39113UV
DyLight 405	NB110-39113V
DyLight 488	NB110-39113G
DyLight 550	NB110-39113R
DyLight 594	NB110-39113DL594
DyLight 650	NB110-39113C
DyLight 680	NB110-39113FR
DyLight 755	NB110-39113IR
FITC	NB110-39113F
HRP	NB110-39113H
Janelia Fluor 525	NB110-39113JF525
Janelia Fluor 549	NB110-39113JF549
Janelia Fluor 646	NB110-39113JF646
Janelia Fluor 669	NB110-39113JF669
PE	NB110-39113PE
PE/Atto594	NB110-39113PEATT594
PE/Cy5.5	NB110-39113PECY55
PE/Cy7	NB110-39113PECY7
PerCP	NB110-39113PCP
mFluor Violet 450 SE	NB110-39113MFV450
mFluor Violet 500 SE	NB110-39113MFV500
mFluor Violet 610 SE	NB110-39113MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-39113SS

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NB300-666

Glut1 Antibody

(2 Reviews)

(30 Publications)

0.1 mg

NB300-666

Western Blot: Glut1 Antibody [NB300-666] - Rat brain cortical extracts (10 ug total protein per lane) on 10% SDS-PAGE gel. Antibody diluted 1:2000.

Reactivity:

Hu, Mu, Rt, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ze

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB1418

Glut1 Antibody (202915) [Unconjugated]

(33 Publications)

200 μg

MAB1418

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox">

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

<b>Biological Strategies Validation. </b>Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # <a class=

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

NL007) and counterstained with DAPI(blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI(blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

TCR stimulation results in Glut-1 expression and concomitant glucose uptake in CD4 and CD8 lymphocytes: Induction of H1RBD and H2RBD binding. CD4+ and CD8+ T lymphocytes were isolated by negative selection and stimulated via the TCR using alpha CD3/ alpha CD28 mAbs. (A) Non-activated and TCR-activated T cells were used for binding assays with mAb1418 followed by incubation with a FITC-conjugated alpha mouse IgG. Intracellular Glut-1 levels were monitored in permeabilized cells using the C-term Glut-1 polyclonal antibody followed by incubation with a FITC-conjugated sheep alpha rabbit IgG antibody. Filled histograms depict binding in the presence of the secondary FITC-conjugated antibody alone. Expression of the HTLV-1 Env receptor was detected by a 30 min incubation of the non-activated and TCR-activated cells with rabbit rFc-tagged H1RBD fusion protein at 37°C and binding was revealed by a 20 min incubation at 4°C with a FITC-conjugated sheep alpha rabbit IgG antibody. Binding to the H2RBD domain fused directly to EGFP (H2RBD-EGFP) was detected following a 30 min incubation at 37°C. (B) Glucose uptake was assayed by incubating non-activated and TCR-activated CD4 and CD8 T cells (1 × 106) with 2-deoxy-D [1-3H]glucose (2 μCi) for 45 min at 37°C. Uptake for each cell population is expressed as mean counts per minute (CPM) for triplicate samples, error bars indicate SD. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/17504522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Endogenous Glut-1 expression in diverse cell types is not reflected by mAb1418 reactivity but correlates with binding of the HTLV-1 and HTLV-2 Env RBDs. (A) 293T, Jurkat and primary human erythrocytes were stained with mAb1418 and control binding with the secondary FITC-conjugated antibody is shown in all histograms (filled). Intracellular Glut-1 levels in permeabilized 293T and Jurkat cells were monitored with mAb1418 as well as the C-term polyclonal Glut-1 antibody. Expression of the HTLV-1 and HTLV-2 receptor was monitored by incubation of cells for 30 min at 37°C with the rFc-tagged H1RBD and H2RBD fusion proteins as well as the H2RBD-EGFP fusion protein. Binding is shown in solid line histograms whereas control immunofluorescence is shown in filled histograms. (B) Total Glut-1 protein levels in cell extracts from 293T, Jurkat and human erythrocytes were monitored by immunoblotting with an anti-C-term Glut-1 antibody. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/17504522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Antibody and HRBD binding following transfection of the Glut-1 and Glut-3 glucose transporters. (A) 293T cells were transfected with Glut-1 or Glut-3 expression vectors and assayed for binding to the mAb1418 and C-term anti-Glut-1 polyclonal Ab. The former stainings were performed on whole cells as well as permeabilized cells to determine cell surface and total binding, respectively. All stainings using the anti-Glut-1 pAb were performed on permeabilized cells as the recognized epitope is intracellular. Staining was performed at 4°C. Specific binding and background fluorescence due to the secondary conjugated Ab are indicated in solid line and filled histograms, respectively. (B) Control and transfected 293T cells were incubated with rFc-tagged H1RBD and H2RBD fusion proteins for 30 min at 37°C followed by incubation with a FITC-conjugated alpha rabbit-Fc antibody at 4°C. Direct binding to H2RBD was demonstrated by incubation of cells with an EGFP-tagged envelope (H2RBD-EGFP). Binding is shown in solid line histograms whereas control immunofluorescence is shown in filled histograms. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/17504522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of ethanol on CD4+ T cell glucose metabolism. (A) Representative FACS plots of GLUT1 expression in DIFF and DIFF + EtOH CD4+ T cells. (B) Ethanol increased CD4+ T cell GLUT1 expression. (C) Representative FACS histograms of 2-NBDG uptake in DIFF and DIFF + EtOH CD4+ T cells. (D) Ethanol increased glucose uptake within differentiated CD4+ T cells. (E) All experimental groups were analyzed using the Glycolytic Stress test. Ethanol increased glycolysis (F) and glycolytic capacity (G) within differentiated groups. MFI = mean fluorescent intensity, 2-NBDG = 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose, ECAR = extracellular acidification rate, Naïve = undifferentiated and no ethanol treatment, EtOH = undifferentiated and ethanol-treated, DIFF = differentiated and no ethanol treatment, and DIFF + EtOH = differentiated and ethanol-treated. Data represents average values using CD4+ T cells from 6 donors expressed as mean ± SEM. Significant differences (p<0.05) were determined by repeated measures 2-way ANOVA. *p ≤ 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35634279), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1418U-100UG
Alexa Fluor 405	FAB1418V-100UG
Alexa Fluor 488	FAB1418G-100UG
Alexa Fluor 532	FAB1418X
Alexa Fluor 594	FAB1418T-100UG
Alexa Fluor 700	FAB1418N
Alexa Fluor 750	FAB1418S-100UG
Alexa Fluor Plus 405	FAB1418AFP405-100UG
Alexa Fluor Plus 488	FAB1418AFP488-100UG
Alexa Fluor Plus 555	FAB1418AFP555-100UG
Alexa Fluor Plus 594	FAB1418AFP594-100UG
Alexa Fluor Plus 647	FAB1418AFP647-100UG
Alexa Fluor Plus 680	FAB1418AFP680-100UG
Allophycocyanin	FAB1418A
Allophycocyanin/Cy7	FAB1418APCCY7
Biotin	FAB1418B
CoraFluor 1	FAB1418CL1
DyLight 350	FAB1418D
DyLight 405	FAB1418E
DyLight 488	FAB1418K
DyLight 550	FAB1418L
DyLight 594	FAB1418M
DyLight 650	FAB1418W
DyLight 680	FAB1418Y
DyLight 755	FAB1418Z
Fluorescein	FAB1418F
HRP	FAB1418H
Janelia Fluor 525	FAB1418JF525
Janelia Fluor 549	FAB1418I
Janelia Fluor 646	FAB1418J
Janelia Fluor 669	FAB1418JF669
PE/Atto594	FAB1418PEATT594
PE/Cy5.5	FAB1418PECY55
PE/Cy7	FAB1418PECY7
Phycoerythrin	FAB1418P
mFluor Violet 450 SE	FAB1418MFV450
mFluor Violet 500 SE	FAB1418MFV500
mFluor Violet 610 SE	FAB1418MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1418A
Carrier Free	FAB1418N
Carrier Free	FAB1418F
Carrier Free	FAB1418P
Carrier Free	MAB1418-SP
Carrier Free	FAB1418V-100UG
Carrier Free	FAB1418U-100UG
Carrier Free	FAB1418T-100UG
Carrier Free	FAB1418S-100UG
Carrier Free	FAB1418G-100UG

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NBP2-75785

Glut1 Antibody (GLUT1/2476)

(1 Publication)

100 μg

NBP2-75785

Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2476).

Flow Cytometry: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Flow Cytometric Analysis of K562 cells using Glut1 Antibody (GLUT1/2476) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Protein Array: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-75787AF350
Alexa Fluor 405	NBP2-75787AF405
Alexa Fluor 488	NBP2-75787AF488
Alexa Fluor 532	NBP2-75787AF532
Alexa Fluor 594	NBP2-75787AF594
Alexa Fluor 647	NBP2-75787AF647
Alexa Fluor 700	NBP2-75787AF700
Alexa Fluor 750	NBP2-75787AF750
Alexa Fluor Plus 405	NBP2-75787AFP405
Alexa Fluor Plus 488	NBP2-75787AFP488
Alexa Fluor Plus 555	NBP2-75787AFP555
Alexa Fluor Plus 594	NBP2-75787AFP594
Alexa Fluor Plus 647	NBP2-75787AFP647
Alexa Fluor Plus 680	NBP2-75787AFP680
Allophycocyanin	NBP2-75787APC
Biotin	NBP2-75787B
CoraFluor 1	NBP2-75787CL1
DyLight 350	NBP2-75787UV
DyLight 405	NBP2-75787V
DyLight 488	NBP2-75787G
DyLight 550	NBP2-75787R
DyLight 594	NBP2-75787DL594
DyLight 650	NBP2-75787C
DyLight 680	NBP2-75787FR
DyLight 755	NBP2-75787IR
FITC	NBP2-75787F
HRP	NBP2-75787H
Janelia Fluor 525	NBP2-75787JF525
Janelia Fluor 549	NBP2-75787JF549
Janelia Fluor 646	NBP2-75787JF646
Janelia Fluor 669	NBP2-75787JF669
PE	NBP2-75787PE
PerCP	NBP2-75787PCP
mFluor Violet 450 SE	NBP2-75787MFV450
mFluor Violet 500 SE	NBP2-75787MFV500
mFluor Violet 610 SE	NBP2-75787MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-75787

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NBP2-75784

Glut1 Antibody (GLUT1/2475)

100 μg

NBP2-75784

Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human tongue stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2475).

Immunocytochemistry/Immunofluorescence: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Immunofluorescence staining of K562 cells using Glut1Mouse Monoclonal Antibody (GLUT1/2475) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are stained with Reddot.

Flow Cytometry: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Flow Cytometric analysis of K562 cells using Glut1 antibody followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-75786AF350
Alexa Fluor 405	NBP2-75786AF405
Alexa Fluor 488	NBP2-75786AF488
Alexa Fluor 532	NBP2-75786AF532
Alexa Fluor 594	NBP2-75786AF594
Alexa Fluor 647	NBP2-75786AF647
Alexa Fluor 700	NBP2-75786AF700
Alexa Fluor 750	NBP2-75786AF750
Alexa Fluor Plus 405	NBP2-75786AFP405
Alexa Fluor Plus 488	NBP2-75786AFP488
Alexa Fluor Plus 555	NBP2-75786AFP555
Alexa Fluor Plus 594	NBP2-75786AFP594
Alexa Fluor Plus 647	NBP2-75786AFP647
Alexa Fluor Plus 680	NBP2-75786AFP680
Allophycocyanin	NBP2-75786APC
Biotin	NBP2-75786B
CoraFluor 1	NBP2-75786CL1
DyLight 350	NBP2-75786UV
DyLight 405	NBP2-75786V
DyLight 488	NBP2-75786G
DyLight 550	NBP2-75786R
DyLight 594	NBP2-75786DL594
DyLight 650	NBP2-75786C
DyLight 680	NBP2-75786FR
DyLight 755	NBP2-75786IR
FITC	NBP2-75786F
HRP	NBP2-75786H
Janelia Fluor 525	NBP2-75786JF525
Janelia Fluor 549	NBP2-75786JF549
Janelia Fluor 646	NBP2-75786JF646
Janelia Fluor 669	NBP2-75786JF669
PE	NBP2-75786PE
PerCP	NBP2-75786PCP
mFluor Violet 450 SE	NBP2-75786MFV450
mFluor Violet 500 SE	NBP2-75786MFV500
mFluor Violet 610 SE	NBP2-75786MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-75786

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NBP3-15433

Glut1 Antibody (10O1I5)

100 ul

NBP3-15433

IHC-P- Glut1 Antibody (10O1I5) [NBP3-15433] - Mouse liver tissue using GLUT1/SLC2A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

IHC-P- Glut1 Antibody (10O1I5) [NBP3-15433] -Human pancreas tissue using GLUT1/SLC2A1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

IHC-P- Glut1 Antibody (10O1I5) [NBP3-15433] -Human placenta tissue using GLUT1/SLC2A1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: Glut1 Antibody (10O1I5) [Glut1] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Glut1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: Glut1 Antibody (10O1I5) [Glut1] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using Glut1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Human prostate cancer tissue using GLUT1/SLC2A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB14181

Glut1 Antibody (202921) [Unconjugated]

(3 Publications)

100 μg

MAB14181

HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glut1 at approximately 69 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Extracellular matrix stiffness modulates the expression of monocarboxylate transporters in mammary stromal cells. RMF 621 cells (2.5 × 105 per plate) were incubated on plates displaying increasing stiffness and coated with collagen I, as described above, in media +10% FCS. Afterward, 72 cells were lysed and Western blots of MCT4 (A) and Glut1 (B) were performed as described in Section 4. Densitometric analyses of three different experiments were performed from three independent experiments, as shown in (C). The data are presented as the means ± standard error (SEM). (*) and (**) indicate statistically significant differences, with p ≤ 0.05 and p ≤ 0.01, respectively, using the Friedman test followed by Dunn’s posthoc test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34357326), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB14181U-100UG
Alexa Fluor 405	FAB14181V-100UG
Alexa Fluor 488	FAB14181G-100UG
Alexa Fluor 532	FAB14181X-100UG
Alexa Fluor 594	FAB14181T-100UG
Alexa Fluor 647	FAB14181R-100UG
Alexa Fluor 700	FAB14181N-100UG
Alexa Fluor 750	FAB14181S-100UG
Alexa Fluor Plus 405	FAB14181AFP405-100UG
Alexa Fluor Plus 488	FAB14181AFP488-100UG
Alexa Fluor Plus 555	FAB14181AFP555-100UG
Alexa Fluor Plus 594	FAB14181AFP594-100UG
Alexa Fluor Plus 647	FAB14181AFP647-100UG
Alexa Fluor Plus 680	FAB14181AFP680-100UG
Allophycocyanin	FAB14181A
Biotin	FAB14181B
CoraFluor 1	FAB14181CL1
DyLight 350	FAB14181D
DyLight 405	FAB14181E
DyLight 488	FAB14181K
DyLight 550	FAB14181L
DyLight 594	FAB14181M
DyLight 650	FAB14181W
DyLight 680	FAB14181Y
DyLight 755	FAB14181Z
FITC	FAB14181F
HRP	FAB14181H
Janelia Fluor 525	FAB14181JF525
Janelia Fluor 549	FAB14181I
Janelia Fluor 646	FAB14181J
Janelia Fluor 669	FAB14181JF669
PE	FAB14181P
PerCP	FAB14181C
mFluor Violet 450 SE	FAB14181MFV450
mFluor Violet 500 SE	FAB14181MFV500
mFluor Violet 610 SE	FAB14181MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB14181-SP

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NBP3-32385

Glut1 Antibody (SA0377)

100 ul

NBP3-32385

Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.<br/><br/>Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB11601

Glut1 Antibody (1084409) [Unconjugated]

100 μg

MAB11601

HAF018). A specific band was detected for Glut1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for Glut1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glut1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface of HepG2 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line and absent in human PBMC cells using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface of HepG2 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Glut1 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Glut1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11601U-100UG
Alexa Fluor 405	FAB11601V-100UG
Alexa Fluor 488	FAB11601G-100UG
Alexa Fluor 532	FAB11601X-100UG
Alexa Fluor 594	FAB11601T-100UG
Alexa Fluor 647	FAB11601R-100UG
Alexa Fluor 700	FAB11601N-100UG
Alexa Fluor 750	FAB11601S-100UG
Alexa Fluor Plus 405	FAB11601AFP405-100UG
Alexa Fluor Plus 488	FAB11601AFP488-100UG
Alexa Fluor Plus 555	FAB11601AFP555-100UG
Alexa Fluor Plus 594	FAB11601AFP594-100UG
Alexa Fluor Plus 647	FAB11601AFP647-100UG
Alexa Fluor Plus 680	FAB11601AFP680-100UG
Allophycocyanin	FAB11601A
Biotin	FAB11601B
CoraFluor 1	FAB11601CL1
DyLight 350	FAB11601D
DyLight 405	FAB11601E
DyLight 488	FAB11601K
DyLight 550	FAB11601L
DyLight 594	FAB11601M
DyLight 650	FAB11601W
DyLight 680	FAB11601Y
DyLight 755	FAB11601Z
FITC	FAB11601F
HRP	FAB11601H
Janelia Fluor 525	FAB11601JF525
Janelia Fluor 549	FAB11601I
Janelia Fluor 646	FAB11601J
Janelia Fluor 669	FAB11601JF669
PE	FAB11601P
PerCP	FAB11601C
mFluor Violet 450 SE	FAB11601MFV450
mFluor Violet 500 SE	FAB11601MFV500
mFluor Violet 610 SE	FAB11601MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11601-SP

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NBP2-48609

Glut1 Antibody - BSA Free

0.1 ml

NBP2-48609

Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human cerebral cortex shows strong membranous positivity in endothelial cells.

Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Staining of human skin shows moderate membranous positivity in epidermal cells.

Immunohistochemistry: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of mouse brain shows moderate positivity in endothelial cells in the cerebral cortex.

Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human placenta shows strong membranous positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human testis shows no membranous positivity in cells in seminiferous ducts as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-48609-25ul

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NBP2-54745

Glut1 Antibody - BSA Free

(1 Publication)

100 ul

NBP2-54745

Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-54745] - Immunohistochemistry analysis of human placenta shows high expression.

Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-54745] - Immunohistochemistry analysis of human prostate shows low expression as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-54745-25ul

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NBP3-07887

Glut1 Antibody (GLUT1/3132R)

100 μg

NBP3-07887

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-08502AF350
Alexa Fluor 405	NBP3-08502AF405
Alexa Fluor 488	NBP3-08502AF488
Alexa Fluor 532	NBP3-08502AF532
Alexa Fluor 594	NBP3-08502AF594
Alexa Fluor 647	NBP3-08502AF647
Alexa Fluor 700	NBP3-08502AF700
Alexa Fluor 750	NBP3-08502AF750
Alexa Fluor Plus 405	NBP3-08502AFP405
Alexa Fluor Plus 488	NBP3-08502AFP488
Alexa Fluor Plus 555	NBP3-08502AFP555
Alexa Fluor Plus 594	NBP3-08502AFP594
Alexa Fluor Plus 647	NBP3-08502AFP647
Alexa Fluor Plus 680	NBP3-08502AFP680
Allophycocyanin	NBP3-08502APC
Biotin	NBP3-08502B
CoraFluor 1	NBP3-08502CL1
DyLight 350	NBP3-08502UV
DyLight 405	NBP3-08502V
DyLight 488	NBP3-08502G
DyLight 550	NBP3-08502R
DyLight 594	NBP3-08502DL594
DyLight 650	NBP3-08502C
DyLight 680	NBP3-08502FR
DyLight 755	NBP3-08502IR
FITC	NBP3-08502F
HRP	NBP3-08502H
Janelia Fluor 525	NBP3-08502JF525
Janelia Fluor 549	NBP3-08502JF549
Janelia Fluor 646	NBP3-08502JF646
Janelia Fluor 669	NBP3-08502JF669
PE	NBP3-08502PE
PerCP	NBP3-08502PCP
mFluor Violet 450 SE	NBP3-08502MFV450
mFluor Violet 500 SE	NBP3-08502MFV500
mFluor Violet 610 SE	NBP3-08502MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-08502

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Glut1 Antibody - BSA Free

Glut1 Antibody

Glut1 Antibody (202915) [Unconjugated]

Glut1 Antibody (GLUT1/2476)

Glut1 Antibody (GLUT1/2475)

Glut1 Antibody (10O1I5)

Glut1 Antibody (202921) [Unconjugated]

Glut1 Antibody (SA0377)

Glut1 Antibody (1084409) [Unconjugated]

Glut1 Antibody - BSA Free

Glut1 Antibody - BSA Free

Glut1 Antibody (GLUT1/3132R)