Product Search for ADAMTS: Novus Biologicals

AF5867

ADAMTS1 Antibody

100 μg

AF5867

HAF016). A specific band was detected for ADAMTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of P19 mouse embryonal carcinoma cell line and OVCAR-3 human ovarian carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for ADAMTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of P19 mouse embryonal carcinoma cell line and OVCAR-3 human ovarian carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage primordium; dorsal ganglia cell bodies and processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

ADAMTS1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage primordium; dorsal ganglia cell bodies and processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Immunohistochemical staining.(A) ADAMTS-1, (B) SMC marker alpha -actin, (C) macrophage marker in adventitial layer of human AAA aortas and (D) ADAMTS-1 in human non-aneurysmal control aortas. Staining of positive cells are seen in brown. The images are taken with 40X magnification and scale bar represent 50 μm. Red arrow indicates the same area. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28570682), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western blot analysis.(A) Expression of ADAMTS-1 and GAPDH in human AAA aortas (sample 1–6) and human non-aneurysmal control aortas (sample 7–11) and (B) quantification of ADAMTS-1 in relative values normalized to GAPDH. MWM: molecular weight marker indicating size in kDa. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28570682), licensed under a CC-BY license. Not internally tested by R&D Systems.

The metalloproteinase activity of ADAMTS1 is pivotal in stimulating the secretion of cleaved versican, activating the epidermal growth factor receptor (EGFR), promoting invasion, and conferring anoikis resistance in renal cell carcinoma (RCC) cells. A A schematic representation depicting two variants of the recombinant ADAMTS1 protein: one containing the metalloproteinase domain (ZnMc) and the other lacking it (TSP), along with a mutant ADAMTS1 expression construct (E402Q). B–E Treatment of Caki-1 cells with or without the recombinant ZnMc or TSP protein (40 nM) for 24 or 48 h. Subsequently, secretion of cleaved versican, activation of EGFR signal cascades, invasive abilities, and anoikis were respectively assessed using dot blot (B), western blotting (C), Matrigel invasion (D), and CCK8 assays. F–I Cleaved versican secretion (F), EGFR activation (G), invasive abilities (H), and anoikis (I) were evaluated in Caki-1 and 786-O cells after transducing wild-type (WT) ADAMTS1, ADAMTS1/E402Q, or a control vector. In D, E, H, and I, values are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, compared with the control group; #p < 0.05, ###p < 0.001, compared with the WT ADAMTS1-overexpressing group Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39333870), licensed under a CC-BY license. Not internally tested by R&D Systems.

ADAMTS1 expression promotes anoikis resistance of renal cell carcinoma (RCC) via inhibiting Bid, Bim, and Bak. A A western blot analysis was conducted to assess ADAMTS1 expression in Caki-1 and 786-O cells following transduction with either ADAMTS1 short hairpin (sh)RNA (left) or an ADAMTS1-expressing vector (right). B Cell viability of suspended Caki-1 and 786-O cells was evaluated using a CCK8 assay at 24 and 48 h post-stable overexpression (right) or knockdown (left) of ADAMTS1. Data are presented as the mean ± SD of three independent experiments. * p < 0.05, compared with the control group. C Dot plot demonstrated the correlation between the single sample gene set enrichment analysis (ssGSEA) score of “negative regulation of anoikis” and ADAMTS1 expression in TCGA-KIRC patients. A Pearson correlation was performed to evaluate their association and significance. D Western blot analysis of intrinsic apoptosis-related proteins (Bad, Bcl-2, Bak, Bid, Bim, and PARP) in suspended Caki-1 cells manipulated with ADAMTS1. GAPDH served as a loading control. E and F Dissemination of RCC cells in zebrafish embryos. Caki-1 cells with ADAMTS1-knockdown were implanted into zebrafish embryos at 48 h post fertilization. Tumor cell dissemination was observed at 2 days post injection (dpi), with disseminated tumor foci indicated by white arrowheads on the trunk and end-tail (E). Integrated densities of Caki-1 metastatic tumor cells in the zebrafish trunk and end-tail at 2 dpi were quantified, with the mean value of the integrated density in the shCtl group set to onefold (F) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39333870), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5867U-100UG
Alexa Fluor 405	AF5867V-100UG
Alexa Fluor 488	AF5867G-100UG
Alexa Fluor 532	AF5867X-100UG
Alexa Fluor 594	AF5867T-100UG
Alexa Fluor 647	AF5867R-100UG
Alexa Fluor 700	AF5867N-100UG
Alexa Fluor 750	AF5867S-100UG
Alexa Fluor Plus 405	AF5867AFP405-100UG
Alexa Fluor Plus 488	AF5867AFP488-100UG
Alexa Fluor Plus 555	AF5867AFP555-100UG
Alexa Fluor Plus 594	AF5867AFP594-100UG
Alexa Fluor Plus 647	AF5867AFP647-100UG
Alexa Fluor Plus 680	AF5867AFP680-100UG
Allophycocyanin	AF5867A
Biotin	BAF5867
CoraFluor 1	AF5867CL1
DyLight 350	AF5867D
DyLight 405	AF5867E
DyLight 488	AF5867K
DyLight 550	AF5867L
DyLight 594	AF5867M
DyLight 650	AF5867W
DyLight 680	AF5867Y
DyLight 755	AF5867Z
FITC	AF5867F
HRP	AF5867H
Janelia Fluor 525	AF5867JF525
Janelia Fluor 549	AF5867I
Janelia Fluor 646	AF5867J
Janelia Fluor 669	AF5867JF669
PE	AF5867P
PerCP	AF5867C
mFluor Violet 450 SE	AF5867MFV450
mFluor Violet 500 SE	AF5867MFV500
mFluor Violet 610 SE	AF5867MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5867-SP

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DY2198-05

Human ADAMTS5 DuoSet ELISA, 5 Plate

(16 Publications)

1 Kit (for 5 Plates)

DY2198-05

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP1-82916

ADAMTS9 Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-82916

Staining of human placenta shows moderate cytoplasmic positivity in decidual cells.

Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.

Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.

Staining of human prostate shows no positivity in smooth muscle cells as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-82916-25ul

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NBP2-15286

ADAMTS5 Antibody - BSA Free

(6 Publications)

0.1 ml

NBP2-15286

Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Untreated (–) and treated (+) RMS 13 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

MiR-23b-3p was the key mediator of osteocyte-derived extracellular vesicles in regulating cartilage metabolism.A A schematic diagram illustrating the separation of extracellular vesicles (EVs) and their enrichment from human tissues and mouse plasma. Heatmaps of relative miRNA differences (B) and enrichment analysis (C). D The miR-23b-3p levels in LS-EVs and HS-EVs (n = 5). E The miR-23b-3p levels in subchondral bone (SCB) osteocytes from WT and Dicer-cKO mice with destabilization of the medial meniscus (DMM) surgery (n = 5). F The miR-23b-3p levels in cartilage and SCB osteocytes from human LS/HS tissues (n = 20). G The miR-23b-3p levels in cartilage and SCB osteocytes from mice with DMM surgery (n = 6). H The pre-miR-23b-3p levels in cartilage and SCB osteocytes from human LS/HS tissues (n = 20). I The pre-miR-23b-3p levels in cartilage and SCB osteocytes from mice with DMM surgery (n = 6). J Alcian blue staining. Scale bar in upper panel, 1 mm. Scale bar in lower panel, 2 mm. K Western blot analysis of COLII, ACAN, SOX9, MMP13 and ADAMTS5 protein expression in primary human and mouse chondrocytes transfected with nc or mimic (n = 3). L The schematic depicted the overexpression of miR-23b-3p in cartilage through intraarticular injection of rAAV5-miR-23b-3p-mcherry (rAAV5-miR-23b-3p) or negative control (rAAV5-nc) in 8-week-old male mice following DMM surgery (n = 8). M The Safranin O images of cartilage. Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. Data are presented as the mean +/- SD. Data was analyzed by unpaired two-tailed Student’s t-test (D, E, G, I and K) and paired two-tailed Student’s t-test (F and H). Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40399261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Regulatory effects of Ept on catabolic and anabolic dynamics in hOACs. (A) Immunofluorescent staining against Col II and aggrecan (ACAN) in Ept or vehicle treated hOACs. (B) PCR results of Col II, ACAN, MMP-13, and ADAMTS5 (n = 3). (C) Representative western blot detection of ADAMTS5, ACAN, MMP-13, Col II, and beta -actin (n = 3). (D) Normalized quantitative data from western blot assay in Ept or vehicle treated hOACs. (E and F) Elisa detection of ADAMTS5 and MMP-13 levels in culture medium (n = 3). Mean +/- SD, p* < 0.05, p** < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36568290), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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DADT130

Human ADAMTS13 Quantikine ELISA Kit

(11 Publications)

1 Kit

DADT130

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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DY4307-05

Human ADAMTS4 DuoSet ELISA, 5 Plate

(12 Publications)

1 Kit (for 5 Plates)

DY4307-05

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-16038

ADAMTS13 Antibody (7B2Z8)

100 ul

NBP3-16038

Immunocytochemistry/ Immunofluorescence: ADAMTS13 Antibody (7B2Z8) [ADAMTS13] - Immunofluorescence analysis of paraffin-embedded rat liver using ADAMTS13 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: ADAMTS13 Antibody (7B2Z8) [ADAMTS13] - Immunofluorescence analysis of paraffin-embedded human liver using ADAMTS13 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: ADAMTS13 Antibody (7B2Z8) [ADAMTS13] - Immunofluorescence analysis of paraffin-embedded mouse liver using ADAMTS13 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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2198-AD

Recombinant Human ADAMTS5 Protein, CF

(12 Publications)

20 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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6156-AD

Recombinant Human ADAMTS13 (Full Length) Protein, CF

(10 Publications)

20 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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4307-AD

Recombinant Human ADAMTS4 Protein, CF

(10 Publications)

20 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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2197-AD

Recombinant Human ADAMTS1 Protein, CF

(8 Publications)

20 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB5149

ADAMTS15 Antibody (561819)

(1 Publication)

100 μg

MAB5149

HAF007). A specific band was detected for mature ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for mature ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for mature ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

ADAMTS15 was detected in immersion fixed paraffin-embedded sections of human kidney using Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

MCF-7 human breast cancer cell line was stained with Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149, filled histogram) or isotype control antibody (Catalog # <a class=

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5149U-100UG
Alexa Fluor 405	FAB5149V-100UG
Alexa Fluor 488	FAB5149G-100UG
Alexa Fluor 532	FAB5149X
Alexa Fluor 594	FAB5149T-100UG
Alexa Fluor 647	FAB5149R-100UG
Alexa Fluor 700	FAB5149N-100UG
Alexa Fluor 750	FAB5149S-100UG
Alexa Fluor Plus 405	FAB5149AFP405-100UG
Alexa Fluor Plus 488	FAB5149AFP488-100UG
Alexa Fluor Plus 555	FAB5149AFP555-100UG
Alexa Fluor Plus 594	FAB5149AFP594-100UG
Alexa Fluor Plus 647	FAB5149AFP647-100UG
Alexa Fluor Plus 680	FAB5149AFP680-100UG
Allophycocyanin	FAB5149A
Allophycocyanin/Cy7	FAB5149APCCY7
Biotin	FAB5149B
CoraFluor 1	FAB5149CL1
DyLight 350	FAB5149D
DyLight 405	FAB5149E
DyLight 488	FAB5149K
DyLight 550	FAB5149L
DyLight 594	FAB5149M
DyLight 650	FAB5149W
DyLight 680	FAB5149Y
DyLight 755	FAB5149Z
FITC	FAB5149F
HRP	FAB5149H
Janelia Fluor 525	FAB5149JF525
Janelia Fluor 549	FAB5149I
Janelia Fluor 646	FAB5149J
Janelia Fluor 669	FAB5149JF669
PE	FAB5149P
PE/Atto594	FAB5149PEATT594
PE/Cy5.5	FAB5149PECY55
PE/Cy7	FAB5149PECY7
PerCP	FAB5149C
mFluor Violet 450 SE	FAB5149MFV450
mFluor Violet 500 SE	FAB5149MFV500
mFluor Violet 610 SE	FAB5149MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5149-SP
Carrier Free	FAB5149V-100UG
Carrier Free	FAB5149U-100UG
Carrier Free	FAB5149T-100UG
Carrier Free	FAB5149S-100UG
Carrier Free	FAB5149R-100UG
Carrier Free	FAB5149N-100UG
Carrier Free	FAB5149G-100UG

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

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Clone

Gene Symbol

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Protein Or Peptide

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Promotional Item

Species Glossary

ADAMTS1 Antibody

Human ADAMTS5 DuoSet ELISA, 5 Plate

ADAMTS9 Antibody - BSA Free

ADAMTS5 Antibody - BSA Free

Human ADAMTS13 Quantikine ELISA Kit

Human ADAMTS4 DuoSet ELISA, 5 Plate

ADAMTS13 Antibody (7B2Z8)

Recombinant Human ADAMTS5 Protein, CF

Recombinant Human ADAMTS13 (Full Length) Protein, CF

Recombinant Human ADAMTS4 Protein, CF

Recombinant Human ADAMTS1 Protein, CF

ADAMTS15 Antibody (561819)