ZnT-8/SLC30A8 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

ZnT-8/SLC30A8 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human ZnT-8
  Lys268-Pro359
  Accession # Q8IWU4
• Specificity: Detects human Znt-8 in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZnT-8/SLC30A8 Antibody [Alexa Fluor® 350]

• SLC30A8
• solute carrier family 30 (zinc transporter), member 8
• Solute carrier family 30 member 8
• zinc transporter 8
• zinc transporter ZnT-8
• ZnT8
• ZnT-8
• ZNT8ZnT-8

Background

ZnT-8 (Zinc Transporter 8; also SLC30A8) is a 35-40 kDa member of the SLC (solute carrier)-30A subfamily, CDF family of proteins. It is expressed by pancreatic beta ‑cells and alpha -cells, B cells, and adipocytes, and is known to play a role in Zn transport. In particular, ZnT-8 appears to transport zinc from the cytosol into secretory vesicles which, in the case of beta -cells, provides a necessary component for proper insulin processing and granule storage. Further, it appears to facilitate glucose‑mediated insulin release. Human ZnT-8 is a multipass transmembrane (TM) protein that is 369 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-79) followed by six TM segments (aa 80-266) and a 103 aa C-terminal cytoplasmic tail. There is a key His-rich motif in the second cytoplasmic loop (aa 197‑205). ZnT-8 forms homodimers, and possibly oligomers. There is one alternative start site at Met50. Over aa 268-359, human ZnT-8 shares 79% aa sequence identity with mouse ZnT-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.